苏郁胶囊对双侧嗅球损伤抑郁模型大鼠行为学及下丘脑-垂体-肾上腺轴的影响

中国临床药理学与治疗学 ›› 2009, Vol. 14 ›› Issue (2): 163-166.

苏郁胶囊对双侧嗅球损伤抑郁模型大鼠行为学及下丘脑-垂体-肾上腺轴的影响

黄真¹, 毛庆秋², 潘爱娟¹, 冯春荣¹, 李兆翌¹, 钟晓明¹

¹浙江中医药大学药学院, 杭州310053, 浙江;
²香港中文大学中医学院, 新界沙田999077, 香港

收稿日期:2008-10-08 修回日期:2009-01-19 出版日期:2009-02-26 发布日期:2020-10-30
作者简介:黄真, 女, 教授, 博导, 研究方向:治疗神经精神系统疾病中药新药研究。Tel:0571-86613576 　E-mail:zhen626@yahoo.com.cn
基金资助:
卫生部科学研究基金-浙江省医药卫生重大科技计划(WKJ2007-2-020);浙江省卫生高层次创新人才培养工程项目;浙江省自然科学基金项目(X206952);浙江省中医药科技计划重点项目 (2006Z001)

Effects of Suyu capsule on behaviors and hypothalamus-pituitary-adrenal axis in depression model rats induced by bilateral olfactory bulb impairment

HUANG Zhen¹, MAO Qing-qiu², PAN Ai-juan¹, FENG Chun-rong¹, LI Zhao-yi¹, ZHONG Xiao-ming¹

¹Zhejiang Chinese Medicine University, College of pharmacy, Hangzhou 310053, Zhejiang, China;
²The Chinese University of Hong Kong School of Chinese Medicine, NT Shatin 999077, Hong Kong, China

Received:2008-10-08 Revised:2009-01-19 Online:2009-02-26 Published:2020-10-30

摘要/Abstract

摘要： 目的:探讨苏郁胶囊对双侧嗅球损伤抑郁模型大鼠行为学及下丘脑-垂体-肾上腺(HPA)轴的影响。方法:48 只雄性大鼠随机分为假手术组、模型组、苏郁胶囊高、中、低剂量组(22.8 、11.4 、5.7 g/kg)及氯米帕明组(0.02 g/kg)。应用双侧嗅球损伤复制大鼠抑郁模型, 敞箱法及跳台法测定大鼠的行为学指标;放射免疫方法测定大鼠血浆促肾上腺皮质素释放激素(CRH)、促肾上腺皮质激素(ACTH)及皮质醇(CORT)的水平。结果:苏郁胶囊可不同程度降低双侧嗅球损伤大鼠敞箱实验的水平运动次数与垂直运动次数;增加双侧嗅球损伤大鼠跳台实验的潜伏期及减少错误次数;同时降低双侧嗅球损伤大鼠血浆CRH、ACTH 和CORT 含量。结论:苏郁胶囊能逆转双侧嗅球损伤抑郁模型大鼠的行为异常, 其可能与苏郁胶囊有效地拮抗HPA 轴功能亢进相关。

关键词: 苏郁胶囊, 抑郁症, 双侧嗅球损伤, 行为学, 下丘脑-垂体-肾上腺轴

Abstract: AIM: To study the effects of Suyu capsule on behaviors and hypothalamus-pituitary-adrenal axis in depression model rats induced by bilateral olfactory bulb impairment.METHODS: 48 male SD rats were randomly divided into 6 groups including the sham group, the model group, and three Suyu capsule groups(The doses were 22.8, 11.4, 5.7 g/kg, respectively) and Clomipramine group(0.02 g/kg).The depression model was established by bilateral olfactory bulb impairment.The behaviors of rats were observed using open-field and step-down test.The levels of CRH, ACTH and CORT in plasma of rats were detected by radioimmunoassay (RIA).RESULTS: Suyu capsule could decrease the numbers of crossing and rearing of depression model rats using the Open-Field test.Suyu capsule increased the latent time and diminish the mistakes for the depression model rats in the step-down test, Suyu capsule also decreased the levels of CRH, ACTH and CORT in plasma of depression model rats.CONCLUSION: Suyu capsule could reverse the behavioral changes of depression model rats induced by bilateral olfactory bulb impairment, which may be related to Suyu capsule effectively antagonize hypothalamus-pituitary-adrenal axis hyperfunction.

Key words: Suyu capsule, depressive disorder, bilateral olfactory bulb impairment, behavior, hypothalamus-pituitary-adrenal axis

中图分类号:

R285.5

黄真, 毛庆秋, 潘爱娟, 冯春荣, 李兆翌, 钟晓明. 苏郁胶囊对双侧嗅球损伤抑郁模型大鼠行为学及下丘脑-垂体-肾上腺轴的影响[J]. 中国临床药理学与治疗学, 2009, 14(2): 163-166.

HUANG Zhen, MAO Qing-qiu, PAN Ai-juan, FENG Chun-rong, LI Zhao-yi, ZHONG Xiao-ming. Effects of Suyu capsule on behaviors and hypothalamus-pituitary-adrenal axis in depression model rats induced by bilateral olfactory bulb impairment[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2009, 14(2): 163-166.

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