雌激素对卵巢切除后雌性大鼠心肌组织中雌激素受体表达的影响

中国临床药理学与治疗学 ›› 2008, Vol. 13 ›› Issue (4): 431-434.

雌激素对卵巢切除后雌性大鼠心肌组织中雌激素受体表达的影响

钟晓华, 王卉, 李庆平

南京医科大学药理学系,南京210029,江苏

收稿日期:2008-03-03 修回日期:2008-04-09 出版日期:2008-04-26 发布日期:2020-10-12
通讯作者: 李庆平,女,博士,教授,硕士生导师,研究方向:心血管药理。Tel:025-86862883 E-mail:qingpingli@yahoo.com.cn
作者简介:钟晓华,女,硕士,研究方向:心血管药理。Tel:025-86862883 E-mail:xiaohuazhong066600@126.com

Effects of estrogen on estrogen receptors in myocardium of ovariecto-mized female rats

ZHONG Xiao-hua, WANG Hui, LI Qing-ping

Department of Pharmacology , Nanjing Medical University , Nanjing 210029 , Jiangsu, China

Received:2008-03-03 Revised:2008-04-09 Online:2008-04-26 Published:2020-10-12

摘要/Abstract

摘要： 目的: 探讨雌激素对卵巢切除后雌性大鼠心肌组织中雌激素受体表达的影响。方法: 雌性SD大鼠卵巢切除术(OVX)后1周, 随机分成3组:OVX+雌激素(0.15mg/kg,s.c.)、OVX+生理盐水、对照组。4 周后,Western blotting检测α和β两种雌激素受体在心肌组织中表达。结果: 卵巢切除后,血清雌激素水平与对照组[(88±22 vs 403±59)pmol/L,P<0.05]相比明显下降,左心室肌中两种雌激素受体表达均下降( P<0.05)。给予雌激素后,血清雌激素水平上升为(3864±105)pmol/L,雌激素β受体表达增多(P<0.05),α受体表达下降(P<0.05)。结论: 雌激素改变卵巢切除后雌性大鼠心肌组织中雌激素受体的表达。

关键词: 雌激素受体, 心肌组织, 卵巢切除

Abstract: AIM: To investigate the expression of estrogen receptors in myocardium of ovariectomized fe-male rats.METHODS: One week after bilateral ovariectomy, the female SD rats were divided into 3 groups randomly :OVX +estrogen (0.15 mg/kg,s.c.) , OVX +normal sodium and control .After 4 weeks of treatment, the expression of estrogen receptors in the myocardium were evaluated by Western blotting.RESULTS: After ovariectomy, serum estrogen de-creased significantly, compared with control[( 88±22 vs 403±59) pmol/L, P <0 .05], the expression of estrogen receptors decreased( P<0.05) .After estrogen replacement therapy, serum estrogen increased [( 3864±105) pmol/L], estrogen upregulated the ex-pression of ERβreceptor( P<0.05) and downregulat-ed ERαreceptor (P<0.05) in myocardium .CONCLUSION: Estrogen replacement modifies the expres-sion of estrogen receptors in myocardium of ovariecto-mized female rats.

Key words: estrogen receptor, myocardium, ovariectomy

中图分类号:

R965.2

钟晓华, 王卉, 李庆平. 雌激素对卵巢切除后雌性大鼠心肌组织中雌激素受体表达的影响[J]. 中国临床药理学与治疗学, 2008, 13(4): 431-434.

ZHONG Xiao-hua, WANG Hui, LI Qing-ping. Effects of estrogen on estrogen receptors in myocardium of ovariecto-mized female rats[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2008, 13(4): 431-434.

参考文献

[1] Aurigemma GP,Gaasch WH.Gender differences in older patients with pressure-overload hypertrophy of the left ven-tricle[J].Cardiology,1995,86(4):310-317.
[2] Writing Group for theWomen's Health Initiative Investiga-tors.Risks and benefits of estrogen plus progestin in healthy postmenopausal women.Principal results from the-Women's Health Initiative randomized controlled trial[J]. JAMA,2002,288(3):321-333.
[3] Simon JA,Hunninghake DB,Agarwal SK,et al.Effect of estrogen plus progestin on risk for biliary tract surgery in postmenopausal women with coronary artery disease:The Heart and Estrogen progestin Replacement Study[J].Ann Int Med,2001,135(7):493-501.
[4] Manson JE,Hsia J,Johnson KC,et al.Estrogen plus progestin and the risk of coronary heart disease[J].N En-gl J Med,2003,349(6):523-534.
[5] Hall JM,McDonnell DP.The estrogen receptor {beta}-isoform (ER{beta}) of the human estrogen receptor modu-lates ER{alpha}transcriptional activity and is a key regula-tor of the cellular response to estrogens and antiestrogens[J].Endocrinology,1999,140(12):5566-5578.
[6] Carley ME,Rickard DJ,Gebhar JB,et al.Distribution of estrogen receptors αand βmRNA in mouse urogenital tis-sues and their expression after oophorectomy and estrogen replacement[J].Int Urogynecol J,2003,14(2):141-145.
[7] Zhang JQ ,Cai WQ,Zhou D,et al.Distribution and dif-ferences of estrogen receptor betaimmunoreactivity in the brain of adult male and female rats[J].Brain Res,2002,935(1 2):73-80.
[8] Jin M ,Jin F,Zhang L,et al.Two estrogen replacement therapies differentially regulate expression of estrogen re-ceptors alpha and beta in the hippocampus and cortex of ovariectomized rat[J].Brain Res Mol Brain Res,2005,142(2):107-114.
[9] Ropero AB,Eghbali M,Minosyan TY,et al.Heart estro-gen receptor alpha:Distinct membrane and nuclear distri-bution patterns and regulation by estrogen[J].J Mol Cell Cardiol,2006,41(3):496-510.
[10] Saceda M ,Lindsey RK ,Solomon H,et al.Estradiol reg-ulates estrogen receptor mRNA stability[J].J Steroid Bio-chem Mol Biol,1998,66(3):113-120.
[11] Thakur MK,Sharma PK.Transcription of estrogen receptor αand βin mouse cerebral cortex:Effect of age,sex,17b-estradiol and testosterone[J].Neurochem Int,2007,50(2):314-321.
[12] Xu Y,Arenas IA,Armstrong SJ,et al.Estrogen modula-tion of left ventricular remodeling in the aged heart[J]. Cardiovasc Res,2003,57(2):388-394.
[13] Zhu Y,Bian Z,Lu P,et al.Abnormal vascular function and hypertension in mice deficient in estrogen receptorβ[J].Science,2001,295(5554):505-508.
[14] Morales DE,McGowan KA,Grant DS,et al.Estrogen promotes angiogenic activity in human umbilical vein endo-thelial cells in vitro and in a murine model[J].Circula-tion,1995,91(3):755-763.
[15] Losordo DW,Kearney M ,Kim EA,et al.Variable ex-pression of the estrogen receptor in normal and atheroscle-rotic coronary arteries of premenopausal women[J].Circu-lation,1994,89(4):1501-1510.
[16] Post WS,Goldschmidt-Clermont PJ,Wilhide CC,et al. Methylation of the estrogen receptor gene is associated with aging and atherosclerosis in the cardiovascular system[J]. Cardiovasc Res,1999,43(4):985-991.