降糖复方对脂性肝细胞损伤与高脂血症的保护作用

中国临床药理学与治疗学 ›› 2010, Vol. 15 ›› Issue (3): 260-265.

降糖复方对脂性肝细胞损伤与高脂血症的保护作用

巫冠中¹, 章娟², 黄维佳³

¹中国药科大学药理教研室, 南京210009, 江苏;
²上海复旦大学附属肿瘤医院药剂科, 上海200032;
³北京解放军医学出版社, 北京100036

收稿日期:2009-09-21 修回日期:2009-09-21 发布日期:2020-10-14
通讯作者: 章娟, 女, 硕士, 研究方向:内分泌药理。Tel:13918598079 E-mail:zhangjuan1006@yahoo.com.cn
作者简介:巫冠中, 男, 研究方向:内分泌药理。E-mail:zhonghuw@sohu.com

Protective effects of Jiangtang compound recipe on injured-steatosishepatocyte and hyper lipemia

WU Guan-zhong¹, ZHANG Juan², HUANG Wei-jia³

¹Department of Pharmacology, China Pharmaceutical University, Nanjing 210009, Jiangsu, China;
²Department of Pharmacy, Cancer Hospital Affiliated of Shanghai Fudan University, Shanghai 200032, China;
³P LA Med ical PUbl ishing House, Bei jing 100036, China

Received:2009-09-21 Revised:2009-09-21 Published:2020-10-14

摘要/Abstract

摘要： 目的: 研究降糖复方对脂肪酸诱导的HepG2 细胞脂性损伤与鹌鹑高脂血症的保护作用。方法: 采用高脂饲料诱导鹌鹑实验性高脂血症模型以及采用棕榈酸与油酸混合脂肪酸诱导HepG2 细胞发生脂肪损伤变性, 结合含药血清学, 检测细胞与血清中TG、丙二醛(MDA)含量、超氧化物歧化酶(SOD)活力, 以及血清中高密度脂蛋白胆固醇(HLD-C)含量;油红O 染色, 观察细胞内脂质聚集情况;检查鹌鹑动脉内膜病理变化。结果: 降糖复方明显减少高血脂鹌鹑动脉内膜粥样硬化斑块和抑制内膜病变程度, 降低HepG2 细胞内脂质堆积, 降低细胞与血清中TG、MDA 的含量, 并调节SOD 活性。结论: 降糖复方显著抑制细胞的脂质蓄积, 减轻鹌鹑高脂血症和动脉粥样斑块的形成。

关键词: 降糖复方, HepG2, 脂性肝细胞损伤, 鹌鹑

Abstract: AIM: To study the protective effects of Jang tang compo und recipe on injuredsteato sis hepatocyte of HepG2 cells invtiro and hyperlipemia of quails in vivo. METHODS: The hy perlipemia model of quails was induced by high fat diet in vivo and the content oftrigly ceride (TG), hig h density lipoprotein-cholesterol (HDL-C), malondialdehyde(MDA), activity of supero xide dismutase (SOD)in serums were assayed as well as patholo gical change of vascular endarterium detected. Further, the injuredsteatosis model of HepG2 cells induced with mixture fat ty acids mixed palmi tic and oleic (PA∶OA =1∶2)wast reated with vario us medicinal serum and TG, MDA, SOD activity in supernatant determined.The treated cells were al so stained with oil-redO to observe the lipid accumulation. RESULTS: The Jang tang compound recipe sig nificantly reduced intima athero scle ro tic plaque area of quails suf fered from hype rlipemia and inhibited intimal lesion. It al so inhibited lipid accumulation in HepG2 cells, reduced the content of TG, MDA and adjust the activity of SOD. CONCLUSION: Jang tang compound recipe significantly inhibits the fatty accumulation in HepG2 cells, alleviates die t-induced hyperlipidemia and the formation of atheroasclerosis in quails.

Key words: Jangtang compound recipe, HepG2, Steatosis hepatocyte, Quails

中图分类号:

R965.2

巫冠中, 章娟, 黄维佳. 降糖复方对脂性肝细胞损伤与高脂血症的保护作用[J]. 中国临床药理学与治疗学, 2010, 15(3): 260-265.

WU Guan-zhong, ZHANG Juan, HUANG Wei-jia. Protective effects of Jiangtang compound recipe on injured-steatosishepatocyte and hyper lipemia[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2010, 15(3): 260-265.

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