哮喘大鼠ERKmRNA及磷酸化蛋白水平的动态变化

中国临床药理学与治疗学 ›› 2010, Vol. 15 ›› Issue (2): 166-169.

哮喘大鼠ERKmRNA及磷酸化蛋白水平的动态变化

张近波¹, 吴成云², 李智强³, 戴元荣²

¹温州医学院附属温岭医院EICU, 温岭 317500,浙江;
²温州医学院附属第二医院呼吸内科, ³温州医学院附属第一医院ICU,温州 325000,浙江

收稿日期:2009-11-09 修回日期:2009-12-11 出版日期:2010-02-26 发布日期:2020-09-18
通讯作者: 吴成云,男,硕士研究生,副主任医师,主要从事慢性阻塞性肺疾病及支气管哮喘方面的研究。E-mail:wcyzxqdd@hotmail.com
作者简介:张近波,男,硕士研究生,医师,主要从事危重病研究。E-mail: zhangjinbo0661@163.com
基金资助:
温州市科学技术局资助项目(Y2006A036)

Dynamic change of ERKmRNA and phosphorylation protein level in rats with asthma

ZHANG Jin-bo¹, WU Cheng-yun², LI Zhi-qiang³, DAI Yuan-rong²

¹ Departnent of EICU,the Wenling Hospital Affiliated to Wenzhou Medical College, Wenling 317500,Zhejiang, China;
² Departnent of Respiratory Medicine,the Second Affiliated Hospital Wenzhou Medical College, ³Departnent of ICU,the First Affiliated Hospital Wenzhou Medical College,Wenzhou 325000,Zhejiang,China

Received:2009-11-09 Revised:2009-12-11 Online:2010-02-26 Published:2020-09-18

摘要/Abstract

摘要： 目的:探讨哮喘大鼠ERKmRNA及磷酸化蛋白水平的动态变化。方法:分离培养正常对照组、哮喘组(分成哮喘 0.5、1、3、12 h 组)大鼠的外周血单个核细胞(PBMCs)。分别采用RT-PCR、Western blotting技术检测各组PBMCs ERKmRNA、ERK磷酸化蛋白的表达。结果:哮喘(0.5、1、3 h)组PBMCs ERKmRNA、ERK磷酸化蛋白表达水平较正常对照组显著升高(均P＜0.01),哮喘(12 h)组和正常对照组比较差异无统计学意义(P>0.05)。哮喘(1 h)组ERKmRNA、ERK磷酸化蛋白表达水平较正常对照组和哮喘(0.5、3、12 h)组均显著升高(均P＜0.01) 。结论:磷酸化ERK可能在哮喘气道炎症和重塑中发挥重要作用, 深入研究ERK在哮喘发病机制中的具体调控作用,对临床支气管哮喘的防治可能有重要意义。

关键词: 哮喘, 外周血单个核细胞, 细胞外信号调节激酶

Abstract: AIM: To explore the dynamic changes of ERKmRNA and phosphorylation protein level in rats with asthma. METHODS: The rats were divided into control group,asthma (0.5,1,3,12 h)groups and the peripheral blood mononuclear cells(PBMCs)of rats were cultured and collected. The expressions of ERKmRNA and phosphor protein of ERK in PBMCs of all groups were detected by RT-PCR,Western bloting, respectively. RESULTS: Compared with control group,the expressions of ERKmRNA,phosphor protein of ERK in PBMCs were increased in asthma (0.5,1,3 )groups (all P<0.01). There was no difference between asthma(12 h)and control group. Compared with control group,asthma (0.5,3,12 h)groups,the expressions of ERKmRNA,phosphor protein of ERK in PBMCs were increased in asthma(1 h)group. CONCLUSION: Phosphorylation ERK may play an important role in airway inflammation and remodeling of asthma,deeply studying the ERK's specific regulation results in the pathogenesis of asthma may have an important significance on the prevention and treatment clinical asthma.

Key words: Asthma, Peripheral blood mononuclear cells

中图分类号:

R965.2

张近波, 吴成云, 李智强, 戴元荣. 哮喘大鼠ERKmRNA及磷酸化蛋白水平的动态变化[J]. 中国临床药理学与治疗学, 2010, 15(2): 166-169.

ZHANG Jin-bo, WU Cheng-yun, LI Zhi-qiang, DAI Yuan-rong. Dynamic change of ERKmRNA and phosphorylation protein level in rats with asthma[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2010, 15(2): 166-169.

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