转染IL-6基因增强MCF-7人乳腺癌细胞肿瘤相关抗原的表达

摘要/Abstract

摘要： 目的:研究IL-6 影响癌症预后的机制和IL-6 与乳腺癌肿瘤相关抗原CA15-3, CEA 和CA125 的关系。方法:转染外源IL-6 基因到MCF-7 细胞株, 用酶联免疫吸附法(ELISA) 定量培养液中细胞分泌的CA15-3, CEA 和CA125 。结果:在72 h 培养后, 成功转染pCI-neo-IL-6 质粒的MCF-7 细胞分泌IL-6(338.5 ±22.6) pg/10⁶细胞明显高于没有转染的细胞(25.4 ±4.6) pg/10⁶细胞(P <0.01) 和无IL-6 基因的质粒pCI-neo 转染的细胞(19.6 ±3.0) pg/10⁶ 细胞(P <0.01) 。pCI-neo-IL-6 转染的MCF-7 细胞分泌CA15-3, CEA 和CA125 同样明显高于没有转染的细胞和空载体pCI-neo 转染的MCF-7 细胞。特异性IL-6 抗体降低CA15-3, CEA 和CA125 在MCF-7 细胞和IL-6 cDNA 转染的MCF-7 细胞中表达。结论:转染IL-6 基因可增强人乳腺癌细胞肿瘤相关抗原的表达, 高IL-6 血症的乳腺癌病人预后不良部分原因可能是高IL-6 刺激癌细胞表达肿瘤相关抗原。

关键词: 乳腺癌, 肿瘤相关抗原, 白介素-6, 基因转染

Abstract: AIM: To investigate the mechanism by which IL-6 is involved in cancer prognosis, and further to demonstrate the relationship between IL-6 and tumor-associated antigens such as CA15-3, CEA and CA125 in breast cancer.METHODS: In the present study, we transfected an exogenous IL-6 gene into the MCF-7 cells. Secretion of CA15-3, CEA and CA125 into the culture media were measured by Enzyme Linked Immunosorbent Assay (ELISA).RESULTS: After a 72 hours in culture, the amount of IL-6 in the media of pCI-neo-IL-6-transfected MCF-7 cells (338.5 ±22.6 pg/10⁶ cells) was significantly higher than that of non-transfected MCF-7 cells (25.4 ±4.6 pg/10⁶ cells, P <0.01, paired ttest), or pCI-neo-transfected MCF-7 cells (19.6 ± 3.0 pg/10⁶ cells, P <0.01, paired t-test).The levels of CA15-3, CEA and CA125 secreted by the pCI-neo-IL-6-transfected MCF-7 cells were significantly higher than that of the parental MCF-7 cells or pCI-neo-transfected MCF-7 cells.The specific IL-6 antibody could decrease the expression of CA15-3, CEA and CA125 in both the MCF-7 cells and the IL-6 cDNA-transfected MCF-7 cells. CONCLUSION: Transfer of IL-6 gene augments tumorassociated antigens of human breast cancer cells.The association of elevated IL-6 concentration with a poor prognosis in cancer patients may partially be the result of increased expression of tumor-associated antigens by IL-6.

Key words: breast cancer, tumor-associated antigen, interleukin 6, gene transfection

中图分类号:

R965.3

江贤朋, Robert L.Elliott, Jonathan F.Head. 转染IL-6基因增强MCF-7人乳腺癌细胞肿瘤相关抗原的表达[J]. 中国临床药理学与治疗学, 2007, 12(3): 275-281.

JIANG Xian-peng, Robert L.Elliott, Jonathan F.Head. Transfer of interleukin 6 gene into MCF-7 human breast cancer cells enhances expression of tumor-associated antigens[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2007, 12(3): 275-281.

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