三种中药活性部位对H2O2致PC12细胞损伤保护作用的比较研究

中国临床药理学与治疗学 ›› 2010, Vol. 15 ›› Issue (1): 53-58.

三种中药活性部位对H₂O₂致PC12细胞损伤保护作用的比较研究

胡涛¹, 刘晓岩¹, 郭宏宝², 王银叶¹

¹北京大学药学院分子与细胞药理学系, ²北京大学药学院应用药物研究所, 北京100191

收稿日期:2009-09-30 修回日期:2009-12-15 出版日期:2010-01-26 发布日期:2020-09-21
通讯作者: 王银叶, 女, 教授, 硕士生导师, 研究方向:心血管药理学。Tel:010-82802652 　E-mail: wangyinye@bjmu.edu.cn
作者简介:胡涛, 男, 硕士, 研究方向: 心血管药理学。E-mail: 1985hutao@gmail.com

Comparative study on neuroprotective effects of active componants of several traditional Chinese medicines on H₂O₂-induced PC12 cells injury

HU Tao¹, LIU Xiao-yan¹, GUO Hong-bao², WANG Yin-ye¹

¹Department of Molecular and Cellular Pharmacology, ²Institute of Application Medicine, School of Pharmaceutical Sciences, Peking University, Beijing 100191, China

Received:2009-09-30 Revised:2009-12-15 Online:2010-01-26 Published:2020-09-21

摘要/Abstract

摘要： 目的比较三种中药有效部位人参皂苷、葛根黄酮和麦冬皂苷对H2O2 诱导的PC12 细胞氧化损伤的保护作用。方法用H₂O₂ 诱导PC12细胞氧化损伤, 用MT T 法测定细胞的存活率, 黄嘌呤氧化酶法测定培养液中超氧化物歧化酶(SOD) 活性, 硫代巴比妥酸法测定培养液中丙二醛(MDA) 含量, Griess 法测定培养液中NO 含量。结果在人参皂苷、葛根黄酮、麦冬皂苷三种中药有效组分中, 葛根黄酮对H₂O₂ 氧化损伤的PC12 细胞的保护作用最强, 人参皂苷和麦冬皂苷较弱, 且强度接近。葛根黄酮提高SOD 活性的活性最强, 麦冬皂苷次之, 人参皂苷较弱。降低MDA 的作用强度顺序为葛根黄酮>人参皂苷>麦冬黄酮。葛根黄酮减少NO 生成的作用远强于人参皂苷, 麦冬皂苷无此作用。结论人参皂苷、葛根黄酮、麦冬皂苷均可保护PC12 细胞免受H₂O₂ 的氧化损伤, 该作用的强弱与它们提高SOD 活性、减少脂质过氧化和NO 产生的作用强弱有关。葛根黄酮的作用在三种有效组分中最强。

关键词: PC12 细胞, 过氧化氢, 人参皂苷, 葛根黄酮, 麦冬皂苷

Abstract: AIM: To compare the protective effects of several traditional Chinese medicines ginsenosides, puerariaiso flavones and ophiopo gonis on H₂O₂-induced PC12 cell injury.METHODS: Cells were treated with vehicle, series concentrations of ginsenosides, puerariaiso flavones or ophiopogonis, then were injured with H₂O₂.The viability of PC12 cells was determined by MTT assay ;SOD level in culture medium was determined by xanthinoxidanse method ;MDA content in medium was measured by modified thiobarbituric acid (TBA) method and NO content in culture medium was determined by Griessme thod.RESULTS: Among these traditional Chine se medicine components, pueraria iso flavones showed the most potent neuroprotective effects, ophiopogonis and ginsenosides showed the almost equal potency. Puerariaiso flavones displayed the most potent effect on the increasing SOD production, ophiopogonis was less effective, and ginsenosides was weakest on this action.Puerariaiso flavones most potently reduced MDA and NO content, ginseno sides showed better effect compared with ophiopogonis.Whereas ophiopogonis failed to reduce NO production.CONCLUSION: Ginsenosides, pueraria is of lavones and ophiopogonis all show signif icant pro tective effect son H2O2-induced PC12 cells injury, and the intensity of this effect may result from their potency on the increase of SOD level and the decrease of lipid peroxidation and NO production.Puerariais of lavones is most potent among three components.

Key words: PC12 cell, H₂O₂, Ginsenosides, Ppueraria isoflavones, Ophiopogonis

中图分类号:

R967

胡涛, 刘晓岩, 郭宏宝, 王银叶. 三种中药活性部位对H₂O₂致PC12细胞损伤保护作用的比较研究[J]. 中国临床药理学与治疗学, 2010, 15(1): 53-58.

HU Tao, LIU Xiao-yan, GUO Hong-bao, WANG Yin-ye. Comparative study on neuroprotective effects of active componants of several traditional Chinese medicines on H₂O₂-induced PC12 cells injury[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2010, 15(1): 53-58.

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三种中药活性部位对H₂O₂致PC12细胞损伤保护作用的比较研究

Comparative study on neuroprotective effects of active componants of several traditional Chinese medicines on H₂O₂-induced PC12 cells injury

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