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中国临床药理学与治疗学 ›› 2006, Vol. 11 ›› Issue (7): 829-832.

• 研究原著 • 上一篇    下一篇

刺梨提取物CL1 对胃癌SGC-7901 细胞增殖和人脐血CD34+造血干/祖细胞增殖分化能力的影响

刘虹麟1,4, 陈代雄1, 方宁1, 余丽梅1,2, 刘金伟1, 刘祖林1, 文国容 2, 杨小生3, 肖瑜1, 祈莹1, 肖建辉1, 万卫红1, 胡锡阶1   

  1. 1遵义医学院附院贵州省细胞工程重点实验室;
    2药理学教研室, 遵义 563003, 贵州;
    3贵州省中科院天然产物重点实验室, 贵阳 550001, 贵州;
    4遵义医学院病理生理学教研室, 遵义 563003, 贵州
  • 收稿日期:2006-01-24 修回日期:2006-04-05 出版日期:2006-07-26 发布日期:2020-10-30
  • 通讯作者: 陈代雄, , 男, 硕士研究生导师, 教授, 研究方向:干细胞。
  • 作者简介:刘虹麟, 女, 硕士研究生。研究方向:造血干细胞。Tel:13595273107 E-mail:honglin12003@yahoo.com.cn
  • 基金资助:
    贵州省科技厅黔科合计字(2002) 2051 、(2003 ) 3050 课题资助

Effects of Rosa roxburghii Tratt extract-CL1 on gastric carcinoma SGC-7901 cells growth and human umbilical cord CD34+cells proliferation and differentiation

LIU Hong-lin1,4, CHEN Dai-xiong1, FANG Ning1, YU Li-mei1,2, LIU Jin-wei1, LIU Zu-lin1, WEN Guo-rong2, YANG Xiao-sheng3, XIAO Yu1, QI Ying1, XIAO Jian-hui1, WAN Wei-hong1, HU Xi-jie1   

  1. 1Cell Engineering Key Laboratory of Affiliated Hospital;
    2Department of Pharmacology , Zunyi Medical College, Zunyi 563003 , Guizhou, China;
    3 theKey Laboratory of Chemisty for Natural Products of Guizhou Province and Chinese Academy of Scicnces, Guiyang 550001 , Guizhou, China;
    4Department of Pathophysiology , Zunyi Medical College Zunyi 563003 , Guizhou, China
  • Received:2006-01-24 Revised:2006-04-05 Online:2006-07-26 Published:2020-10-30

摘要: 目的 :观察刺梨提取物CL1 对胃癌SGC-7901细胞增殖和人脐血CD34+造血干 祖细胞(HSPC) 增殖分化能力的影响。方法 :采用MTT 法测定活细胞OD 值, 计算CL1 对胃癌SGC-7901 细胞增殖抑制率。免疫磁珠分选人脐血CD34+造血干/祖细胞, 细胞记数、双色直接免疫荧光标记法和流式细胞仪分析细胞增殖分化能力变化。结果 :MTT 检测法显示, 0.01~ 10μmol·L-1的CL1 处理, 剂量依赖性和时间依赖性地抑制胃癌SGC-7901 细胞生长。经14 d 的培养, 与细胞对照组相比, 10 、1 μmol·L-1 的CL1 细胞数有下降趋势, 但差异无显著性;表达粒系/单核巨噬系细胞表型CD15 和CD11b 的细胞百分数无显著差异。经14 d 的培养, 与培养0 d 比较, CD15 显著增高(P<0.05) , CD11b 有增高趋势, 但无显著性差异。结论 :CL1 对胃癌SGC-7901 细胞的体外生长具有抑制作用, 但对人脐带血CD34+HSPC 的增殖、和向粒细胞、单核巨噬细胞分化无明显影响, 表明CL1在抗肿瘤作用剂量下, 不会明显抑制造血干/祖细胞向粒-单细胞的增殖分化, 提示CL1 可能不会引起明显的造血抑制。

关键词: 刺梨, CL1, 胃癌, 造血干 祖细胞, 增殖, 分化

Abstract: AIM: To investigate the effects of Rosa roxburghii Tratt extract-CL1 on proliferation of gastric carcinoma SGC-7901 cells, and on proliferation and differentiation of human umbilical cord (UCB) CD34+cells.METHODS: The OD value of living cells was measured by MTT test.UCB CD34+cells were isolated by magnetic-activated cell sorting, and surface marker of cells was determined using f low cytometry.Cells were counted by trypan blue dye exclusion test.RESULTS: The inhibitory percentages of CL1 (0.01-10 μmol·L-1) on growth of SGC-7901 cells were concentration-dependent and time-dependent from 12 to 96 h.After culturing for 14 d,compared with control group, there was a downtrend of the numbers of CL1 10 and 1 μmol·L-1 but there was no significance(P>0.05) , and there were no difference in the cell number percentage of both expressing CD15 and expressing CD11b.But compared with d 0, the cell number percentage of expressing CD15 were remarkably increased;the cell number percentage of expressing CD11b had an increasing tendency, but it was not significant (P>0.05).CONCLUSION: CL1 has inhibitory effect on growth of SGC-7901 cells in vitro, but does not affect proliferation of CD34+and differentiation of CD34+haematopoietic stem progenitor cells to granulocyte and monocytes.This demonstrates that CL1 has anticancer effect,but does not affect the proliferation and differentiation of haematopoietic stem progenitor cell to granulocytes and monocytes.It may not cause obvious haematopoietic depression.

Key words: Rosa roxburghii Tratt, CL1, gastric carcinoma, heamatopoietic stem progenitor cell, proliferation, differentiation

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