缬沙坦干预对缺氧性肺动脉高压大鼠大电导钙激活钾通道的影响

中国临床药理学与治疗学 ›› 2009, Vol. 14 ›› Issue (9): 1032-1035.

缬沙坦干预对缺氧性肺动脉高压大鼠大电导钙激活钾通道的影响

王新¹, 杨玉雯¹, 王安才¹, 孔祥权²

¹皖南医学院附属弋矶山医院老年医学科,
²急诊内科, 芜湖241001, 安徽

收稿日期:2009-08-14 修回日期:2009-09-08 发布日期:2020-11-03
通讯作者: 杨玉雯, 女, 主任医师, 硕士生导师, 研究方向:心脏电生理。王安才,男, 主任医师, 硕士生导师, 研究方向:高血压及冠心病诊治。
作者简介:王新, 女, 本科, 副主任医师, 研究方向:为高血压及冠心病诊疗。Tel:0553-5739308 E-mail: xinwangandyou@yahoo.com.cn

Effects of valsartan on BKCa of hypoxic pulmonary hypertensive rats

WANG Xin¹, YANG Yu-wen¹, WANG An-cai¹, KONG Xiang-quan²

¹Department of Geraeology Yijishan Hospital of Wannan Medical College;
²Department of Emergenly Yijishan Hospital of Wannan Medical College, Wuhu 241001, Anhui, China

Received:2009-08-14 Revised:2009-09-08 Published:2020-11-03

摘要/Abstract

摘要： 目的: 观察缬沙坦对缺氧性肺动脉高压(PAH)大鼠肺动脉平滑肌细胞大电导钙激活钾通道(large-conductance calcium-activated potassiumchannel, BKCa, MaxiK)的影响。方法: 选取二级健康7 周龄雄性SD 大鼠30 只, 随机分为A 组:对照组,B 组:PAH 模型组,C 组:缬沙坦干预组。测定3 组大鼠肺动脉平均压(PAMP), 利用膜片钳全细胞模式记录肠系膜动脉平滑肌细胞膜电容、钾电流以及用四乙胺阻断BKCa 后的电流, 以计算BKCa 电流值及其电流密度值, 免疫组织化学染色观察BKCa 蛋白的表达。结果: B 组PAMP 明显高于A 组(P <0.01), 而膜电容、BKCa 电流和电流密度及BKCa 蛋白表达平均积分吸光度(iOD)值均明显低于A 组(P <0.05)。与B 组相比较, 给予缬沙坦干预后, C 组的PAMP 显著下降(P <0.01), BKCa 电流和电流密度均有不同程度增加(P <0.05), 而BKCa 蛋白表达iOD 值则无明显变化。结论: 缬沙坦可有效降低缺氧性肺动脉高压大鼠的肺动脉压力, 其机制可能是通过增加肺动脉平滑肌细胞BKCa 的活性实现的。

关键词: 缬沙坦, 大电导钙激活钾通道, 膜片钳技术, 肺动脉高压

Abstract: AIM: To observe the effects of valsartan on the large-conductance calcium-activated potassium channel (BKCa)of smooth muscle cells isolated from hypoxia-pulmonary artery hypertension (PAH) rats. METHODS: Thirty second-grade healthy SD male rats aged 7 weeks were selected, and divided randomly into 3 groups: Group A was control group, Group B was PAH group and Group C was valsartan intervention group with 10 rats in each group. The mean pulmonary artery pressure(PAMP)was measured in 3 groups. The arteria mesenterica smooth muscle cell capacitance of membrane(Cm), potassium current, and the current after BKCa was blocked by tetraethylammonium(TEA)were recorded by whole cell patch clamp and the BKCa current and the BKCa current density were calculated. The BKCa protein expression was measured by immuno-histochemical stain. RESULTS: Compared with Group B, the PAMP was lower than that in Group A or Group C(P <0.01), the membrane capacitance, BKCa current, current density and iOD value in Group A were higher(P <0.05), the BKCa current, BKCa current density were increased at different degrees in Ggroup C (P <0.05), but there was no change in iOD value. CONCLUSION: Valsartan can effectively reduce pulmonary pressure in hypoxia- pulmonary artery hypertension rats. The mechanism was probably the BKCa was activiated by valsartan.

Key words: valsartan, large-conductance calciumactivated potassium channel, patch clamp technique, pulmonary artery hypertension

中图分类号:

R965.2

王新, 杨玉雯, 王安才, 孔祥权. 缬沙坦干预对缺氧性肺动脉高压大鼠大电导钙激活钾通道的影响[J]. 中国临床药理学与治疗学, 2009, 14(9): 1032-1035.

WANG Xin, YANG Yu-wen, WANG An-cai, KONG Xiang-quan. Effects of valsartan on BKCa of hypoxic pulmonary hypertensive rats[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2009, 14(9): 1032-1035.

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