人参皂苷Rg2 对拟血管性痴呆大鼠学习记忆的影响

中国临床药理学与治疗学 ›› 2008, Vol. 13 ›› Issue (3): 276-282.

人参皂苷Rg2 对拟血管性痴呆大鼠学习记忆的影响

张荔^1,3, 潘志远¹, 金毅², 龙超良¹, 汪海¹

¹军事医学科学院毒物药物研究所, 北京100850;
²青岛大学医学院, 青岛266012, 山东;
³中国疾控中心全国12320 管理中心, 北京100050

收稿日期:2007-11-30 修回日期:2007-11-30 发布日期:2020-10-15
通讯作者: 汪海, 男, 研究员, 博士生导师, 研究方向:心血管药理学和创新药物研究。Tel:010-66932651 E-mai l:wh @nic.bmi.ac.cn

Effect of ginsenoside-Rg2 on learning and memory of vascular dementiarats

ZHANG Li^1,3, PAN Zhi-yuan¹, JIN Yi², LONG Chao-liang¹, WANG Hai¹

¹Institute of Pharmacology and Toxicology, Academy ofMilitary Medical Science, Beijing 100850, China;
²QingdaoUniversity, Qingdao 266012, Shandong, China;
³Administration Center for National 1230, Beijing 100050, China

Received:2007-11-30 Revised:2007-11-30 Published:2020-10-15

摘要/Abstract

摘要： 目的研究人参皂苷Rg2 对拟血管性痴呆大鼠学习记忆的影响, 并探讨其与谷氨酸离子型受体基因表达的关系。方法双侧颈总动脉永久性结扎制备拟血管性痴呆大鼠模型, 人参皂苷Rg2 治疗后, 检测大鼠自发性活动变化并用水迷宫法检测大鼠学习记忆变化;采用RT-PCR 方法检测海马NR1 、NR2A 、NR2B 和GluR2 基因表达的变化。结果拟血管性痴呆大鼠自发性活动显著减少, 学习记忆能力明显下降, 表现为信息游泳时间和选择游泳时间延长;人参皂苷Rg2 2.5 、5.0 、10.0 mg kg 能剂量依赖性地增加大鼠自发性活动, 缩短信息游泳时间和选择游泳时间, 提高选择正确率, 显著改善大鼠学习记忆障碍。拟血管性痴呆大鼠海马谷氨酸离子型受体亚基NR1 mRNA表达显著增加, 而NR2A 、NR2B 和GluR2 mRNA 的表达均显著降低, 人参皂苷Rg2 能逆转此变化。结论人参皂苷Rg2 能显著改善大鼠学习记忆障碍,机制可能与其有效调节脑内谷氨酸受体亚基基因的表达有关。

关键词: 血管性痴呆, 人参皂苷Rg2, 水迷宫, 学习记忆

Abstract: AIM: To investigate the effect of ginsenoside-Rg2 on learning and memory of vascular dementia rats and its underlying mechanisms, especially the expression of NMDA AMPA receptors. METHODS: Vascular dementia(VD)of rat was induced by permanent occlusion of bilateral common carotid arteries(2OV).The rats were given different doses of ginsenoside-Rg2(2.5, 5.0, 10.0 mg kg)as while as operation. The spontaneous movement and the delayed non-matching-to-position task(DNMPT)in the water maze were used to examine the behavior changes on learning and memory;the mRNA of four different subtypes of NMDA AMPA receptors, including NR1 、 NR2A 、NR2B and GluR2 in hippocampus were determined by the RT-PCR method. RESULTS: The spontaneous movement and spatial learning and memory of VD rats were all decreased.After ginsenoside-Rg2 administration, the spontaneous movement and the spatial learning andmemory were improved;the latency of information swim(IS)and choice swim(CS)decreased, and choice accuration in choice swim increased.The expression of NR1 mRNA was enhanced while NR2A, NR2B and GluR2 were attenuated in hippocampus of VD rats.These pathological changes could be reversed by treatment with ginsenoside-Rg2. CONCLUSION: Ginsenoside-Rg2 can patently improve the spatial learning andmemory of VD rats.The mechanism may be related to the regulation of ginsenoside-Rg2 on the expression of NMDA AMPA receptors in hippocampus.

Key words: vascular dementia, ginsenoside-Rg2, water maze, learning and memory

中图分类号:

R965.2

张荔, 潘志远, 金毅, 龙超良, 汪海. 人参皂苷Rg2 对拟血管性痴呆大鼠学习记忆的影响[J]. 中国临床药理学与治疗学, 2008, 13(3): 276-282.

ZHANG Li, PAN Zhi-yuan, JIN Yi, LONG Chao-liang, WANG Hai. Effect of ginsenoside-Rg2 on learning and memory of vascular dementiarats[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2008, 13(3): 276-282.

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