转染p21基因对人动脉平滑肌细胞的影响

中国临床药理学与治疗学 ›› 2009, Vol. 14 ›› Issue (9): 1013-1017.

转染p21基因对人动脉平滑肌细胞的影响

林丙来, 姜兰兰, 方五旺

芜湖市第二人民医院心内二科, 芜湖241000, 安徽

收稿日期:2009-07-12 修回日期:2009-08-28 发布日期:2020-11-03
通讯作者: 方五旺, 男, 博士, 主任医师, 研究方向:心血管内科疾病的诊治。Tel:13705530450 E-mail: fangwuwang@sina.com
作者简介:林丙来, 男, 本科, 主治医师, 研究方向:心血管内科疾病的诊治。Tel:13085536072 E-mail: linbl2002@sina.com
基金资助:
芜湖市2007 年科技计划重点项目(芜科计[2007] 126 号)

Effects of p21 gene transfection in human arterial smooth muscle cells

LIN Bing-lai, JIANG Lan-lan, FANG Wu-wang

Second Department of Cardiology, the Second People's Hospital of Wuhu, Wuhu 241000, Anhui, China

Received:2009-07-12 Revised:2009-08-28 Published:2020-11-03

摘要/Abstract

摘要： 目的: 探讨转染p21 基因对人动脉平滑肌细胞(HASMC) 的影响, 以探讨转染p21 基因实现抗冠状动脉支架内再狭窄的作用, 为冠状动脉支架内再狭窄的治疗提供新思路。方法: 以Lipofectamine2000 脂质体介导p21 基因转染HASMC株;免疫组化法检测转染p21 基因后, 其编码蛋白在HASMCs 的表达情况;描绘p21 基因转染后HASMCs 的增殖曲线;WST-1 法测定OD 值, 计算细胞生长抑制率及流式细胞仪检测p21 基因转染对HASMCs 凋亡的影响。结果: 免疫组化法显示p21 基因成功转入HASMCs 后, 其编码的蛋白能在细胞核内进行高表达;转染p21 基因的HASMCs在体外生长曲线较对照组明显降低;WST-1 法显示转染p21 基因的HASMCs 细胞活力与对照组相比明显降低, 差异有统计学意义(P <0.05) ;流式细胞仪检测发现转染p21 基因的HASMCs 细胞凋亡率达40.26 %+0.013 %, 且显著高于对照组, 差异有统计学意义(P <0.05) 。结论: 成功将p21基因转入HASMCs, 其编码蛋白可能参与了抑制细胞生长和诱导细胞凋亡作用, 提示p21 基因转染HASMCs 技术有可能为人冠状动脉支架内再狭窄的治疗提供一种新的防治策略与手段。

关键词: p21 基因, 转染, 人动脉平滑肌细胞, 凋亡

Abstract: AIM: To find a new way for the treatment of restenosis after coronary artery inserted stent, the effect of p21 gene transfection in human arterial smooth muscle cells (HASMCs) was investigated and its antagonistic effect of restenosis after coronary artery inserted stent was studied. METHODS: P21 gene was transfected into HASMC via Lipofectamine 2000 liposomes and the expression of its encoded protein in HASMCs was detected by immunohistochemistry. The effects of p21 gene transfection on the growth, proliferation and apoptosis of HASMCs were described by cell growth curves, the WST-1 assay and flow cytometry (FCM) analysis respectively. RESULTS: Immunohistochemical result indicated that the protein encoded by p21 gene was highly expressed in the cytoblast of HASMCs. The expression of p21 gene significantly inhibited the growth and proliferation of HASMC and remarkably promoted its apoptosis. Compared with the control group, the difference was statistically significant. CONCLUSION: These results demonstrate that the encoded protein of the p21 gene transfected into HASMCs may be involved in inhibiting cell growth and inducing apoptosis, suggesting that the technology of p21 gene transfected into HASMCs can be a new strategy to prevent and treat restenosis after coronary artery inserted stent.

Key words: p21 gene, transfection, HASMC, apoptosis

中图分类号:

R965.2

林丙来, 姜兰兰, 方五旺. 转染p21基因对人动脉平滑肌细胞的影响[J]. 中国临床药理学与治疗学, 2009, 14(9): 1013-1017.

LIN Bing-lai, JIANG Lan-lan, FANG Wu-wang. Effects of p21 gene transfection in human arterial smooth muscle cells[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2009, 14(9): 1013-1017.

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