甘草酸二铵磷脂复合物对小鼠慢性肝损伤的保护作用

中国临床药理学与治疗学 ›› 2009, Vol. 14 ›› Issue (6): 642-645.

甘草酸二铵磷脂复合物对小鼠慢性肝损伤的保护作用

茹仁萍¹, 陈晓瑾¹, 尤琳雅¹, 唐红英¹, 蔡汉萍¹, 匡荣²

¹杭州市第六人民医院药剂科, 杭州 310004, 浙江;
²浙江省食品药品检验所, 杭州 310004, 浙江

收稿日期:2009-05-21 修回日期:2009-06-25 出版日期:2009-06-26 发布日期:2020-10-27
作者简介:茹仁萍,女,主任药师,研究方向:医院药学。Tel:0571-85463950 E-mail:rrprrp@126.com
基金资助:
杭州市科技局项目(20051333838)

Protective effects of diammonium glycyrrhizinate phospholipid complex on chronic liver injury

RU Ren-ping¹, CHEN Xiao-jin¹, YOU Lin-ya¹, TANG Hong-ying¹, CAI Han-ping¹, KUANG Rong²

¹Pharmacy of the Sixth People's Hospital of Hangzhou, Hangzhou 310004, Zhejiang, China;
²Zhejiang Institute for Food and Drug Control, Hangzhou 310004, Zhejiang, China

Received:2009-05-21 Revised:2009-06-25 Online:2009-06-26 Published:2020-10-27

摘要/Abstract

摘要： 目的: 探讨甘草酸二铵磷脂复合物注射剂(DG-PC)对C57BL/6J 小鼠慢性免疫性肝脏损害的保护作用。方法: 复制C57BL/6J 小鼠慢性免疫性肝脏损害模型, 测定血清ALT、AST、蛋白和透明质酸(HA), 肝匀浆中超氧化物岐化酶(SOD)、谷胱甘肽(GSH)、I 型胶原(C.I), 并对肝脏组织切片进行电镜观察。结果: DG-PC 可以明显改善肝损小鼠AST 水平、提高白蛋白(ALB)含量、升高白球比(A G)、降低HA 含量(P<0.01), 提高肝损小鼠的SOD 和GSH 水平(P<0.01), 治疗效果优于甘草酸二铵(DG)。小鼠肝组织透射电镜观察显示DG-PC 可以保护肝细胞和细胞器, 减轻纤维化程度。结论: DG-PC 对于C57 BL/6J 小鼠慢性免疫性肝损伤均具有一定的保护作用, 可能与抗氧化等活性有关。

关键词: 甘草酸二铵磷脂复合物, 慢性免疫性肝损伤, 超微结构

Abstract: AIM: To approach the protective effects of diammonium glycyrrhizinate phospholipid complex injection (DG-PC)on chronic and immunological liver injury. METHODS: The chronic and immunological hepatic liver injury was established in C57BL/6J mice, the levels of ALT, AST, TP,ALB and HA in serum were determined, the contents of SOD,GSH and collagen 1 (C.I)in liver homogenate were detected, and the liver tissue sections were observed by electron microscope. RESULTS: Compared with DG, DG-PC could significantly improve the AST level, increase the content of ALB, increase the A/G, decrease the content of HA(P<0.01), improve the activities of SOD and GSH(P<0.01).The therapeutic efficacy of DG-PC was better than that of DG.As to microstructure, DGPC could protect the hepatic cell and organelle, and relieve the fibrosis level. CONCLUSION: DG-PC has protective effects on chronic and immunological liver damage to C57 BL/6J mice, which maybe relate to the antioxidant effects.

Key words: diammonium glycyrrhizinate phospholipid complex, chronic and immunological liver injury, ultrastructure

中图分类号:

R965.2

茹仁萍, 陈晓瑾, 尤琳雅, 唐红英, 蔡汉萍, 匡荣. 甘草酸二铵磷脂复合物对小鼠慢性肝损伤的保护作用[J]. 中国临床药理学与治疗学, 2009, 14(6): 642-645.

RU Ren-ping, CHEN Xiao-jin, YOU Lin-ya, TANG Hong-ying, CAI Han-ping, KUANG Rong. Protective effects of diammonium glycyrrhizinate phospholipid complex on chronic liver injury[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2009, 14(6): 642-645.

参考文献

[1] Hu YJ, Liu Y, Wang JB, et al.Study of the interaction between monoammonium glycyrrhizinate and bovine serum albumin[J].J Pharm Biomed Anal, 2004, 36(4):915-919.
[2] 张祥沛.磷脂酰胆碱对小鼠过氧化损伤的保护作用[J].中国公共卫生, 2002, 18(1):44.
[3] 江绍基.临床肝胆系病学[M].上海:上海科学技术出版社, 1994:230.
[4] 高寿征.病毒性肝炎防治研究[M].北京:北京出版社, 1993:77.
[5] 马正业, 黄启福, 贾旭, 等.C₅₇BL/6 小鼠免疫性肝损伤模型复制[J].J China-Japan Friendship Hospital,1996, 10(4):291-293.
[6] Louse AW, Manns M, Dienes HP, et al.Experimental autoimmune hepatitis:disease induction, time course and T-cell reactivity[J].Hepatology, 1990, 11(1):24-30.
[7] 罗洋, 都飞, 王宇明, 等.自身免疫性肝炎小鼠动物实验模型的建立[J].Chin J Hepatol, 2001, 9(6):358-360.
[8] 茹仁萍, 吴锡铭, 朱幸芳.小鼠慢性免疫性肝损伤模型建立与筛选[J].医学研究通讯, 2005, 34(12):31-33.
[9] Tsuji T, Shinohara T.Pathological study of chronic Dgalactosamine induced hepatitis in mice by administration of adjuvants-an animal model of the chronic active hepatitis [J].Gastroenterol Jpn, 1981, 16(1):9-20.
[10] 王迪生, 高继君, 张承光, 等.赤芍甘草冲剂对D-半乳糖诱导肝损伤的保护作用[J].中国临床药理学与治疗学, 2006, 11(9):1026-1029.
[11] Rice-Evans C.Alternation in erythrocyte membrane fluidity by pheny lhdrazine induced peroxidation of lipids[J]. Biophys Res Commun, 1981, 100:1537.
[12] 胡小玲, 汪晖, 吴基良.阿魏酸钠的抗肝损伤作用及其机制研究[J].中国临床药理学与治疗学, 2004, 9(1):74-76.
[13] Ma X, Zhao J, Lieber CS.Polyenylphosphatidylcholine attenuates non-alcoholic fibrosis and accelerates its regression[J].J Hepatol, 1996, 24(5):604-613.
[14] Navder KP, Baraona E, Liebe CS. Polyenylphosphatidylcholine attenuates alcohol-induced fatty liver and hyperlipemia in rats[J].J Nutr, 1997, 127(9):1800-1806.