木犀草素减轻氧化应激引起的血管内皮损伤

中国临床药理学与治疗学 ›› 2008, Vol. 13 ›› Issue (4): 418-424.

木犀草素减轻氧化应激引起的血管内皮损伤

潘芳芳, 周咏梅, 张敏, 宋隽娜, 刘保林

中国药科大学中药药理教研室,南京210038,江苏,中国

收稿日期:2008-01-28 修回日期:2008-04-02 出版日期:2008-04-26 发布日期:2020-10-12

Luteolin ameliorates endothelial dysfunction induced by oxidative stress

PAN Fang-fang, ZHOU Yong-mei, ZHANG Min, SONG Jun-na, LIU Bao-lin

Department of Pharmacology of Chinese Materia Medica,China Pharmaceutical University , Nanjing 210038 , Jiangsu, China

Received:2008-01-28 Revised:2008-04-02 Online:2008-04-26 Published:2020-10-12
Contact: LIU Bao-lin, male, professor, the tutor of post-graduate student, majoring in biochem-pharmacology Tel:025-85322009 　E-mail:njlbl55@sohu.com

摘要/Abstract

摘要： 目的: 探讨木犀草素对叔丁基过氧化氢致血管内皮损伤的保护作用及相关机制。方法: 首先通过制备大鼠胸主动脉环, 观察木犀草素对叔丁基过氧化氢所致血管张力变化的影响;再采用叔丁基过氧化氢诱导血管内皮细胞氧化损伤模型, 观察木犀草素对其细胞形态学变化及细胞活力的影响, 并用 RT-PCR 检测 eNOS 和COX-1 mR-NA 的含量变化。结果: 木犀草素能够浓度依赖性地对抗叔丁基过氧化氢导致的血管舒张功能损伤及细胞损伤作用, 且浓度依赖性地减弱叔丁基过氧化氢对内皮细胞eNOS mRNA 表达抑制的影响。结论: 木犀草素是一种有效的舒血管物质, 它可以起到抗氧化的作用, 减轻氧化应激反应, 并可能通过维持eNOS 活性等血管内皮途径舒张血管。

关键词: 木犀草素, 主动脉环, 内皮细胞, 叔丁基过氧化氢

Abstract: AIM: To evaluate the protective effect of luteolin on endothelial dysfunction induced by tert-butyl hydro-peroxide ( t-BOOH ) .METHODS: We observed the effect of luteolin on t-BOOH-induced contractions in the aorta rings with or without endothelium, which were incubated with luteolin ( 10^-6 to 10^-4mol/L) for 30 min before determining the concentra-tion-response to t-BOOH .Cultured endothelial cell line ( ECV304) was pretreated with different concentrations of luteolin (10^-6 to 10^-4mol/L)for 30 min and hen exposed to 10^-5mol/L t-BOOH for 24 hours .Cellmorphology was observed, and cell viability was deter-mined by MTT assay .Meanwhile, RT-PCR was used to measure the expression of eNOS and COX-1 .RESULTS: Increasing concentrations of t-BOOH pro-duced concentration-dependent contractions in aorta rings isolated from rats, luteolin effectively attenuated the contraction in a concentration-dependent manner, and the relaxation response was greater in intact endo-thelium segments .In MTT and RT-PCR assays, luteo-lin effectively reduced the cytotoxicity of t-BOOH to endothelium cell and increased the expression of nitric oxide synthase ( eNOS ) mRNA, which was greatly down-regulated by t-BOOH.CONCLUSION: Luteo-lin effectively protects the endothelium from the impairment of oxidative stress, and the protection could be related to its negative modulation towards t-BOOH-in-duced contractions in the aorta .

Key words: luteolin, aorta ring, endothelial cell, t-BOOH

中图分类号:

R965.2

潘芳芳, 周咏梅, 张敏, 宋隽娜, 刘保林. 木犀草素减轻氧化应激引起的血管内皮损伤[J]. 中国临床药理学与治疗学, 2008, 13(4): 418-424.

PAN Fang-fang, ZHOU Yong-mei, ZHANG Min, SONG Jun-na, LIU Bao-lin. Luteolin ameliorates endothelial dysfunction induced by oxidative stress[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2008, 13(4): 418-424.

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