槲寄生通过抑制NF-κB活性来诱导人大肠癌HCT116细胞凋亡

中国临床药理学与治疗学 ›› 2008, Vol. 13 ›› Issue (7): 730-734.

槲寄生通过抑制NF-κB活性来诱导人大肠癌HCT116细胞凋亡

林蕾¹, 叶孟², 王少敏², 倪曙民², 吴骁²

¹宁波大学医学院附属医院皮肤科, ²肿瘤科, 宁波 315020, 浙江

收稿日期:2007-07-05 修回日期:2008-04-09 出版日期:2008-07-26 发布日期:2020-10-14
通讯作者: 叶孟,男,博士,副教授,研究方向:肿瘤化疗和肿瘤信号转导途径。Tel:0574-66892008 E-mail:dryemeng@yahoo.com.cn
作者简介:林蕾,女,副主任医师,研究方向:皮肤肿瘤及基础。Tel:0574-81182808 E-mail:drll606@yahoo.com.cn
基金资助:
宁波市博士基金资助项目(2005A610020);宁波市医学科研项目(200529)

Mistletoe induces apoptosis by down-regulating the constitutive activation of nuclear factor-κB in HCT116 cells

LIN Lei¹, YE Meng², WANG Shao-ming², NI Shu-min², WU Xiao²

¹Department of Dermatology, ²Department of Oncology, Affiliated Hospital of Medical College of Ningbo University, Ningbo 315020, Zhejiang, China

Received:2007-07-05 Revised:2008-04-09 Online:2008-07-26 Published:2020-10-14

摘要/Abstract

摘要： 目的:探讨槲寄生诱导人大肠癌HCT116细胞凋亡所涉及的信号转导途径。方法:采用MTT法测定细胞的增殖活力,琼脂糖凝胶电泳观察细胞调亡,流式细胞仪进行细胞周期分析,Westernblotting和DIG-EMSA研究细胞调亡途径。结果:槲寄生对HCT116细胞有明显的生长抑制作用,琼脂糖凝胶电泳检测到典型的DNA梯状条带;槲寄生可通过抑制IκBα蛋白磷酸化进而抑制NF-κB;槲寄生可增强羟基喜树碱对HCT116细胞诱导的凋亡作用。结论:槲寄生对HCT116细胞有明显的生长抑制和诱导调亡作用,并且通过抑制NF-κB活性来诱导人大肠癌HCT116细胞凋亡。

关键词: 槲寄生, 大肠癌HCT116细胞系, 核转录因子κB, 凋亡

Abstract: AIM: To investigate the role of NF-κB in apoptosis induced by mistletoe in human colon cancer HCT116 cells.METHODS: The cell growth inhibiting was measured by MTT assay, the cell apoptosis was observed by the agarose gel electrophoresis.The protein expression was detected by Western Blotting. DIG-EMSA was conducted to detemine the DNA-binding activation of NF-κB.RESULTS: Mistletoe had a remarkable growth inhibitory action on HCT116 cells. After exposed to mistletoe, HCT116 cells presented some morphologic features of apoptosis including cell shrinkage, nuclear condensation and DNA fragmentation.NF-κB was inhibited in cancer cells treated with mistletoe.Mistletoe may potentiate the cytotoxic effects of chemotherapeutic agents such as HCPT.CONCLUSION: Mistletoe may induce apoptosis in HCT116 cell line.Inhibitory action of NF-κB may play a significant role in apoptosis induced by mistletoe.

Key words: mistletoe, HCT116 cell line, NF-κB, apoptosis

中图分类号:

R363

林蕾, 叶孟, 王少敏, 倪曙民, 吴骁. 槲寄生通过抑制NF-κB活性来诱导人大肠癌HCT116细胞凋亡[J]. 中国临床药理学与治疗学, 2008, 13(7): 730-734.

LIN Lei, YE Meng, WANG Shao-ming, NI Shu-min, WU Xiao. Mistletoe induces apoptosis by down-regulating the constitutive activation of nuclear factor-κB in HCT116 cells[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2008, 13(7): 730-734.

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