Ca2+/PKC通路在大鼠缺氧复氧心肌细胞Egr-1高表达中的作用

中国临床药理学与治疗学 ›› 2011, Vol. 16 ›› Issue (3): 241-248.

• 基础研究 • 下一篇

Ca²⁺/PKC通路在大鼠缺氧复氧心肌细胞Egr-1高表达中的作用

郑付春¹, 贾强用², 石刚刚²

¹汕头大学医学院第一附属医院药剂科
²汕头大学医学院药理教研室,汕头 515041, 广东

收稿日期:2011-02-10 修回日期:2011-02-25 出版日期:2011-03-26 发布日期:2011-05-18
通讯作者: 石刚刚,男,博士,教授,博士生导师,研究方向:心血管药理及药物开发。Tel: 0754-88566782 E-mail: ggshi@stu.edu.cn
作者简介:郑付春,女,本科,主管药师,研究方向:心血管药理。Tel: 0754-88905491 E-mail: zhengfuchunsh@yahoo.com.cn
基金资助:
国家自然科学基金-广东联合基金(U0932005);中国高等教育博士点研究基金(200805600003)

Effects of Ca²⁺/PKC pathway on Egr-1 overexpression in hypoxia/reoxygenation cardiomyocytes of rats

ZHENG Fu-chun¹, JIA Qiang-yong², SHI Gang-gang²

¹Department of Pharmacy, 1st Affiliated Hospital, Shantou University Medical College;
²Department of Pharmacology, Shantou University Medical College, Shantou 515041, Guangdong,China

Received:2011-02-10 Revised:2011-02-25 Online:2011-03-26 Published:2011-05-18

摘要/Abstract

摘要： 目的: 观察Ca²⁺/PKC通路在大鼠心肌细胞缺氧复氧状态下参与Egr-1高表达。方法: 取生长5～6 d 的大鼠心肌细胞分为对照(Control)组、缺氧复氧(H/R)组、溶剂(DMSO)组、EGTA组、维拉帕米(VER)组、H7组和BIM组。各组心肌细胞制作H/R 模型。 RT-PCR法检测培养心肌细胞中Egr-1 mRNA的表达水平。 Western-blot法检测培养心肌细胞中Egr-1 蛋白的表达水平。结果: 与Control组相比,H/R组及DMSO组心肌细胞Egr-1 mRNA的表达水平明显增高(P<0.05);与H/R组相比,VER组、EGTA组、H7组、BIM组Egr-1 mRNA的表达明显下调,差异具有统计学意义(P<0.05)。与Control组相比,H/R组及DMSO组心肌细胞Egr-1蛋白表达水平明显增高(P<0.05);与H/R组相比,VER组、EGTA组、H7组、BIM组Egr-1蛋白的表达明显下调,差异具有统计学意义(P<0.05)。结论: 在大鼠心肌细胞缺氧复氧状态下,Ca²⁺/PKC信号通路参与介导了Egr-1的高表达。

关键词: Ca²⁺, 蛋白激酶C, Egr-1, 心肌细胞, 缺氧复氧

Abstract: AIM: To study effects of Ca²⁺/PKC pathway on Egr-1 overexpression in hypoxia/reoxygenation cardiomyocytes of rats.METHODS: Cultured cardiomyocytes of 5-6 day old rats were divided into control group, hypoxia/reoxygenation (H/R) group, DMSO group, EGTA group, Verapamil group, H7 group and BIM group. All groups of cardiomyocytes underwent the treatment of H/R, and then RT-PCR and Western blot were used to test expression of Egr-1 mRNA and Egr-1 protein respectively.RESULTS: There were high expression of Egr-1 mRNA in H/R group and DMSO group compared with control group (P<0.05). Verapamil, EGTA, H7 and Bim reduced the expression of Egr-1 mRNA significantly compared with H/R group (P<0.05). There were high expression of Egr-1 protein in H/R group and DMSO group compared with control group (P<0.05). Verapamil, EGTA, H7 and Bim reduced the expression of Egr-1 protein significantly compared with H/R group (P<0.05).CONCLUSION: Ca²⁺/PKC pathway plays a role on Egr-1 overexpression in hypoxia/reoxygenation cardiomyocytes of rats.

Key words: Ca²⁺, PKC, Early growth response gene-1, C & Cardiomyocytes, Hypoxia/reoxygenation

中图分类号:

R965.2

郑付春, 贾强用, 石刚刚. Ca²⁺/PKC通路在大鼠缺氧复氧心肌细胞Egr-1高表达中的作用[J]. 中国临床药理学与治疗学, 2011, 16(3): 241-248.

ZHENG Fu-chun, JIA Qiang-yong, SHI Gang-gang. Effects of Ca²⁺/PKC pathway on Egr-1 overexpression in hypoxia/reoxygenation cardiomyocytes of rats[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2011, 16(3): 241-248.

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Ca²⁺/PKC通路在大鼠缺氧复氧心肌细胞Egr-1高表达中的作用

Effects of Ca²⁺/PKC pathway on Egr-1 overexpression in hypoxia/reoxygenation cardiomyocytes of rats

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