Cpd5 对人卵巢癌SKOV3 细胞增殖抑制和凋亡诱导作用

中国临床药理学与治疗学 ›› 2009, Vol. 14 ›› Issue (10): 1121-1127.

Cpd5 对人卵巢癌SKOV3 细胞增殖抑制和凋亡诱导作用

陈莉¹, 霍冠华², 吕耀凤^1,3, 张芹²

¹滨州医学院妇产科学教研室, ²滨州医学院组织胚胎学教研室, 滨州 256603, 山东

收稿日期:2009-07-29 修回日期:2009-09-24 发布日期:2020-10-29
通讯作者: 吕耀凤,女,教授,研究方向:妇科肿瘤学。E-mail:lyfhyq@hotmail.com
作者简介:陈莉,女,硕士研究生,研究方向:妇科肿瘤。Tel:0543-3258729 E-mail:cl830104@126.com
基金资助:
山东省卫生厅(2001CA1CFA1);教育厅科技计划项目(J01K12)

Proliferation inhibition and apoptosis induction in human ovarian cancer SKOV3 cells by Cpd5

CHEN Li¹, HUO Guan-hua², LV Yao-feng^1,3, ZHANG Qin²

¹Department of Obstetrics and Gynecology, the Affiliated Hospital of Binzhou Medical College, 2Department of Histology & Embryology, Binzhou Medical College, Binzhou 256603, Shandong, China;

Received:2009-07-29 Revised:2009-09-24 Published:2020-10-29

摘要/Abstract

摘要： 目的观察Cpd5[2-(2-巯基乙醇) -3-甲基-1, 4-萘醌, Compound 5] 对人卵巢癌SKOV3 细胞增殖和凋亡的影响。方法 MTT 法观察Cpd5 对SKOV3细胞的生长抑制作用, Annexin V PI 双标记流式细胞术检测Cpd5 对SKOV3 细胞的凋亡诱导,Hoechst-33258 染色荧光显微镜观察细胞凋亡形态。结果 5 、10 、20 、30 、40 、50 、60 μmol/LCpd5 处理SKOV3 细胞48 h, 生长抑制率分别为2.77 %、5.19 %、10.61 %、41.15 %、71.37 %、82.90 %、89.81 %。不同时间点(12 、24 、48 和72 h) 检测, 细胞生长抑制率存在剂量-时间依赖关系。流式细胞术检测, 30 μmol/LCpd5 处理12 、24 和48 h 后,细胞凋亡率分别达9.25 %、20.07 %、56.16 %;50 μmol/L组的细胞凋亡率明显高于30 μmol/L,且随时间增加而显著增加。用40 、50 和60 μmol/LCpd5 处理细胞12 h, 光镜观察即可见明显的形态学改变。荧光显微镜观察:Annexin VEGFPPI 双染显示, 实验组比阴性对照组细胞凋亡率明显增多;Cpd5 作用24 、48 h 后, Hoechst-33258 染色可见细胞出现明显的凋亡形态,20 μmol/L浓度组凋亡率增加, 30 、40 、50 、60 μmol/L各组细胞凋亡率显著增加。结论 Cpd5能以剂量-时间依赖的方式抑制SKOV3 细胞增殖并诱导凋亡, 是潜在的抗卵巢癌新化合物。

关键词: Cpd5, 细胞凋亡, 卵巢癌, SKOV3

Abstract: AIM: To investigate the effect of Cpd5 on the proliferation inhibition and apoptosis induction in human ovarian cancer SKOV3 cells. METHODS: The growth inhibition of Cpd5 in SKOV3 cells was detected by MTT assay.AnnexinV PI staining was employed for quantifying apoptotic cells by fluorescence microscopy and flow cytometry.The apoptotic morphology was observed by Hoechst-33258 staining. RESULTS: After Cpd5 treatment at the concentrations of 5, 10, 20, 30, 40, 50 and 60 μmol/Lfor 48 h, the ratios of the cells growth inhibition were 2.77 %, 5.19 %, 10.61 %, 41.15 %, 71.37 %, 82.90 % and 89.81 %, respectively.The proliferation was inhibited after Cpd5 treatment at different times of 12, 24, 48 and 72 h, the cells were inhibited by Cpd5, in a dose-time-dependent manner.The apoptotic cells accounted for 9.25 %, 20.07 % and 56.16 %, after Cpd5 treatment by 30 μmol/Lfor 12, 24 and 48 h, respectively.The ratio of apoptotic cells in 50 μmol/Lgroup was significantly higher than that in 30 μmol/Lgroup.The morphological changes were emerged after Cpd5 treatment at the concent rations of 40, 50 and 60 μmol/Lfor 12 h.Compared with the control group, the ratios of the apoptotic cells were increased significantly in the groups induced by Cpd5 at different concentrations.The apoptotic cells were increased in the groups of Cpd5 treatment at 20 μmol/Lfor 24 h and 48 h by Hoechst-33258 staining.Furthermore, the ratios of apoptotic cells were increased significantly in the groups of Cpd5 treatment at 30, 40, 50 and 60 μmol/LCpd5 at different time-points. CONCLUSION: Cpd5 can induce proliferation inhibition and apoptosis in SKOV3 cells, in a dose-time-dependent manner.Our data reveal that Cpd5 is a novel anti-ovarian cancer compound.

Key words: Cpd5, apoptosis, ovarian cancer, SKOV3

中图分类号:

R965.2

陈莉, 霍冠华, 吕耀凤, 张芹. Cpd5 对人卵巢癌SKOV3 细胞增殖抑制和凋亡诱导作用[J]. 中国临床药理学与治疗学, 2009, 14(10): 1121-1127.

CHEN Li, HUO Guan-hua, LV Yao-feng, ZHANG Qin. Proliferation inhibition and apoptosis induction in human ovarian cancer SKOV3 cells by Cpd5[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2009, 14(10): 1121-1127.

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