肉苁蓉总苷对原代培养小鼠肝细胞酒精损伤保护作用研究

中国临床药理学与治疗学 ›› 2011, Vol. 16 ›› Issue (8): 856-861.

肉苁蓉总苷对原代培养小鼠肝细胞酒精损伤保护作用研究

罗慧英, 刘渊, 杨焕

甘肃中医学院药理教研室,甘肃省中药药理与毒理重点实验室,兰州 730000,甘肃

收稿日期:2011-06-29 修回日期:2011-08-08 出版日期:2011-08-26 发布日期:2011-09-07
作者简介:罗慧英,女,博士,副教授,硕士生导师。研究方向:保肝与抗肿瘤药物研究。Tel: 0931-8765395 E-mail: louria @vip.sina.com
基金资助:
甘肃省教育厅科研项目(0906B-01)

Protective effect of cistanchis glycosides on ethanol-induced damage in the primary hepatocytes of mice

LUO Hui-ying, LIU Yuan, YANG Huan

Department of Pharmacology, Gansu College of Traditional Chinese Medicine Lanzhou 730000, Gansu, China;Key Laboratory of Pharmacology and Toxicology for Traditional Chinese Medicines of Gansu Province, Lanzhou 730000, Gansu, China

Received:2011-06-29 Revised:2011-08-08 Online:2011-08-26 Published:2011-09-07

摘要/Abstract

摘要： 目的: 研究肉苁蓉总苷对原代培养肝细胞的保护作用。方法: 采用原位灌流法收集原代肝细胞,MTT法评价肉苁蓉总苷对酒精损伤原代培养肝细胞存活率的影响;荧光显微镜技术评价肉苁蓉总苷对酒精损伤原代培养肝细胞及细胞核形态的影响;流式细胞仪检测肉苁蓉总苷对酒精损伤原代培养肝细胞凋亡的影响;免疫细胞化学染色法检测肉苁蓉总苷对bcl-2和c-fos表达的影响。结果: 原代肝细胞经酒精损伤后,存活率降低,出现明显凋亡和坏死改变,凋亡抑制基因bcl-2减弱、促凋亡基因c-fos表达增强。肉苁蓉总苷可明显提高细胞存活率,改善凋亡和坏死情况,增强bcl-2表达,抑制c-fos表达。且作用呈剂量依赖性。结论: 肉苁蓉总苷可通过增加凋亡抑制基因bcl-2表达,减少促凋亡基因c-fos表达,减少凋亡和坏死,增加细胞存活率来实现对原代培养肝细胞的保护作用。

关键词: 肉苁蓉总苷, 原代培养, 肝细胞, 酒精性肝损伤, 凋亡

Abstract: AIM: To discuss the protection of cistanchis glycosides on ethanol-induced damage in the primary hepatocyte of mice.METHODS: The primary hepatocytes were collected by perfusion in situ method. The cell livability was evaluated by MTT, fluorescence microscopy and flow cytometry were used to evaluate the cell apoptosis and necrosis, immunohistochemistry was used to detect the expression of bcl-2 and c-fos.RESULTS: After the primary hepatocytes were damaged by alcohol,the cell livability decreased, apoptosis and necrosis were obvious,the expression of bcl-2 was decreased while that of c-fos was increased.Cistanchis glycosides could increase the cell livability, ameliorate apoptosis and necrosis, increase the expression of bcl-2, and decrease the expression of c-fos in a dose-dependent manner.CONCLUSION: The protective effects of cistanchis glycosides on ethanol-induced liver damage in the primary hepatocytes is associated with increasing the expression of bcl-2, and decreasing the expression of c-fos; resulting less apoptosis, less necrosis, and increasing the cell livability.

Key words: Cistanchis glycosides, Primary hepatocyte, Ethanol-induced liver damage, Apoptosis

中图分类号:

R965.2

罗慧英, 刘渊, 杨焕. 肉苁蓉总苷对原代培养小鼠肝细胞酒精损伤保护作用研究[J]. 中国临床药理学与治疗学, 2011, 16(8): 856-861.

LUO Hui-ying, LIU Yuan, YANG Huan. Protective effect of cistanchis glycosides on ethanol-induced damage in the primary hepatocytes of mice[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2011, 16(8): 856-861.

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