肉苁蓉总苷对小鼠酒精性肝损伤的保护作用

中国临床药理学与治疗学 ›› 2009, Vol. 14 ›› Issue (11): 1225-1228.

肉苁蓉总苷对小鼠酒精性肝损伤的保护作用

罗慧英, 刘渊, 席国柱, 杜建德

甘肃中医学院药理教研室, 兰州 730000, 甘肃

收稿日期:2009-10-10 修回日期:2009-11-12 发布日期:2020-10-26
作者简介:罗慧英, 女, 博士, 副教授, 硕士生导师, 研究方向:保肝与抗肿瘤药物研究。Tel:0931-8765395 　E-mai l:louri a@vip.sina.com
基金资助:
甘肃省教育厅科研项目(0906B-01)

Protective effect of Cistanchis glycosides on ethanol-induced liver damage in mice

LUO Hui-ying, LIU Yuan, XI Guo-zhu, DU Jian-de

Department of Pharmacology, Gansu College of Traditional Chinese Medicine, Lanzhou 730000, Gansu, China

Received:2009-10-10 Revised:2009-11-12 Published:2020-10-26

摘要/Abstract

摘要： 目的：研究肉苁蓉总苷对小鼠酒精性肝损伤的保护作用。方法：昆明种小鼠40 只, 随机分为空白组、模型组、肉苁蓉总苷小剂量组(50 mg/kg)和大剂量组(100 mg/kg)。连续灌胃给药4 周, 肉苁蓉总苷每日2 次, 酒精每日1 次, 到达时限后小鼠称重, 眶静脉(动脉)取血, 取血清检测肝功指标;摘取肝脏称重, 计算肝脏指数;剪取适量肝脏制成匀浆, 试剂盒检测相关生化指标;剩余肝脏10 %甲醛固定后进行病理学检测。结果：小鼠酒精肝损伤后肝脏指数、肝功指标明显升高(P <0.05);肝脏组织中TG 、TC 、丙二醛(MDA)、脂质过氧化物(LPO)含量明显高于空白组(P <0.05);超氧化物歧化酶(SOD)、谷胱甘肽还原酶(GSH-Px)、过氧化氢酶(CAT)活性明显降低(P <0.05);病理学检测发现肝脏组织出现炎性浸润和脂肪空泡。肉苁蓉总苷可明显改善这些现象, 表现为降低肝脏指数和肝功指标(P <0.05), 减少肝脏组织中TG 、TC 、MDA 、LPO 含量(P <0.05), 增强SOD 、GSH-Px 和CAT 活性(P <0.05), 减少肝脏组织炎性浸润和脂肪空泡的产生。其中大剂量肉苁蓉总苷的作用更加明显。结论：肉苁蓉总苷可能通过减少自由基的产生, 增强对自由基及其代谢产物的清除能力, 从而抑制脂质过氧化, 起到对酒精性肝损伤的保护作用。

关键词: 肉苁蓉总苷, 酒精性肝损伤, 肝功能, 脂质过氧化

Abstract: AIM: To research the protection of Cistanchis glycosides on ethanol-induced liver damage in mice. METHODS: 40 mice were divided into 4 groups in random, and administered by gavage for 4 weeks.Cistanchis glycosides were twice a day, ethanol were once a day.When time limit arrived, mice were weighted, the serum was afforded to detect the liver function index, liver homogenate were prepared for biochemical detections, liver remained were fixed for pathological investigation. RESULTS: When mice were damaged by ethanol, liver index, liver function index, contents of TG, TC, MDA and LPO were increased distinctly(P <0.05), The activities of SOD, GSH-Px and CAT were decreased at the same time(P <0.05).Imflammatory infiltration and flat vacuoles were observed.Cistanchis glycosides ameliorated these changes evidently(P <0.05);the effects were dosedependence. CONCLUSION: Protective effect of Cistanchis glycosides on ethanol-induced liver damage in mice is associated with antioxidation, enhancement of clearance for free radicals and metabolites, and inhibition of lipid overoxidation reactions.

Key words: Cistanchis glycosides, ethanol-induced liver damage, liver function, lipid overoxidation reactions

中图分类号:

R965.2

罗慧英, 刘渊, 席国柱, 杜建德. 肉苁蓉总苷对小鼠酒精性肝损伤的保护作用[J]. 中国临床药理学与治疗学, 2009, 14(11): 1225-1228.

LUO Hui-ying, LIU Yuan, XI Guo-zhu, DU Jian-de. Protective effect of Cistanchis glycosides on ethanol-induced liver damage in mice[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2009, 14(11): 1225-1228.

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