木樨草素抗炎作用机制的初步探讨

中国临床药理学与治疗学 ›› 2007, Vol. 12 ›› Issue (2): 195-199.

木樨草素抗炎作用机制的初步探讨

唐宁^1,2, 李子艳¹, 周咏梅¹, 刘保林¹

¹中国药科大学中药药理教研室, 南京 210038, 江苏;
²南京晓庄学院生命科学系, 南京 211171, 江苏

收稿日期:2006-10-12 修回日期:2006-12-12 出版日期:2007-02-26 发布日期:2020-10-27
通讯作者: 刘保林,男, 副教授, 硕士生导师, 研究方向:中药药理。Tel:025-85322009 　E-mail:njlbl55@sohu.com
作者简介:唐宁, 女, 博士, 讲师, 研究方向:中药药理。E-mail:tangningbb@126.com
基金资助:
南京晓庄学院校级重点学科生态学资助项目(2005-2008)

Evaluation of anti-inflammatory effects of luteolin and underlying mechanisems

TANG Ning^1,2, LI Zi-yan¹, ZHOU Yong-mei¹, LIU Bao-lin¹

¹Department of Pharmacology of Chinese MaterialMedica, China Pharmaceutical University, Nanjing 210038, Jiangsu,China;
²Life Science Department, Nanjing Xiaozhuang College, Nanjing 211171, Jiangsu, China

Received:2006-10-12 Revised:2006-12-12 Online:2007-02-26 Published:2020-10-27

摘要/Abstract

摘要： 目的: 初步评价木樨草素的抗炎作用与机制。方法: 采用小鼠足肿胀、肉芽肿和气囊肿模型, 考察木樨草素对急、慢性和渗出性炎症的作用;采用脾淋巴细胞增殖实验, 观察木樨草素对小鼠的免疫反应的影响;通过人全血分析实验, 考察木樨草素对环氧合酶(COX) 活性的影响。结果: 木樨草素对上述三种炎症反应均有明显抑制作用;能够显著抑制刀豆蛋白A(ConA) 刺激引起的小鼠脾细胞增殖, 且呈浓度依赖性;对全血COX-1 活性无明显影响, 但可以显著抑制COX-2 的活性。结论: 木樨草素可以明显抑制急、慢性炎症反应, 其作用机制可能与其对COX-2 活性的抑制有关。

关键词: 木樨草素, 炎症, 环氧合酶

Abstract: AIM: To investigate anti-inflammatory effects of luteolin and underlying mechanisems.METHODS: Three typical inflammatory models, carrageenaninduced paw edema, cotton pellet granuloma and air cyst were used to investigate anti-inflammatory effect of luteolin in vivo.Meanwhile, effects of luteolin on mitogen-induced splenocyte proliferation and cyclooxygenase (COX) activities in vitro were also studied.RESULTS: Luteolin reduced the carrageenan-induced paw edema and effectively inhibited the inflammatory process in the cotton pellet granuloma model.Luteolin significantly inhibited leukocytes influx and reduced elevated levels of PGE2 and 6-keto-PGF1αin the exude induced by carrageenan injection.ConA stimulated splenocyte to proliferate, and the response was effectively suppressed by luteolin.In the whole blood assay, luteolin demonstrated selective inhibition of COX-2 activity.CONCLUSION: Luteolin effectively suppresses inflammatory responses in vivo, and the anti-inflammatory activity is associated with its selective inhibition of COX-2.

Key words: luteolin, inflammation, cyclooxygenase

中图分类号:

R965.2

唐宁, 李子艳, 周咏梅, 刘保林. 木樨草素抗炎作用机制的初步探讨[J]. 中国临床药理学与治疗学, 2007, 12(2): 195-199.

TANG Ning, LI Zi-yan, ZHOU Yong-mei, LIU Bao-lin. Evaluation of anti-inflammatory effects of luteolin and underlying mechanisems[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2007, 12(2): 195-199.

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