安妥沙星纸片扩散法外抗菌活性测定折点初步研究

中国临床药理学与治疗学 ›› 2010, Vol. 15 ›› Issue (3): 298-304.

安妥沙星纸片扩散法外抗菌活性测定折点初步研究

肖永红, 李耘, 刘健, 钟巍, 杨维维

北京大学第一医院临床药理研究所, 北京100191

收稿日期:2009-12-09 修回日期:2009-12-09 发布日期:2020-10-14

Primary study of antofloxaicn breakpoints with disc-diffusion sensitivity test

XIAO Yong-hong, LI Yun, LIU Jian, ZHONG Wei, YANG Wei-wei

Inst itute of Cl inical P harmacology, First Hospital, Peking University, Beijing 100191, China

Received:2009-12-09 Revised:2009-12-09 Published:2020-10-14

摘要/Abstract

摘要： 目的: 确定安妥沙星对葡萄球菌属、肠杆菌科、非发酵菌及嗜血杆菌属的纸片扩散法体外抗菌活性测定折点。方法: 采用标准琼脂二倍稀释法与纸片扩散法(5μg 和10μg) 测定安妥沙星对临床常见致病菌的敏感性, 并与临床常用的氟喹诺酮类药物相比较分析, 结合人体药代动力学参数, 利用MIC 与抑菌圈直径散点图, 初步确定安妥沙星纸片扩散法对常见细菌的折点。结果: 安妥沙星的体外抗菌作用与左氧氟沙星接近且相关性最好, 根据安妥沙星琼脂稀释法体外抗菌活性测定的临界浓度(嗜血杆菌敏感临界浓度为≤1mg/ L, 其他细菌敏感、中介与耐药临界浓度分别为≤2、4、≥8 mg/ L), 利用MIC 与抑菌圈直径散点图初步确定安妥沙星(10 μg) 纸片对嗜血杆菌抑菌圈直径≥21 mm 为敏感, 其它菌种耐药、中介与敏感的抑菌圈直径分别为≤14 mm、15 ~17 mm 和≥18 mm。标准菌株质控范围分别为大肠埃希菌ATCC25922 24~31 mm, 铜绿假单胞菌ATCC27853 22~26 mm, 金黄色葡萄球菌ATCC25923 22~28 mm。结论: 通过体外抗菌活性比较, 利用MIC 与抑菌圈直径散点图, 初步确定了安妥沙星纸片扩散法体外抗菌活性测定对常见细菌的折点, 供临床应用参考与验证。

关键词: 安妥沙星, 折点, 最小抑菌浓度, 抑菌圈, 纸片扩散法

Abstract: AIM: To determine the breakpoints of Antofloxacin sensitivity with disc-diffusion test for staphylococci, enterobacteriaceae, non-fermenter and hemophili in primary. METHODS: The in vitro antibacterial activity of Antofloxacin and other comparators was detected with standard agar dilution and disc-diffusion (Antofloxacin 5 μg and 10 μg discs) methods. Referring to the association of in vitro antibacterial activity of different fluoroquinolones and pharmacokinetics property of the investigated agent, the breakpoints of Antofloxacin were determined with scat tergram between MIC and bacterial inhibitory zone. RESULTS: The in vitro antibacterial activity of Antofloxacin against clinical isolates shown better association with those of levof loxacin. Referring to the proposed sensitive breakpoints of Antofloxacin with agardilution method (hemophili being recommended as ≤1 mg/L for sensitive the sensitive, intermediate and resistant breakpoint sagainst other pathogens being≤2, 4, ≥8 mg/L, respectively), the bacterial inhibitory zone breakpoints with disc-diffusion method (10μg disc) were setas ≥21 mm against hemophili for sensitive and ≤14 mm, 15-17 mm, ≥18 mm against the other germs for resistant, intermediate and sensitive. The quality control inhibitory zones against referring strains were 24-31mm for Escherichia coli A TCC25922, 22-26 mm for Pseudomonas aeruginosa A TCC27853 and 22-28mm for Staphylococcus aureus ATCC25923, respectively. CONCLUSION: Referring to the in vitro antibacterial activity and making use of the scatter gram between MIC and inhibition zone of Antofloxacin, the breakpoints for in vitro sensitive test with disc-diffusion method were established in primary and recommended for clinical practice and future review.

Key words: Anto flo xacin, Breakpoint, Minimum inhibitory concentration, Disc-diffusiontest, Bacterial inhibi to ryzone

中图分类号:

R965.2

肖永红, 李耘, 刘健, 钟巍, 杨维维. 安妥沙星纸片扩散法外抗菌活性测定折点初步研究[J]. 中国临床药理学与治疗学, 2010, 15(3): 298-304.

XIAO Yong-hong, LI Yun, LIU Jian, ZHONG Wei, YANG Wei-wei. Primary study of antofloxaicn breakpoints with disc-diffusion sensitivity test[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2010, 15(3): 298-304.

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