银杏叶提取物对TGF-β1诱导的人Tenon's囊成纤维细胞增殖的影响及其机制的初步探讨

中国临床药理学与治疗学 ›› 2011, Vol. 16 ›› Issue (12): 1347-1351.

银杏叶提取物对TGF-β₁诱导的人Tenon's囊成纤维细胞增殖的影响及其机制的初步探讨

凌佼佼, 李平华

重庆医科大学附属第一医院眼科, 眼科学重庆市市级重点实验室,重庆 400016

收稿日期:2011-09-16 修回日期:2011-11-15 出版日期:2011-12-26 发布日期:2012-01-07
通讯作者: 李平华, 通信作者,男,教授,硕士生导师,研究方向:青光眼,白内障。E-mail: cylipinghua@163.com
作者简介:凌佼佼,女,硕士研究生, 研究方向:青光眼,白内障。Tel: 15310626595 E-mail: ling19860515@sohu.com

Effects of Ginkgo biloba extract on cell proliferation in human Tenon's capsule fibroblasts stimulated by TGF-β₁ and its potential mechanism

LING Jiao-jiao, LI Ping-hua

Department of Opthalmology, the First Affiliated Hospital, Chongqing Medical University, Chongqing Key Laboratory of Ophthalmology, Chongqing 400016, China

Received:2011-09-16 Revised:2011-11-15 Online:2011-12-26 Published:2012-01-07

摘要/Abstract

摘要： 目的: 观察银杏叶提取物(GBE)对转化生长因子-β₁(TGF-β₁)诱导的人Tenon's囊成纤维细胞(HTCFs)增殖的影响及可能机制。方法: 体外培养HTCFs及鉴定; MTT法检测不同浓度的GBE(25、50、100、200 mg/L)对TGF-β₁(2 ng/mL)诱导的HTCFs增殖的影响;流式细胞仪检测细胞周期;免疫细胞化学法观察α-平滑肌动蛋白(α-SMA)表达的情况。RT-PCR检测细胞结缔组织生长因子mRNA(CTGF mRNA)的表达。结果: TGF-β₁可显著促进HTCFs的增殖,促进细胞由G₀/G₁期进入S期,上调α-SMA、CTGF在蛋白和mRNA水平的表达;GBE能够抑制TGF-β₁的上述作用。结论: GBE具有抑制TGF-β₁诱导的人Tenon's囊成纤维细胞增殖的作用, 其机制可能是阻止细胞进入S期,下调α-SMA及CTGF的表达。

关键词: 银杏叶提取物, 人Tenon's囊成纤维细胞, 转化生长因子-β₁, α-平滑肌动蛋白, 结缔组织生长因子

Abstract: AIM: To investigate the effects of Ginkgo biloba extract on cell proliferation in cultured human Tenon's capsule fibroblasts and its potential mechanism.METHODS: Human Tenon's capsule fibroblasts were cultured in vitro, the subcultured cells were identified by morphology observation and Vimentin staning. Untreated cells were acted as normal control group,TGF-β₁ as the stimulating factor, the cells were treated with GBE with concentrations of 0,25,50,100 and 200 mg/L. Cell proliferation was observed by MTT assay, flow cytometry tested cell cycle, the expression of α-smooth muscle-actin (α-SMA) was detected by immunocytochemistry, the mRNA level of connective tissue growth factor (CTGF) was measured by RT-PCR.RESULTS: TGF-β₁ markedly increased the cell proliferation, GBE effectively inhibited the proliferation in a concentration-time dependent way. The S phase of cells in TGF-β₁ group were gradually increased, the expression of α-SMA and CTGF were also markedly increased induced by TGF-β₁, which was partly attenuated by GBE.CONCLUSION: Ginkgo biloba extract can inhibit HTCFs proliferation and down regulation the TGF-β₁ induced α-SMA and CTGF mRNA overexpression.

Key words: Ginkgo biloba extract, Human Tenon's capsule fibroblasts, Transforming growth factor-β₁, α-smooth muscle-actin, Connective tissue growth factor

中图分类号:

R965.2

凌佼佼, 李平华. 银杏叶提取物对TGF-β₁诱导的人Tenon's囊成纤维细胞增殖的影响及其机制的初步探讨[J]. 中国临床药理学与治疗学, 2011, 16(12): 1347-1351.

LING Jiao-jiao, LI Ping-hua. Effects of Ginkgo biloba extract on cell proliferation in human Tenon's capsule fibroblasts stimulated by TGF-β₁ and its potential mechanism[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2011, 16(12): 1347-1351.

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银杏叶提取物对TGF-β₁诱导的人Tenon's囊成纤维细胞增殖的影响及其机制的初步探讨

Effects of Ginkgo biloba extract on cell proliferation in human Tenon's capsule fibroblasts stimulated by TGF-β₁ and its potential mechanism

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