生物被膜肺炎克雷伯杆菌AmpC酶、超广谱β-内酰胺酶的检测

中国临床药理学与治疗学 ›› 2008, Vol. 13 ›› Issue (4): 384-387.

生物被膜肺炎克雷伯杆菌AmpC酶、超广谱β-内酰胺酶的检测

李乃静¹, 李岩¹, 潘作东¹, 何平¹, 李胜岐²

¹中国医科大学附属盛京医院老年病科,²呼吸科,沈阳110004,辽宁

收稿日期:2008-01-15 修回日期:2008-03-24 出版日期:2008-04-26 发布日期:2020-10-12
通讯作者: 何平,男,教授,博士生导师,主要从事老年感染性疾病研究。 E-mail:hepcmu@163.com
作者简介:李乃静,女,博士,副教授,主要从事老年感染性疾病研究。 E-mail:lnjw2003@yahoo.com.cn
基金资助:
辽宁省自然科学基金资助项目(20052083)

Determination of the AmpC and ESBL produced in the biofilm of klebsiella pneumoniae

LI Nai-jing¹, LI Yan¹, PAN Zuo-dong¹, HE Pin¹g, LI Sheng-qi²

¹ Department of Senile Disease,² Department of Respiratory Disease, Afilited Shengjing Hospital, China Madical University, Shenyang l10004,Liaoning,China

Received:2008-01-15 Revised:2008-03-24 Online:2008-04-26 Published:2020-10-12

摘要/Abstract

摘要： 目的: 探讨生物被膜(BF)菌的耐药机制,为临床合理应用抗生素提供理论依据。方法: 应用改进的平板培养法建立肺炎克雷伯杆菌BF模型,用银染法和扫描电镜观察鉴定。采用改良三维试验法检测超广谱β-内酰胺酶(ESBIs)及AmpC酶。结果: 浮游肺炎克雷伯杆菌单产AmpC酶,单产ESBLs酶及同时产ESBLs和AmpC酶的菌株分别为2.5%(1/40)、20.0%(8/40)、2.5%(1/40);BF肺炎克雷伯杆菌单产AmpC酶,单产ESBLs及同时产ESBIs和AmpC酶的菌株分别为20.0%(8/40)、45.0%(18/40)、22.5%(9/40)。对浮游组和BF组的检出率两两分别进行x²检验,BF组各酶的检出率均明显高于普通浮游组各酶的检出率(P<0.05)。产酶BF肺炎克雷伯杆菌对8种抗生素(除亚胺培南全部敏感外)的耐药率均较高。结论: BF的形成和产生ESBL及AmpC酶的协同作用是肺炎克雷伯杆菌耐药的主要原因之一。

关键词: 肺炎克雷伯杆菌, 生物被膜, 超广谱β-内酰胺酶, AmpC酶

Abstract: AIM: To evaluate the drug fast mecha-nism of bacteria gowing in biofilm，and to provide the theoretical basis for selecting antimicrobial agents in clinic. METHODS: The model of klebsiella pneu-moniae bacterial biofilm were built up with the modifed flat-board method and identified with the method stain-ing with AgNO₃ and confocal scanning laser microscopy. The ESBLs and AmpC were detected by improved three dimensional test. RESULTS: The detection rates of AmpC，ESBLs and AmpC plus ESBLs in isolated klebsiella pneumoniae were 2.5%(1/40)，20.0%(8/40) and 2.5%(1/40),whereas the detection rates of AmpC，ESBLs and AmpC plus ESBLs in biofilm kleb-siella pneumoniae were 20.0%(840)，45.0%(18/40)and 22.5%(940). The resistance rates of the biofilm klebsiella pneumoniae producting AmpC and ESBLs to eight kinds of antibiotics were higher. CONCLUSION: The synergetic effect of the formation of biofilm and the pioduction of ESBLs and AmpC is one of the main reasons that klebsiella pneumoniae resists antibiotics.

Key words: klebsiella pneumoniae, biofilm, ESBL, AmpC

中图分类号:

R378

李乃静, 李岩, 潘作东, 何平, 李胜岐. 生物被膜肺炎克雷伯杆菌AmpC酶、超广谱β-内酰胺酶的检测[J]. 中国临床药理学与治疗学, 2008, 13(4): 384-387.

LI Nai-jing, LI Yan, PAN Zuo-dong, HE Ping, LI Sheng-qi. Determination of the AmpC and ESBL produced in the biofilm of klebsiella pneumoniae[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2008, 13(4): 384-387.

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