辛伐他汀减轻舒张功能不全大鼠心肌线粒体损伤

中国临床药理学与治疗学 ›› 2009, Vol. 14 ›› Issue (7): 780-784.

辛伐他汀减轻舒张功能不全大鼠心肌线粒体损伤

唐惠芳^1,2, 吴书林¹, 邓春玉¹, 薛玉梅¹

¹广东省人民医院心血管内科, 广州510100, 广东;
²南华大学附属第一医院心血管内科, 衡阳421001, 湖南

收稿日期:2009-04-27 修回日期:2009-07-21 出版日期:2009-07-26 发布日期:2020-10-30
通讯作者: 吴书林, 男, 博士, 教授, 博士研究生导师, 主要从事心衰和心血管疾病电生理研究。Tel:020-83827812-10290 　E-mail:sunsunny999@163.com
作者简介:唐惠芳, 女, 博士, 副主任医师, 硕士生导师, 主要从事心衰和心血管疾病电生理研究。Tel:0734-8279353 　E-mail:tanghuifang999@163.com

Protective effect of simvastatin on cardiac mitochondrion of rats with diastolic heart failure

TANG Hui-fang^1,2, WU Shu-lin¹, DENG Chun-yu¹, XUE Yu-mei¹

¹Department of Cardiology, Guangdong Provincial People's Hospital, Guangzhou 510100, Guangdong, China;
²The First Affiliated Hospital of University of South China, Hengyang 421001, Hunan, China

Received:2009-04-27 Revised:2009-07-21 Online:2009-07-26 Published:2020-10-30

摘要/Abstract

摘要： 目的:探讨辛伐他汀对舒张功能不全心衰(DHF) 大鼠心脏功能的作用及机制。方法:50 只雄性SD 大鼠, 随机分为对照组、DHF 组、辛伐他汀1 mg 组(S1 组)、2 mg 组(S2 组) 和4 mg 组(S4组)。采用腹主动脉缩窄建立DHF 模型, S1、S2、S4 组大鼠术后灌胃分别给予辛伐他汀1、2、4 mg°kg^-1°d^-1, 对照组和DHF 组大鼠给予同等量生理盐水。4 周末心脏B 超检测心功能, 颈动脉插管记录血流动力学, 分光光度计检测心肌线粒体丙二醛(MDA)、超氧化物歧化酶(SOD) 和谷胱甘肽过氧化物酶(GSH-Px) 含量, 电镜检测心肌线粒体超微结构。结果:DHF 组大鼠左室后壁(LVPW)、室间隔(IVS)、左室心脏指数(LVM)、E A比值增高, 左室收缩压(LVSP)、左室舒张末期压(LVEDP) 升高, 左室松弛时间常数(Tau) 延长, 平均左室内压最大下降速率(LV-dp d t_max) 下降, 心肌SOD 和GSH-Px 下降、MDA 增加, 电镜示心肌细胞肌丝排列不整齐、线粒体肿胀及空泡化等线粒体损伤。辛伐他汀改善DHF 大鼠心功能和血流动力学指标, 同时减轻线粒体结构和功能损伤, 随着辛伐他汀剂量的增加, 以上指标改善程度越明显。结论:辛伐他汀减轻DHF 大鼠心肌细胞和线粒体损伤是辛伐他汀保护DHF 大鼠心功能的重要机制。

关键词: 辛伐他汀, 舒张功能不全, 心肌细胞, 线粒体

Abstract: AIM: To study the effect of simvastatin on cardiac function and mitochondrion of rats with diastolic heart failure (DHF) and its mechanism.METHODS: 50 SD rats were randomly divided into 5 groups: control group, DHF group, simvastatin 1mg group (S1 group), simvastatin 2mg group(S2 group) and simvastatin 4 mg group (S4 group).DHF model was produced by abdominal aortic coarctation in rats of the operating group.The rats in simvastatin groups were given with simvastatin (1, 2, 4 mg°kg^-1 °d^-1) by intragastric administration for 4 weeks, the others were treated with equal isotonic Na chloride.After 4 weeks, the cardiac function and hemorheology were assessed by echocardiography and catheterization.The level of malonaldehyole (MDA), superoxidedismutase (SOD) and glutathioneperoxidase(GSH-Px) were detected by spectrophotometer.Ultra structure changes in myocardium were observed under electron microscope.RESULTS: In DHF group, the left ventricular posterior wall (LVPW), interventricular septum (IVS), left ventricular cardiac index (LVM), E A ratio, left ventricular systolic pressure (LVSP), left ventricular end-diastolic pressure (LVEDP) and MDA were increased, the left ventricular relaxation time (Tau) was prolonged, the LV-dp dt_max, SOD and GSH-Px were decreased.The myocardium damage including abnormality of myofilament arrangement, mitochondrial swelling and vacuolization were showed.Compared with DHF group, the cardiac function and hemorheology were improved in the treatment groups.The myocardium damage was ameliorated in the simvastatin group too.The protective effect of simvastatin on heart was increased according to the dose of simvastatin.CONCLUSION: Simvastatin improves cardiac diastolic function in rats with DHF by ameliorating the damage of myocardial cell and mitochondrion.

Key words: simvastatin, diastolic heart failure, myocardial cell, mitochondrion

中图分类号:

R332

唐惠芳, 吴书林, 邓春玉, 薛玉梅. 辛伐他汀减轻舒张功能不全大鼠心肌线粒体损伤[J]. 中国临床药理学与治疗学, 2009, 14(7): 780-784.

TANG Hui-fang, WU Shu-lin, DENG Chun-yu, XUE Yu-mei. Protective effect of simvastatin on cardiac mitochondrion of rats with diastolic heart failure[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2009, 14(7): 780-784.

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