缺血性损伤对肝移植胆道微循环的影响及重组人生长激素的保护作用

中国临床药理学与治疗学 ›› 2009, Vol. 14 ›› Issue (11): 1221-1224.

缺血性损伤对肝移植胆道微循环的影响及重组人生长激素的保护作用

王峥, 周杰, 林艺雄, 崔忠林, 张起帆

南方医科大学附属南方医院肝胆外科, 广州510515, 广东

收稿日期:2009-08-28 修回日期:2009-10-12 发布日期:2020-10-26
作者简介:王峥, 女, 博士研究生, 主治医师, 研究方向:肝移植。Tel:13794445996 E-mail:wzsjw@126.com
基金资助:
广东省科技计划项目(2007B031514004)

Effect of ischemia injury on microcirculation of bile ducts relating to liver transplantation and protective effect of recombinant human growth hormone

WANG Zheng, ZHOU Jie, LIN Yi-xiong, CUI Zhong-lin, ZHANG Qi-fan

Department of Hepatobiliary Surgery, Nanfang Hospital, Southern Medical University, Guangzhou 510515, Guangdong, China

Received:2009-08-28 Revised:2009-10-12 Published:2020-10-26

摘要/Abstract

摘要： 目的：探讨大鼠肝移植胆道缺血性损伤对肝内胆道微循环的影响及重组人生长激素的保护作用。方法：建立大鼠自体原位肝移植模型, 随机分为3 组(每组6 只):对照组(CON), 胆道缺血性损伤组(HAL), 重组人生长激素(rhGH) 处理组。于术后7 d 取肝组织, 用兔抗CD34多克隆抗体标记血管, 光镜下观察汇管区血管、胆管并计数。结果：HAL 组肝内胆管上皮细胞排列明显缺失, 汇管区无明显炎症细胞浸润, 汇管区血管及胆管数明显减少, 不伴有血管的胆管数明显增多(P <0.05);rhGH 处理组肝内胆管上皮细胞呈慢性增生性炎症改变, 汇管区血管及胆管数增加, 伴有血管的胆管数明显增多(P <0.05) 。结论：大鼠自体原位肝移植胆道缺血性损伤使肝内胆道微循环受损, 而外源性rhGH 促进肝内胆道微循环的恢复从而对胆道有保护作用。

关键词: 肝移植, 胆道, 微循环, 重组人生长激素

Abstract: AIM: To investigate the changes of microcirculation of bile ducts relating to liver transplantation in rats with ischemia injury and protective effect of recombinant human growth hormone (rhGH) on ischemia injury. METHODS: The rat orthotopic autologous liver transplantation was performed firstly. Wistar rats were randomly divided into three groups each with 6 rats including control group (CON), ischemia injury group (HLA) and rhGH group.Specimen of liver tissue was collected after 7 days post-operation. The blood vessels were tagged with rabbit anti-CD34 polyclonal antibody.The blood vessels and bile ducts of portal area were observed and enumerated. RESULTS: In HAL group, the array of intrahepatic cholangiocytes was significantly absent.There was no obvious soakage of inflammatory cells in portal area. The number of blood vessels and bile ducts in portal area was significantly decreased.The number of bile ducts not accompanying with blood vessels was significantly increased (P <0.05);In rhGH group, the changes of chronic hyperplastic inflammation were oberserved in intrahepatic cholangiocytes.The number of blood vessels and bile ducts in portal area was significantly increased.The number of bile ducts accompanying with blood vessels was significantly increased (P <0.05). CONCLUSION: Ischemia injury of bile ducts relating to rat orthotopic autologous liver transplantation damnifies the microcirculation of intrahepatic bile ducts.However, administration of exogenous rhGH promotes recovery of intrahepatic microcirculation consequently protecting bile ducts from ischemia injury.

Key words: liver transplantation, bile ducts, microcirculation, recombinant human growth hormone

中图分类号:

R619.5

王峥, 周杰, 林艺雄, 崔忠林, 张起帆. 缺血性损伤对肝移植胆道微循环的影响及重组人生长激素的保护作用[J]. 中国临床药理学与治疗学, 2009, 14(11): 1221-1224.

WANG Zheng, ZHOU Jie, LIN Yi-xiong, CUI Zhong-lin, ZHANG Qi-fan. Effect of ischemia injury on microcirculation of bile ducts relating to liver transplantation and protective effect of recombinant human growth hormone[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2009, 14(11): 1221-1224.

