红霉素对哮喘大鼠磷酸化P38 及Th2 类细胞因子的影响

中国临床药理学与治疗学 ›› 2009, Vol. 14 ›› Issue (10): 1151-1155.

红霉素对哮喘大鼠磷酸化P38 及Th2 类细胞因子的影响

张近波¹, 吴成云², 李智强³, 戴元荣²

¹温州医学院附属温岭医院EICU, 温岭 317500, 浙江;
²温州医学院附属第二医院呼吸内科, ³温州医学院附属第一医院ICU, 温州 325000, 浙江

收稿日期:2009-04-23 修回日期:2009-06-15 发布日期:2020-10-29
通讯作者: 吴成云,男,硕士,副主任医师,主要从事慢性阻塞性肺疾病、支气管哮喘方面的研究。E-mail:wcyzxqdd@hotmail.com
作者简介:张近波,男,硕士研究生,医师,主要从事危重病方面的研究。Tel:13757728837 E-mail:zhangjinbo0661 @sina.com
基金资助:
温州市科学技术局资助项目(Y2006A036)

Effects of erythromycin on the expressions of P38 and Th2 Cytokines in rats with asthma

ZHANG Jin-bo¹, WU Cheng-yun², LI Zhi-qiang³, DAI Yuan-rong²

¹Department of EICU, the Wenling Hospital Affiliated to Wenzhou Medical College, Wenling 317500, Zhejiang, China;
2Department of Respiratory Medicine, the Second Affiliated Hospital Wenzhou Medical College, Wenzhou 325000, Zhejiang, China;
3Department of ICU, the First Affiliated Hospital Wenzhou Medical College, Wenzhou 325000, Zhejiang, China

Received:2009-04-23 Revised:2009-06-15 Published:2020-10-29

摘要/Abstract

摘要： 目的探讨红霉素对哮喘大鼠磷酸化P38及Th2 类细胞因子的影响。方法分离培养正常对照组、哮喘组、红霉素处理组大鼠的外周血单核细胞(PBMCs)。分别采用ELISA 方法检测细胞上清液中IL-4 、IL-5 、IL-13 浓度。逆转录聚合酶链反应(RT-PCR) 、Western blotting 技术检测PBMCs P38mRNA 、P38 磷酸化蛋白的表达。结果哮喘组PBMCsP38mRNA 、P38 磷酸化蛋白表达水平及细胞上清液中IL-4 、IL-5 、IL-13 的浓度较正常对照组显著升高(P <0.05), 红霉素处理组P38 mRNA 、磷酸化蛋白表达水平及细胞上清液中IL-4 、IL-5 、IL-13 的浓度较哮喘组显著降低(P <0.05)。通过直线相关分析发现, PBMCs P38 磷酸化蛋白与细胞上清液中IL-4 、IL-5 、IL-13 的表达水平之间均呈显著正相关(r 分别=0.75 、0.82 、0.70, P 均<0.05)。结论 P38 可能参与支气管哮喘的病理生理过程。红霉素可能通过作用于P38 这一靶点发挥其免疫调节作用。

关键词: 哮喘, 外周血单个核细胞, P38, 红霉素

Abstract: AIM: The purpose of this study is to explore the effects of erythromycin on the expressions of phosphorylation P38 and Th2 cytokines in asthmatic rats. METHODS: Thirty male Sprague-Dawley adult rats were randomly divided into normal, asthma and erythromycin-treated group (n =10 for each group). Peripheral blood monouclear cells(PBMCs) of rats were collected and cultured in each group.The concentrations of IL-4, IL-5 and IL-13 in cell supernatant were detected by ELISA.The mRNA and phosphor protein of P38 in PBMCs were detected by reverse transcription polymerase chain reaction (RT-PCR) and Western blotting (WB), respectively. RESULTS: The expression of mRNA and phosphor protein of P38 in PBMCs and the concentrations of IL-4, IL-5 and IL-13 in cell supernatant were higher in asthmatic group than those in normal group (P <0.05).Compared with the asthmatic group, the above indexes were significantly decreased in erythromycin-treated group (P <0.05). There were positive correlations between the expression of phosphor-P38 in PBMCs and the concentrations of IL-4, IL-5 and IL-13 in cell supernatant (r =0.75, 0.82 and 0.70, all P <0.05). CONCLUSION: The P38 may be involved in the pathogenesis of bronchial asthma.Erythromycin playing its role in the treatment of asthma may be achieved by acting on the P38.

Key words: asthma, peripheral blood mononuclear cell, P38, erythromycin

中图分类号:

R965.2

张近波, 吴成云, 李智强, 戴元荣. 红霉素对哮喘大鼠磷酸化P38 及Th2 类细胞因子的影响[J]. 中国临床药理学与治疗学, 2009, 14(10): 1151-1155.

ZHANG Jin-bo, WU Cheng-yun, LI Zhi-qiang, DAI Yuan-rong. Effects of erythromycin on the expressions of P38 and Th2 Cytokines in rats with asthma[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2009, 14(10): 1151-1155.

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