灯盏乙素抑制过氧化氢诱导PC12 细胞凋亡及机制

中国临床药理学与治疗学 ›› 2008, Vol. 13 ›› Issue (3): 283-287.

灯盏乙素抑制过氧化氢诱导PC12 细胞凋亡及机制

刘萍¹, 洪浩²

¹安徽省安庆市立医院药剂科, 安庆246003, 安徽;
²中国药科大学药理教研室, 南京210009, 江苏

收稿日期:2008-02-13 修回日期:2008-02-13 发布日期:2020-10-15
通讯作者: 洪浩,, 男, 博士, 副教授, 研究方向:神经与内分泌药理。Tel:025-83207491 E-mai l:haohongchina @hotmail.com

Scutellarin attenuates hydrogen peroxide-induced apoptosis in PC12 cells

LIU Ping¹, HONG Hao²

¹Department of Pharmacy, Anqing MunicipaL Hospital, Anqing 246003, Anhui, China;
²Department of Pharmacology, China Pharmaceutical University, Nanjing 210009, Jiangsu, China

Received:2008-02-13 Revised:2008-02-13 Published:2020-10-15

摘要/Abstract

摘要： 目的观察灯盏乙素对过氧化氢(H2O2) 诱导PC12 细胞凋亡的抑制作用及其作用机制。方法采用H2O2 诱导PC12 细胞凋亡模型, 用碘化丙啶(PI) 单染和PI Annexin V 双染检测细胞凋亡, 用DNA 琼脂糖凝胶电泳观察DNA 断裂, 并采用RTPCR检测Bcl-2 mRNA 表达、荧光光度法检测caspase-3 活性。结果灯盏乙素可以抑制H2O2 诱导的膜磷脂酰丝氨酸外翻, 增加Bcl-2 mRNA 表达, 减小caspase-3 活性, 并降低DNA 片段化和细胞凋亡率。结论灯盏乙素显著抑制H2O2 诱导的细胞凋亡, 其抗细胞凋亡作用可能与其增加Bcl-2表达、抑制caspase-3 活化有关。

关键词: 灯盏乙素, PC12 细胞, 过氧化氢, 细胞凋亡, Bcl-2, caspase-3

Abstract: AIM: To investigate the mechanisms of inhibition of hydrogen peroxide (H2O2)-induced cell apoptosis in PC12 cells by scutellarin. METHODS: Exposure of cells to hydrogen peroxide (H2O2), a inducer of oxidative stress, triggered a typical apoptosis determined by PI singel staining, Annexin V-PI double staining and DNA agarose gel electrophoresis.Bcl-2 mRNA expression was determined by RT-PCR and caspase-3 was detected by fluoremetry. RESULTS: Scutellarin significantly inhibited H2O2-induced the DNA fragmentation and the externalization of phosphatidylserine(PS), and decreased the percentage of cell apoptosis induced by H2O2 in PC12 cells.Also, down-regulation of Bcl-2 expression and up-regulation of caspase-3 activity resulting from H2O2 exposure were significantly reduced by scutellarin in PC12 cells. CONCLUSION: Scutellarin could significantly protect cell against H2O2-induced apoptosis, which might be associated with the increase of Bcl-2 expression and the inhibition of caspase-3 activity.

Key words: scutellarin, PC12 cells, hydrogen peroxide, apoptosis, Bcl-2, caspase-3

中图分类号:

R965.2

刘萍, 洪浩. 灯盏乙素抑制过氧化氢诱导PC12 细胞凋亡及机制[J]. 中国临床药理学与治疗学, 2008, 13(3): 283-287.

LIU Ping, HONG Hao. Scutellarin attenuates hydrogen peroxide-induced apoptosis in PC12 cells[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2008, 13(3): 283-287.

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