特异性免疫治疗对哮喘大鼠转化生长因子-β1/ Smad 信号通路的影响

中国临床药理学与治疗学 ›› 2009, Vol. 14 ›› Issue (11): 1229-1233.

特异性免疫治疗对哮喘大鼠转化生长因子-β₁/ Smad 信号通路的影响

李绍波¹, 金小红¹, 王昕昕¹, 赖小英¹, 童夏生²

¹台州医院儿内科, 临海317000, 浙江;
²台州中西医结合医院儿内科, 温岭 317523, 浙江

收稿日期:2009-10-07 修回日期:2009-11-12 发布日期:2020-10-26
作者简介:李绍波, 男, 硕士, 主治医师, 研究方向:儿科呼吸。Tel:13958578102 E-mail:lishaobo71@yahoo.com.cn
基金资助:
浙江省医药卫生科技计划(2008B192 ); 台州市科技局课题(061KY06)

Effect of specific immunotherapy on TGF-β₁/ Smads in asthmatic rats

LI Shao-bo, JIN Xiao-hong, WANG Xin-xin, LAI Xiao-ying, TONG Xia-sheng

Department of Internal Medicine, Taizhou Hospital, Linhai 317000, Zhejiang, China; Department of InternalMedicine, Combination of TCM with Western Medicine of Taizhou, Wenling 317523, Zhejiang, China

Received:2009-10-07 Revised:2009-11-12 Published:2020-10-26

摘要/Abstract

摘要： 目的：探讨特异性免疫治疗(specific immunotherapy,SIT) 对哮喘大鼠转化生长因子-β₁(TGF-β₁) 及信号转导分子Smads 表达的影响。方法：40只健康雄性清洁级Wistar 大鼠随机均分成正常对照组、哮喘组、SIT 对照组和SIT 治疗组等4 组, 每小组10 只。通过卵蛋白(OVA) 雾化吸入的方法对致敏大鼠进行SIT, 用双抗体夹心ELISA 法测定血清和BALF 中TGF-β₁浓度, 免疫组化方法检测肺组织TGF-β¹以及磷酸化Smad2/3 (P-Smad2/3) 、Smad7 蛋白表达水平。结果：哮喘组和SIT 治疗组的血清、BALF 中TGF-β₁ 浓度均分别高于正常对照组, 但SIT 治疗组与哮喘组相比, 两者的TGF-β₁ 浓度则均有所下降;哮喘组肺组织TGF-β₁和P-Smad2/3 蛋白表达较正常对照组和SIT 治疗组增高, 而Smad7 蛋白表达下降。结论：SIT 通过抑制哮喘大鼠体内TGF-β₁和P-Smad2/3 的过度表达及上调Smad7, 从而阻断TGF-β₁的胞内信号转导, 可在一定程度上减轻哮喘大鼠气道炎症和抑制气道重塑的发生发展。

关键词: 哮喘, 特异性免疫治疗, 转化生长因子-β₁;, Smads

Abstract: AIM: To approach the effect of specific immunotherapy on transforming growth factor (TGF-β₁) Smads in asthmatic rats. METHODS: Forty Wistar rats were randomly divided into 4 groups:control group, asthma group, control of specific immunotherapy group and specific immunotherapy group, with 10 rats in each group.The model of asthma was established by the ovalbumin (OVA) challenge methods and the specific immunotherapy group received specific immunotherapy by repeated inhaling different doses of OVA. The concentrations of TGF-β₁ in BALF and serum were detected by ELISA.The expressions of TGF-β₁, phosphorylated Smad2/3(P-Smad2/3) and Smad7 proteins in lung tissue were detected by immunocytochemical method. RESULTS: In BALF or serum, the concentration of TGF-β₁ in control group was lower than that in asthma group or specific immunotherapy group respectively.The concentrations of TGF-β₁ in BALF or serum in specific immunotherapy group was lower than that in asthma group.The expressions of TGF-β₁ and PSmad2/3 in lung in asthma group were higher, but the expressions of Smad7 was lower than that in control group or in specific immunotherapy group respectively. CONCLUSION: Specific immunotherapy could decrease the expressions of TGF-β₁ and P-Smad2/3, while increase the expression of Smad7 in asthmatic rat, and relieve the airway inflammation in some extent and inhibit the occurrence and development of airway remodeling.

Key words: asthma, specific immunotherapy, TGF-β₁;, Smads

中图分类号:

R965.2

李绍波, 金小红, 王昕昕, 赖小英, 童夏生. 特异性免疫治疗对哮喘大鼠转化生长因子-β₁/ Smad 信号通路的影响[J]. 中国临床药理学与治疗学, 2009, 14(11): 1229-1233.

LI Shao-bo, JIN Xiao-hong, WANG Xin-xin, LAI Xiao-ying, TONG Xia-sheng. Effect of specific immunotherapy on TGF-β₁/ Smads in asthmatic rats[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2009, 14(11): 1229-1233.

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特异性免疫治疗对哮喘大鼠转化生长因子-β₁/ Smad 信号通路的影响

Effect of specific immunotherapy on TGF-β₁/ Smads in asthmatic rats

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