鞘内注射ω-芋螺毒素SO3对神经痛大鼠脊髓Fos 及Jun 蛋白表达的影响

中国临床药理学与治疗学 ›› 2006, Vol. 11 ›› Issue (8): 892-897.

鞘内注射ω-芋螺毒素SO₃对神经痛大鼠脊髓Fos 及Jun 蛋白表达的影响

王红, 周晓巍¹, 张宏, 黄培堂¹

解放军总医院麻醉手术中心, 北京 100853;
¹军事医学科学院生物工程研究所, 北京 100071

收稿日期:2006-04-12 修回日期:2006-07-24 出版日期:2006-08-26 发布日期:2020-11-05
通讯作者: 张宏,女, 博士, 主任医师、教授, 博士导师, 研究方向:临床麻醉、监测及疼痛治疗。Tel:010-66939842 E-mail:zhlina@public.net.cn
作者简介:王红, 女, 博士, 主治医师, 研究方向:临床麻醉及疼痛治疗。Tel:010-66939845 　E-mail:301wh@163.com

Effect of intrathecal ω-conopeptide SO₃ on expression of Fos and Jun protein in spinal cord of rats undergoing neuropathic pain

WANG Hong, ZHOU Xiao-wei¹, ZHANG Hong, HUANG Pei-tang¹

Department of Anesthesiology , General Hospital of PLA, Beijing 100853 , China;
¹Institute of Bioengineering, Academy of Military Medical Science, Beijing 100071 , China

Received:2006-04-12 Revised:2006-07-24 Online:2006-08-26 Published:2020-11-05

摘要/Abstract

摘要： 目的 ω-芋螺毒素SO₃ 是从海洋生物线纹芋螺中提取的一种多肽, 为新型、特异性N 型电压敏感性钙离子通道阻滞剂。通过研究鞘内注射ω-芋螺毒素SO₃ 对坐骨神经慢性挤压伤(CCI 模型) 大鼠脊髓Fos 、Jun 蛋白表达的影响, 探讨N 型钙通道在伤害性信息传递中的作用。方法雄性SD 大鼠72只, 随机均分为9 组, 其中3 组为假手术或单纯制作CCI 模型后3 、14 d;3 组为单次鞘内注射生理盐水或不同剂量SO₃;另3 组为持续鞘内注射生理盐水或不同剂量SO₃ 共7 d 。在预定时间点, 经左心室灌注4%多聚甲醛, 取L4-L6 段脊髓制备石蜡切片。用相应抗体行免疫组织化学染色, 检测各组动物脊髓标本中Fos 样或Jun 样免疫反应阳性细胞的数量。结果大鼠CCI 后脊髓背角Fos 样或Jun 样免疫反应阳性细胞数量显著升高;单次或持续鞘内注射生理盐水对其无明显影响;单次或持续鞘内注射SO₃ 均可剂量依赖性抑制其上升。结论神经损伤后脊髓Fos 、Jun 蛋白表达增加, 鞘内注射SO₃ 可以减轻此种反应, 提示N 型钙通道阻滞剂在脊髓水平可以部分阻断伤害性信息传递。

关键词: ω-芋螺毒素SO₃, 坐骨神经慢性挤压伤, 鞘内注射, N 型钙通道阻滞剂, Fos, Jun

Abstract: AIM: To investigate the effect of intrathecal administration of ω-conopeptide SO₃ , a neotype Ntype calcium channel blocker, on the expression of Fos and Jun protein in the spinal cord of a rat model of chronic constriction injury (CCI).METHODS: 72 male SD rats weighing 230 -270 g were randomly divided into nine groups of 8 animals each.After i.t.NS or SO₃ , the animals were perfused intracardially with 4% paraformaldehyde and the L4 -L6 spinal cord section was removed immediately.The changes of Fos and Jun protein expression in the spinal cord were detected by immunohistochemical techniques.RESULTS: The expression of Fos and Jun protein in the spinal cord was minimal in sham operation grouP;CCI induced significant increase in the expression of Fos and Jun protein in both the ipsilateral and the contralateral spinal cords.Acute or chronic i.t.NS had no effect on these expressions.Both acute or chronic i.t.SO₃ significantly inhibited the increase in Fos and Jun protein expression induced by CCI in a dose-dependent manner.CONCLUSION: Upregulation of expression of Fos and Jun protein may be involved in the development of neuropathic pain, and i.t.SO₃ inhibits the increased expression.

Key words: ω-conopeptide SO₃, chronic constriction injury, intrathecal infusion, N-type calcium channel blocker, Fos, Jun

中图分类号:

R642
R965

王红, 周晓巍, 张宏, 黄培堂. 鞘内注射ω-芋螺毒素SO₃对神经痛大鼠脊髓Fos 及Jun 蛋白表达的影响[J]. 中国临床药理学与治疗学, 2006, 11(8): 892-897.

WANG Hong, ZHOU Xiao-wei, ZHANG Hong, HUANG Pei-tang. Effect of intrathecal ω-conopeptide SO₃ on expression of Fos and Jun protein in spinal cord of rats undergoing neuropathic pain[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2006, 11(8): 892-897.

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鞘内注射ω-芋螺毒素SO₃对神经痛大鼠脊髓Fos 及Jun 蛋白表达的影响

Effect of intrathecal ω-conopeptide SO₃ on expression of Fos and Jun protein in spinal cord of rats undergoing neuropathic pain

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