重组人内抑素对佐剂性关节炎大鼠滑膜组织凋亡相关基因表达的影响

中国临床药理学与治疗学 ›› 2007, Vol. 12 ›› Issue (12): 1359-1363.

重组人内抑素对佐剂性关节炎大鼠滑膜组织凋亡相关基因表达的影响

黄学应^1,2, 陈飞虎¹, 刘永靖^1,3, 张晓明², 袁凤未¹

¹安徽医科大学药学院, ²安徽医科大学人体解剖教研室, ³中国人民解放军105医院胸外科,合肥 230032,安徽

收稿日期:2007-08-17 修回日期:2007-11-23 发布日期:2020-11-10
通讯作者: 陈飞虎,男,博士,教校,博导,研究方向:分子药理和抗炎免疫药理。Tel:0551-5161116 E-mail:dhchina@sohu.com
作者简介:黄学应,男,博士研究生,副教授,硕导,研究方向:分子药理。Tel:0551-5161147 E-mail:hxy229@yahoo.com.cn
基金资助:
国家自然科学基金项目(30572196);安徽省高等学校省级自然科学研究项目(KJ20078034);安徽省博士后科学基金资助项目(07-08)

Effects of recombinant human endostatin on the expression of apoptosisrelated genes in synovial tissue in rats with adjuvant arthritis

HUANG Xue-ying^1,2, CHEN Fei-hu¹, LIU Yong-jing^1,3, ZHANG Xiao-ming², YUAN Feng-lai¹

¹College of Pharmacy, ²Department of Anatomy, Anhui Medical University, Hefei 230032, Anhui, China;
³Department of Candiothoracic Surgery, 105th Hospital of PlA, Hefei 230032, Anhui, China

Received:2007-08-17 Revised:2007-11-23 Published:2020-11-10

摘要/Abstract

摘要： 目的: 观察重组人内抑素(rh-end)对佐剂性关节炎大鼠滑膜组织 p53、fas和 bcl-2 基因表达的影响。方法: 采用SYBR Green I实时荧光定量PCR方法,定量检测该药对佐剂性关节炎大鼠滑膜组织p53、fas和 bcl-2 mRNA表达的影响。结果: 与模型组相比,rh-end治疗组大鼠滑膜组织 fas 和 bcl-2 mRNA表达水平增加,p53 mRNA表达水平降低,差异有统计学意义(P < 0.01)。结论: rh-end可经fas介导诱导佐剂性关节炎大鼠滑膜细胞凋亡,且不依赖于 p53,不能被 bcl-2 所抑制。

关键词: 重组人内抑素, 佐剂性关节炎, 滑膜细胞, 凋亡相关基因

Abstract: AIM: To observe the effects of recombinant human endostatin (rh-end) on the expression of p53, fas and bcl-2 genes in synovial tissue in rats with adjuvant arthritis (AA). METHODS: The effects of rhend on the expression of p53, fas and bcl-2 mRNA in synovial tissue in AA rats were examined quantitatively by SYBR Green I real-time fluor, cent quantitative PCR (FQ-PCR). RESULTS: The expression of fas and bcl-2 mRNA in synovial tissue inprats treated with rh-end was significantly increased, as compared with model con-trols (P < 0.01). However, p53 mRNA expression decreased significantly in rh-end group than that in control (P < 0.01). CONCLUSION: Recombinant human endostatin may induce fas-mediated apoptosis of synovial cells in AA rats, which may be independent on p53 and bcl-2.

Key words: recombinant human endostatin, adjuvant arthritis, synovial cell, apoptosis-related gene

中图分类号:

R965.2

黄学应, 陈飞虎, 刘永靖, 张晓明, 袁凤未. 重组人内抑素对佐剂性关节炎大鼠滑膜组织凋亡相关基因表达的影响[J]. 中国临床药理学与治疗学, 2007, 12(12): 1359-1363.

HUANG Xue-ying, CHEN Fei-hu, LIU Yong-jing, ZHANG Xiao-ming, YUAN Feng-lai. Effects of recombinant human endostatin on the expression of apoptosisrelated genes in synovial tissue in rats with adjuvant arthritis[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2007, 12(12): 1359-1363.

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