异丙酚对油酸型急性肺损伤兔血清TNF-α、IL-1β水平的影响

中国临床药理学与治疗学 ›› 2011, Vol. 16 ›› Issue (9): 1002-1005.

异丙酚对油酸型急性肺损伤兔血清TNF-α、IL-1β水平的影响

蒋柳明, 金建国, 祝卿, 吉伟, 林丽娜

温州医学院附属第一医院麻醉科,温州 325000,浙江

收稿日期:2011-05-14 修回日期:2011-08-28 出版日期:2011-09-26 发布日期:2011-10-11
通讯作者: 林丽娜,女,主任医师,教授,硕士生导师,研究方向:麻醉与器官保护。Tel: 0577-88069458 E-mail: wzlinlina@tom.com
作者简介:蒋柳明,男,硕士,研究方向:麻醉与器官保护。Tel: 0577-88069458 E-mail: a9713030b@163.com

Effect of propofol on serum TNF-α and IL-1β in rabbit with acute lung injury induced by oleic acid

JIANG Liu-ming, JIN Jian-guo, ZHU Qing, JI Wei, LIN Li-na

Department of Anesthesiology, the First Affiliated Hospital of Wenzhou Medical College, Wenzhou 325000, Zhejiang, China

Received:2011-05-14 Revised:2011-08-28 Online:2011-09-26 Published:2011-10-11

摘要/Abstract

摘要： 目的: 观察异丙酚对油酸性急性肺损伤(ALI)兔血清TNF-α、IL-1β水平的影响,探讨异丙酚治疗油酸型ALI的可能机制。方法: 健康日本大耳白兔24只,随机分成对照组(Ⅰ组)、急性肺损伤组(Ⅱ组)和异丙酚治疗组(Ⅲ组)。Ⅱ、Ⅲ组兔静脉注射油酸 0.08 mL/kg,复制日本大耳白兔ALI模型。建模成功后Ⅲ组静脉注射异丙酚 8 mg·kg^-1·h^-1,Ⅰ组和Ⅱ组静脉注射生理盐水 0.8 mL·kg^-1·h^-1至实验结束。各组大耳白兔分别在输入异丙酚或生理盐水前(T₀)和给药后 1 h(T₁)、2 h(T₂)、3 h(T₃)、4 h(T₄)采集动脉血用ELISA法检测血清TNF-α、IL-1β,同时进行血气分析,计算PaO₂／FiO₂结果: Ⅰ组血清中TNF-α、IL-1β浓度各时点无明显变化(P＞0.05)。Ⅱ组TNF-α浓度在T₁开始升高,T₂ 达高峰,T₃、T₄有下降但仍高于T₀ (P<0.05),与Ⅰ组相应时点比较升高明显(P<0.05)。Ⅲ组各时点 TNF-α浓度升高明显低于Ⅱ组(P<0.05)。与T₀比较,Ⅱ组血清IL-1β浓度在T₁开始升高,T₄达高峰(P<0.05),各时点浓度均明显高于Ⅰ组(P<0.05),Ⅲ组IL-1β浓度升高程度低于Ⅱ组(P<0.05)。Ⅰ组各时点氧合指数无明显变化。Ⅱ、Ⅲ组随着时间延长氧合指数逐渐下降,明显低于Ⅰ组(P<0.05),Ⅲ组氧合指数下降幅度较Ⅱ组低(P<0.05)。结论: 异丙酚能通过降低油酸性ALI兔血清TNF-α、IL-1β的释放,提高油酸性ALI后氧合指数,对兔油酸性ALI起到一定的保护效果。

关键词: 异丙酚, 急性肺损伤, 油酸, 肿瘤坏死因子-α, 白介素-1β, 氧合指数

Abstract: AIM: To observe the effects of propofol on serum TNF-α, IL-1β in rabbit with acute lung injury (ALI) induced by oleic acid (OA) and explore its possible protective mechanism and provide evidence for the choice of anesthesia drug in clinical anesthesia. METHODS: Twenty four health Japanese white rabbits were randomly divided into three groups (n=8): control group (group Ⅰ), model group (groupⅡ) and propofol treatment group (group Ⅲ). The rabbits in group Ⅱ and Ⅲ were injected oleic acid (OA) (0.08 mL/kg) by marginal veins of ear to establish ALI models. The rabbits of group Ⅲ were injected propofol (8 mg·kg^-1·h^-1) with pump, while group Ⅰ and group Ⅱ were injected normal saline (0.8 mL·kg^-1·h^-1) to end of the experiment after establishing the ALI models successfully. The concentration of TNF-α and interleukin-1β in serum were detected by ELISA at the points of propofol or normal saline infusion (T₀), 1 h after administration (T₁), 2 h after administration (T₂), 3 h after administration (T₃), 4 h after administration (T₄), and blood gas analysis of them had been performed to calculate the PaO₂/FiO₂ Ratio. RESULTS: The concentrations of TNF-α and interleukin-1β in serum in group Ⅰ had no difference at different points (P＞0.05). The concentration of TNF-α began to increase at T₁, reached to peak at T₂ and decreased at T₃, T₄, but still higher than those of T₀ (P<0.05). The concentrations of TNF-α in group Ⅱ increased more obviously than those of group Ⅰ(P<0.05) and were lower in group Ⅲ than those of group Ⅱ (P<0.05) at the corresponding time point. Compared with T₀ the concentrations of interleukin-1β in groupⅠbegan to increase at T₁, reached to the peak at T₄ (P<0.05) and were higher than those of group Ⅰ at the corresponding time points (P<0.05), increased lowerly in group Ⅲ than those of group Ⅱ(P<0.05). The oxygenation index at different time points had no significant change in group Ⅰ, decreased gradually after establishing the ALI models successfully in group Ⅱ and Ⅲ, were lower in group Ