抗眼镜王蛇毒卵黄抗体的免疫活性及其对眼镜王蛇毒的中和作用

中国临床药理学与治疗学 ›› 2007, Vol. 12 ›› Issue (11): 1250-1254.

抗眼镜王蛇毒卵黄抗体的免疫活性及其对眼镜王蛇毒的中和作用

王桂平^1,2, 刘新艳³, 朱柳³, 余清声³

¹广州医学院护理学院, 广州510182,广东;
²南方医科大学基因研究所,广州510515,广东;
³广州医学院药物研究中心, 广州510180,广东

收稿日期:2007-08-22 修回日期:2007-10-29 发布日期:2020-11-04
通讯作者: 余清声, 男, 教授, 研究方向:抗体类药物开发。E-mail: yqssds@126.com
作者简介:王桂平, 男, 南方医科大学在读博士, 讲师, 研究方向:抗体类药物开发。
基金资助:
广州科技局应用基础研究基金项目(2002J1C0171)

Immunoreactivity and neutralization against king cobra venom of antiking cobra venom IgY

WANG Gui-ping^1,2, LIU Xin-yan³, ZHU Liu³, YU Qing-sheng³

¹Nursing School,Guangzhou Medical College,Guangzhou 510182,Guangdong,China;
²Institute of Gene Research,Southern Medical University,Guangzhou 510515,Guangdong,China;
³Drug Research Center,Guangzhou Medical College,Guangzhou 510180,Guangdong,China

Received:2007-08-22 Revised:2007-10-29 Published:2020-11-04

摘要/Abstract

摘要： 目的: 探讨抗眼镜王蛇毒卵黄抗体的免疫活性及其在体内外对眼镜王蛇毒的中和作用。方法: 采用嗜硫色谱法一步分离纯化抗眼镜王蛇毒卵黄抗体, 通过间接ELISA 法对制备的抗眼镜王蛇毒卵黄抗体进行免疫活性检测, 采用小鼠体内外保护实验评估抗眼镜王蛇毒卵黄抗体对眼镜王蛇毒的中和作用。结果: 嗜硫色谱法制备的卵黄抗体具有良好的免疫活性, 并且对眼镜王蛇毒素显示较好的中和效应, 在体外6 mg/kg 抗眼镜王蛇毒卵黄抗体可有效地中和约4 LD₅₀(约1.6 mg/kg)的眼镜王蛇毒素, 而在体内可有效地中和约3LD₅₀(约1.2 mg/kg)的眼镜王蛇毒素。结论: 抗眼镜王蛇毒卵黄抗体对眼镜王蛇毒具有良好的中和作用, 本项目为抗眼镜王蛇毒卵黄抗体的进一步研究开发提供实验依据。

关键词: 眼镜王蛇毒, 卵黄抗体, 免疫活性, 中和作用

Abstract: AIM: To explore the immunoreactivity and neutralization against king cobra venom of anti-king cobra venom IgY. METHODS: The anti-king cobra venom IgY was developed by thiophilic adsorption chromatography. The biological activity of anti-king cobra venom IgY was detected by indirect ELISA. In vivo and in vitro protective experiments in mice were used for evaluating the neutralization against king cobra venom of anti-king cobra venom IgY. RESULTS: The anti-king cobra venom IgY developed by thiophilic adsorption chromatography showed good biological activity and immunoreactivity,and it also neutralized king cobra venom well. A dose of 6 mg/kg anti-king cobra venom IgY could neutralize 4 LD₅₀(about 1.6 mg/kg)of king cobra venom in vitro,and neutralize 3 LD₅₀(about 1.2 mg/kg)of king cobra venom in vivo. CONCLUSION: The anti-king cobra venom IgY can neutralize king cobra venom,and the present study provides experiment data for further study of antiking cobra venom IgY.

Key words: king cobra venom, egg yolk immunoglobulin, immunoreactivity, neutralization

中图分类号:

R965.2

王桂平, 刘新艳, 朱柳, 余清声. 抗眼镜王蛇毒卵黄抗体的免疫活性及其对眼镜王蛇毒的中和作用[J]. 中国临床药理学与治疗学, 2007, 12(11): 1250-1254.

WANG Gui-ping, LIU Xin-yan, ZHU Liu, YU Qing-sheng. Immunoreactivity and neutralization against king cobra venom of antiking cobra venom IgY[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2007, 12(11): 1250-1254.

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