注射卡介苗素对接种乙型肝炎疫苗无、弱应答婴儿血IL-10 、IL-12 的影响

中国临床药理学与治疗学 ›› 2009, Vol. 14 ›› Issue (11): 1296-1299.

注射卡介苗素对接种乙型肝炎疫苗无、弱应答婴儿血IL-10 、IL-12 的影响

狄军波, 陈益平, 徐志伟

温州医学院附属育英儿童医院感染科, 温州325027, 浙江

收稿日期:2009-08-27 修回日期:2009-11-05 发布日期:2020-10-26
作者简介:狄军波, 男, 硕士, 主治医生, 研究方向:儿童感染性疾病。Tel:13676725353 E-mail:djb1976@sohu.com
基金资助:
温州医学院课题基金项目(2005-35)

Study on IL-12, IL-10 expression of infants with none or low response to recombinant hepatitis B vaccine stimulated by bacillus Calmette-Guerin

DI Jun-bo, CHEN Yi-ping, XU Zhi-wei

Department of Infection, YuYing Children's Hospital Affiliated to Wenzhou Medical College, Wenzhou 325027, Zhejiang, China

Received:2009-08-27 Revised:2009-11-05 Published:2020-10-26

摘要/Abstract

摘要： 目的：探讨注射卡介苗素对接种乙型肝炎疫苗后无、弱应答婴儿血清IL-12 和IL-10 表达的作用。方法：放免法定量检测按0 、1 、6 方案(每次10 μg) 标准全程接种重组乙型肝炎疫苗后婴儿表面抗体(Anti-HBs) 的几何平均滴度(geometric meantiter, GMT), 筛选无或弱应答者(GMT <100 UL) 60例, 随机分为两组各30 例进行疫苗复种, 其中一组同时肌内注射卡介苗素。用ELISA 法检测复种前后血清中IL-12 、IL-10 的浓度。结果：注射卡介苗素能诱导无、弱应答婴儿产生较高水平的IL-12 、IL-10, 并呈疗程依赖性, 卡介苗注射组的抗体应答水平较对照组高, 两者差异有统计学意义。结论：卡介苗素能有效诱导无、弱应答婴儿机体产生细胞因子IL-12 、IL-10, 为卡介苗素有望作为一种较好的乙肝疫苗佐剂提供了实验基础。

关键词: 卡介苗素, 重组乙型肝炎疫苗, IL-10, IL-12

Abstract: AIM: To investigate the efficacy of Bacillus Calmette-Guerin (BCG) on IL-12, IL-10 expressions of infants with none or low response to recombinant hepatitis B vaccine. METHODS: The geometric mean titer (GMT) of anti-HBs was tested by radioimmunity assay after hepatitis B vaccine stimulation, selected 60 cases with non-or low-response(GMT <100 UL), who were divided into two groups, one group was injected by BCG, the concentrations of IL-10, IL-12 were detected by ELISA. RESULTS: BCG could significantly stimulate infants with none or low response to recombinant hepatitis B vaccine to product IL-12 and IL-10 associated cytokines, the enhancement was course-dependent.Compared with the control group, the GMT of anti-HBs was significantly higher than that in the Bacillus Calmette-Guerin injection group. CONCLUSION: BCG might be an effective adjuvant to induce cellular immune response via the enhancement of IL-12 and IL-10 of infants with none or low response to recombinant hepatitis B vaccine.

Key words: Bacillus Calmette-Guerin, recombinant hepatitis B vaccine, IL-12, IL-10

中图分类号:

R969

狄军波, 陈益平, 徐志伟. 注射卡介苗素对接种乙型肝炎疫苗无、弱应答婴儿血IL-10 、IL-12 的影响[J]. 中国临床药理学与治疗学, 2009, 14(11): 1296-1299.

DI Jun-bo, CHEN Yi-ping, XU Zhi-wei. Study on IL-12, IL-10 expression of infants with none or low response to recombinant hepatitis B vaccine stimulated by bacillus Calmette-Guerin[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2009, 14(11): 1296-1299.

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