C-反应蛋白基因多态性与代谢综合征的关联研究

中国临床药理学与治疗学 ›› 2011, Vol. 16 ›› Issue (12): 1391-1396.

C-反应蛋白基因多态性与代谢综合征的关联研究

金岳龙¹, 贺连平¹, 陈金凤², 王彬彬³, 沈冲², 姚应水¹

¹皖南医学院预防医学系,芜湖 241002,安徽;
²南京医科大学公共卫生学院流行病与卫生统计学系,南京 210029,江苏;
³南京医科大学第一临床医学院,南京 210029,江苏

收稿日期:2011-10-11 修回日期:2011-11-15 出版日期:2011-12-26 发布日期:2012-01-07
通讯作者: 姚应水,通信作者,男,硕士,教授,硕士生导师,研究方向:慢性病分子流行病学。E-mail: yingshuiyao@163.com
作者简介:金岳龙、贺连平为共同第一作者。金岳龙,男,硕士,讲师,研究方向:慢性病分子流行病学。贺连平,男,在读硕士,研究方向:慢性病分子流行病学。
基金资助:
国家自然科学基金(81072367);安徽省自然科学基金(090413126)

Association of polymorphism in CRP gene with metabolic syndrome

JIN Yue-long¹, HE Lian-ping¹, CHEN Jin-feng², WANG Bin-bin³, SHEN Chong², YAO Ying-shui¹

¹Department of Preventive Medicine, Wannan Medical College, Wuhu 241002, Anhui, China;
²Department of Epidemiology & Statistics, School of Public Health, Nanjing Medical University,Nanjing 210029,Jiangsu,China;
³The First Clinical Medical College, Nanjing Medical University, Nanjing 210029,Jiangsu,China

Received:2011-10-11 Revised:2011-11-15 Online:2011-12-26 Published:2012-01-07

摘要/Abstract

摘要： 目的: 探讨C-反应蛋白(C-reaction protein,CRP)基因rs6677719位点多态性与代谢综合症(metabolism syndrome,MS)的关系。方法: 研究对象来自从某乡镇6个村整群抽样调查人群,纳入301例MS对象作为病例组,同时选取年龄和性别与病例匹配的547例健康个体作为对照组,所有的研究对象完成了问卷调查、体格检查及临床生化指标检测。采用聚合酶链反应-限制性片段多态性(PCR-RFLP)方法完成CRP基因rs6677719位点基因分型,比较不同基因型在病例组和对照组中的分布差异,及其与血糖、血压和血脂等指标的关系。结果: 关联分析结果表明,rs6677719 位点变异与MS存在显著关联,相加模型和显性模型均有统计学意义,OR值(95%可信区间)分别为0.785(0.640~0.862)和0.659(0.489~0.889)。对照组TC基因型TG水平显著低于TT基因型(P<0.05),而高密度脂蛋白(HDL-C)和低密度脂蛋白(LDL-C)水平在TT、TC和CC基因型间存在显著线性关系(P<0.05)。结论: CRP基因rs6677719位点变异与MS存在显著关联,并影响人体TG、HDL-C和LDL-C代谢。

关键词: 代谢综合征, C-反应蛋白, 关联研究

Abstract: AIM: To investigate the association of rs6677719 polymorphism in the C-reactive protein gene with metabolic syndrome.METHODS: Stratified cluster sampling method was conducted to select 301 cases of metabolic syndrome and 547 healthy individuals who were matched with cases for age and gender from a community based population.Questionnaire was managed, and physical examination and blood biomarkers tests were conducted, and rs6677719 locus was detected by the method of polymerase chain reaction-restriction fragment length polymorphism (RFLP).The distributions of TT, TC and CC genotype frequencies between cases and controls were compared and the association of rs6677719 polymorphism with serum glucose, blood pressure and blood lipids were analyzed.RESULTS: The results of association analysis indicated that rs6677719 variation had significant correlation with MS, and additive and dominant models presented statistical significances, odds ratios (OR) and corresponding 95% confidence interval (CI) were 0.785 (0.640-0.862) and 0.659 (0.489-0.889) ,respectively.The TG level of TC genotype carrier was significantly lower than that of TT genotype carrier in control group.Furthermore, the HDL-C and LDL-C levels of individuals presented significant linear trend in TT, TC and CC genotypes.CONCLUSION: The results in the present study suggest that rs6677719 polymorphism in CRP gene is associated with MS and the genetic variation significantly affects the TG, HDL-C and LDL-C of metabolisms in general population.

Key words: Metabolic syndrome, C-reactive protein, Association study

中图分类号:

R968

金岳龙, 贺连平, 陈金凤, 王彬彬, 沈冲, 姚应水. C-反应蛋白基因多态性与代谢综合征的关联研究[J]. 中国临床药理学与治疗学, 2011, 16(12): 1391-1396.

JIN Yue-long, HE Lian-ping, CHEN Jin-feng, WANG Bin-bin, SHEN Chong, YAO Ying-shui. Association of polymorphism in CRP gene with metabolic syndrome[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2011, 16(12): 1391-1396.

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