固定化脂质体色谱研究二至丸及其组成药物的经肠吸收成分

中国临床药理学与治疗学 ›› 2006, Vol. 11 ›› Issue (7): 776-779.

固定化脂质体色谱研究二至丸及其组成药物的经肠吸收成分

陈丙銮, 盛亮洪, 李萍, 邹汉法¹, 张陆勇

¹中国药科大学现代中药重点实验室、生药学教研室, 南京 210039, 江苏;
²中国科学院大连化学物理研究所国家色谱研究分析中心, 大连 116011, 辽宁

收稿日期:2006-03-03 修回日期:2006-04-07 出版日期:2006-07-26 发布日期:2020-10-30
通讯作者: 李萍, 女, 教授, 博士生导师, 研究方向:生药活性成分与质量评价。Tel:025-85322256 E-mail:lipingli@public1.ptt.js.cn
作者简介:陈丙銮, 女, 博士, 研究方向:生药活性成分与质量评价。Tel:025-85322256 E-mail:peony7629@163.com
基金资助:
国家杰出青年学者科学基金项目(No30325046)

Analysis of biologically active compounds in Erzhi Pills and its compositions by immobilized liposome chromatagraphy

CHEN Bing-luan, SHENG Liang-hong, LI Ping, ZOU Han-fa¹, ZHANG Lu-yong

¹Key Laboratory of Modern TCM, Department of Pharmacognosy, China Pharmaceutical University , Nanjing 210038,Jiangsu, China;
²National Chromatographic R &A Center, Dalian Institute of Chemical Physics, Chinese Academy of Sciences,Dalian 116011, Liaoning, China;

Received:2006-03-03 Revised:2006-04-07 Online:2006-07-26 Published:2020-10-30

摘要/Abstract

摘要： 目的应用固定化脂质体色谱(ILC) 分析中药复方二至丸及其组成药物的经肠吸收成分及质量控制。方法分别观察不同溶剂提取时提取液在ILC色谱柱上的色谱保留与分离, 同时对在ILC 色谱柱上保留的槲皮素及芹菜素进行了定量分析。结果从ILC 色谱柱保留峰的数目和峰面积两方面观察,墨旱莲的75 %乙醇提取优于水提取。二至丸75 %乙醇提取液中槲皮素的含量为9.6 μg·g^-1 , 芹菜素的含量为2.7 μg·g^-1 。墨旱莲提取液中槲皮素的含量为2.8 μg·g^-1 , 芹菜素的含量为3.8 μg·g^-1 。女贞子提取液中槲皮素的含量为9.1 μg·g^-1 , 而芹菜素用ILC 未检测到。结论槲皮素和芹菜素是二至丸中主要的可在ILC 色谱上保留的成分, 其中槲皮素来源于两个组成药物女贞子和墨旱莲, 芹菜素仅来源于墨旱莲。槲皮素和芹菜素可能是二至丸的经肠吸收活性成分。固定化脂质体色谱有望成为中药及其复方肠吸收成分的初筛选和定量评价方法之一。

关键词: 固定化脂质体色谱, 二至丸, 女贞子, 墨旱莲, 经肠吸收成分

Abstract: AIM: To analyse the intestinal absorbable compounds in Erzhi Pills and its compositions by immobilized liposome chromatagraphy (ILC ).METHODS: The ILC column separation and retention of the extracts of Erzhi Pills, Fructus Ligustri Lucidi and Herba Ecliptae extracted with different solvents were observed.Also analysed were the contents of quercetin and apigenin in 75 % ethanol extract of Erzhi Pills, Fructus Ligustri Lucidi and Herba Ecliptae.RESULTS: It was found that the 75 % ethanol extract was better than the water extract based on the number and peak areas of retention peaks.In the 75 % ethanol extract of Erzhi Pills, the content of quercetin was 9.6 μg·g^-1, and the content of the apigenin was 2.7 μg·g^-1.The content of quercetin and apigenin in the 75 % ethanol extract of Herba Ecliptae corresponded to 2.8 and 3.8 μg·g^-1 respectivelly.In the 75 % ethanol extract of Fructus Ligustri Lucidi, the content of quercetin was 9.1 μg·g-1 , but apigenin was not detected.CONCLUSION: Quercetin and apigenin are two main retained components of Erzhi Pills on ILC, the former is contributed by both Fructus Ligustri Lucidi and Herba Ecliptae, the latter only by Herba Ecliptae.Quercetin and apigenin were predicted to be intestinal absorbable components of Erzhi Pills with ILC.Immobilized liposome chromatagraphy can be used as a tool in screening possible bioactive ingredients and in quality control of traditional Chinese medicine (TCM).

Key words: Immobilized liposome chromatagraphy, Erzhi Pills, Fructus Ligustri Lucidi, Herba Ecliptae, main bioactive ingredients

中图分类号:

陈丙銮, 盛亮洪, 李萍, 邹汉法, 张陆勇. 固定化脂质体色谱研究二至丸及其组成药物的经肠吸收成分[J]. 中国临床药理学与治疗学, 2006, 11(7): 776-779.

CHEN Bing-luan, SHENG Liang-hong, LI Ping, ZOU Han-fa, ZHANG Lu-yong. Analysis of biologically active compounds in Erzhi Pills and its compositions by immobilized liposome chromatagraphy[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2006, 11(7): 776-779.

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