一种新的半数效量测定方法——简化随机逼近法

中国临床药理学与治疗学 ›› 2010, Vol. 15 ›› Issue (10): 1139-1142.

一种新的半数效量测定方法——简化随机逼近法

聂莉, 潘伟, 宋建国

皖南医学院药理学教研室,芜湖 241002,安徽

收稿日期:2010-07-02 修回日期:2010-10-18 发布日期:2020-09-16
通讯作者: 宋建国,男,博士,教授,研究方向:数学药理学。
作者简介:聂莉,女,硕士在读,研究方向:时间药理学。Tel: 15212234929 E-mail: nlwnmc@126.com

A new method for median effective dosage measurement--improved robbins-monro process

NIE Li, PAN Wei, SONG Jian-guo

Department of Pharmacology, Wannan Medical College, Wuhu 241002, Anhui, China

Received:2010-07-02 Revised:2010-10-18 Published:2020-09-16

摘要/Abstract

摘要： 目的: 本文根据序贯法原理设计了一种新的测定药物半数效量的方法:简化随机逼近法。并初步探讨了该法的可靠性和优缺点。方法: 采用简化随机逼近法测定了普鲁卡因和戊四氮腹腔注射对小鼠的LD₅₀,并与经典的Bliss法进行比较。结果: 简化随机逼近法测得的普鲁卡因和戊四氮的LD₅₀分别为 207.5 mg/kg、107.2 mg/kg,而Bliss法测得的LD₅₀分别为 206.1 mg/kg 和 101.2 mg/kg。两法测定结果相近,但简化随机逼近法更为省时、省动物且计算简便。结论: 简化随机逼近法适用于药效反应快、终点判定所需时间短的药物LD₅₀或ED₅₀的测定。

关键词: 简化随机逼近法, Bliss法, LD₅₀, 普鲁卡因, 戊四氮

Abstract: AIM: A new method to measure median effective dosage based on the principle of sequential trial was designed: improved robbins-monro process. To explore the reliability, advantage and disadvantage of the method. METHODS: Median lethal doses(LD₅₀) of procaine and pentetrazole were detected and compared with improved robbins-monro process and Classical Bliss method. RESULTS: LD₅₀ of procaine and pentetrazole with the improved robbins-monro process were 207.5 mg/kg and 107.2 mg/kg respectively, LD₅₀ with the Bliss method were 206.1 mg/kg and 101.2 mg/kg respectively. Results indicated that the LD₅₀ with improved robbins-monro process is similar with Bliss method, while the new method is fewer animals and time consuming and simpler to calculate. CONCLUSION: Improved robbins-monro process is suitable to mesure median effective dose, for example LD₅₀ or ED₅₀, for the drug which efficicy is rapid and point is easy to determine the end.

Key words: Improved robbins-monro process, Bliss method, Median lethal dose (LD₅₀), Procaine, Pentetrazole

中图分类号:

R311

聂莉, 潘伟, 宋建国. 一种新的半数效量测定方法——简化随机逼近法[J]. 中国临床药理学与治疗学, 2010, 15(10): 1139-1142.

NIE Li, PAN Wei, SONG Jian-guo. A new method for median effective dosage measurement--improved robbins-monro process[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2010, 15(10): 1139-1142.