参考文献

[1] 黄东胜, 徐骁.肝脏移植的血管并发症及其处理[M] 郑树森, 主编.肝脏移植围手术期处理.北京:人民卫生出版社, 2005:122-125.
[2] Debonero F, Aldeguer X, Shen X, et al.Activation of interleukier 6 STAT3 and liver regeneration following transplantation[J].J Surg Res, 2001, 96(2):289.
[3] 赵宏峰, 周杰.大鼠自体原位肝移植胆道缺血再灌注损伤模型的建立[J].第二军医大学学报, 2006, 27(4):429-430.
[4] 骆助林, 张玉君, 韩本立, 等.门颈静脉转流下大鼠自体原位肝移植模型的建立[J].消化外科, 2005, 4(3):219-220.
[5] Yi C,Wang SR, Zhang SY, et al.Effects of recombinant human growth hormone on acute lung injury in endotoxemic rats[J].Inflamm Res, 2006, 55(11):491-497.
[6] 李榕, 罗春霞, 迟路湘.重组人生长激素对局灶性脑缺血大鼠神经功能恢复的影响[J].中国临床药理学与治疗学, 2007, 12(11):1288-1291.
[7] 蒋忠胜, 温小凤, 柯柳, 等.重组人生长激素治疗失代偿期肝硬化的疗效分析[J].实用肝脏病杂志,2008, 11(6):402-403.
[8] 侍方方, 李苏宜.重组人生长激素联合氟尿嘧啶对人胃癌裸小鼠皮下移植瘤作用的实验研究[J].临床肿瘤学杂志, 2006, 11(6):443-447.
[9] 郭爱平, 李苏宜.重组人生长激素在体外对人胃癌细胞作用的研究[J].东南大学学报:医学版, 2007,47(3):206-209.
[10] Chun EY, Belair L, Jolivet G, et al.Transduction pathways of GH in ovine mammary acini involving regulated and functional growth hormone receptors[J].Growth Factors, 2005, 23(1):55-56.
[11] Waters MJ, Hoang HN, Fairlie DP, et al.New insights into growth hormone action[J].J Mol Endocrinol, 2006, 36(1):1-7.
[12] Perry JK, Emerald BS, Mertani HC, et al.The oncogenic potential of growth hormone[J].Growth Horm IGF Res, 2006, 16(5 6):277-289.

[1]	陈以宁, 肖云, 韩晓雨, 黄璐璐, 马景胜, 熊寒斌, 傅毓, 汪保林, 郭晓晖, 钟林, 敖检根, 何佳珂. 肝移植术后排斥合并高血糖的诊治和用药的实践与思考[J]. 中国临床药理学与治疗学, 2023, 28(5): 550-555.
[2]	吴懿, 方芳, 陈瑛, 樊军卫. 供受体CYP3A5基因型对肝移植术后早期他克莫司谷浓度的影响作用及其临床意义[J]. 中国临床药理学与治疗学, 2021, 26(6): 631-639.
[3]	吴懿, 王让让, 王兆文, 樊军卫. 受体SLCO1B1 rs2291075位点多态性对肝移植术后早期他克莫司剂量校正谷浓度的影响[J]. 中国临床药理学与治疗学, 2020, 25(11): 1268-1275.
[4]	漆勇，张崧，陆才德. 氨甲环酸联合低中心静脉压在肝移植术中的应用研究[J]. 中国临床药理学与治疗学, 2019, 24(3): 307-312.
[5]	颜默磊, 严静, 虞意华, 陈进, 蔡国龙. 多巴胺及去甲肾上腺素对脓毒性休克患者微循环的影响[J]. 中国临床药理学与治疗学, 2013, 18(5): 565-569.
[6]	程敏, 叶小弟, 缪云萍, 陈爱瑛, 郑高利. 珍珠明目滴眼液改善兔眼球结膜微循环的实验研究[J]. 中国临床药理学与治疗学, 2012, 17(8): 856-859.
[7]	韦炳华, 叶毅芳, 罗美娟, 洪晓丹, 李碧虹, 容颖慈, 任斌. 肝移植患者他克莫司个体化给药研究[J]. 中国临床药理学与治疗学, 2012, 17(7): 791-796.
[8]	贺海波, 石孟琼, 卢训丛, 陈涛, 张长城, 王洪武, 袁丁. 珠子参水提物对小鼠耳廓微循环的影响[J]. 中国临床药理学与治疗学, 2010, 15(11): 1216-1222.
[9]	张庆, 陈虹, 张力, 田彦, 李勤, 牛玉坚, 陈新国, 臧运金, 沈中阳. 普乐可复联合护肝片治疗肝移植后轻、中度急性排斥反应的疗效分析[J]. 中国临床药理学与治疗学, 2008, 13(11): 1286-1290.
[10]	李榕, 罗春霞, 迟路湘. 重组人生长激素对局灶性脑缺血大鼠神经功能恢复的影响[J]. 中国临床药理学与治疗学, 2007, 12(11): 1288-1291.
[11]	陶明飞, 杨卫东. 血府逐瘀口服液对小鼠耳廓微循环的影响[J]. 中国临床药理学与治疗学, 2003, 8(1): 89-91.
[12]	吕维名, 钟国连. 山莨菪碱和复方丹参联合治疗糖尿病周围神经病变的临床观察[J]. 中国临床药理学与治疗学, 2002, 7(3): 242-244.
[13]	王春阁, 张弋. 治疗肝移植术后乙肝复发的药物进展[J]. 中国临床药理学与治疗学, 2002, 7(3): 283-285.
[14]	原广平. 复方丹参滴丸对老年糖尿病人甲襞微循环的影响[J]. 中国临床药理学与治疗学, 2002, 7(2): 162-163.
[15]	宋益民, 李学坤, 谭建民, 李增唏, 石山. 阿司匹林、维拉帕米单用和合用对大鼠肠系膜微循环的影响¹[J]. 中国临床药理学与治疗学, 2001, 6(1): 31-34.