四逆散冻干粉血清移行成分的分析研究

中国临床药理学与治疗学 ›› 2010, Vol. 15 ›› Issue (1): 21-26.

四逆散冻干粉血清移行成分的分析研究

李越峰¹, 李廷利²

¹甘肃中医学院, 兰州730000, 甘肃;
²黑龙江中医药大学, 哈尔滨150040, 黑龙江

收稿日期:2009-11-13 修回日期:2009-12-11 出版日期:2010-01-26 发布日期:2020-09-21
通讯作者: 李廷利,男, 教授、博士生导师, 主要从事中药药物活性成分的研究。Tel: 0451-82196294
作者简介:李越峰, 女, 博士, 副教授, 研究方向是中药及复方药效物质基础及机制研究。Tel: 13619349364 E-mail: lyfyxk@126.com
基金资助:
国家自然科学基金资助项目(30672634)

Analysis study on the transmigration ingredient of blood serum of Sinisan Freeze-dry Powder

LI Yue-feng¹, LI Ting-li²

¹University of TCM of Gansu, Lanzhou 730000, Gansu, China;
²University of TCM of Heilong jiang, Haerbin 150040, Heilong jiang, China

Received:2009-11-13 Revised:2009-12-11 Online:2010-01-26 Published:2020-09-21

摘要/Abstract

摘要： 目的研究四逆散冻干粉血清移行成分, 为确定四逆散改善睡眠作用药效物质基础提供可靠的方法依据。方法采用HPLC 方法, 梯度洗脱,测定了四逆散冻干粉血清移行成分。色谱条件为:HypersilC18柱(4 .6 mm ×250 mm, 5 μm), 流速1 .0 mL/min, 柱温30 ℃, 流动相A 为乙腈;流动相B 为0 .05 %磷酸水, 流动相A 梯度洗脱(10 %~ 90 %乙腈), 分析时间为150 min 。结果四逆散冻干粉在连续给药7 d 后血清中出现14种移行成分。结论四逆散血清HPLC 指纹图谱特征性及专属性强, 可用于四逆散冻干粉血清移行成分的分析研究。

关键词: 四逆散冻干粉, 血清移行成分, 指纹图谱

Abstract: AIM: To study the transmigration ingredient of blood serum of Sinisan Freeze-dry Powder in order to determine that Sinisan enhances the sleep function with drug effect material base. METHODS: The HPLC method was used to do gradient elution, and the transmig ration ingredient of blood serum of Sinisan Freezedry Pow der was determined.The chromatograph condition: Hypersil C18 column (4.6 mm×250 mm, 5 μm), speed of flow 1.0 mL/min, column warm 30 ℃, flowing A is acetonit rile ;flowing B is 0.05 % phosphorus acid water, the flow ingA gradient elution (10 %-90 % aceto nitrile), the analysis time is 150 min.RESULTS: 14 kinds of transmig rationing redient sappeared in blood serum after seven days successive medication with Sinisan Freeze-dry Powder.CONCLUSION: The specificity and characteristic of the blood serum HPLC fingerprint mark in Sinisan are good, which can be used in the transmig ration ingredient of blood serum of Sinisan Freeze-dry Powder.

Key words: Sinisan Freeze-dry Powder, Transmigration ingredient of blood serum, Fingerprinting

中图分类号:

R969.1

李越峰, 李廷利. 四逆散冻干粉血清移行成分的分析研究[J]. 中国临床药理学与治疗学, 2010, 15(1): 21-26.

LI Yue-feng, LI Ting-li. Analysis study on the transmigration ingredient of blood serum of Sinisan Freeze-dry Powder[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2010, 15(1): 21-26.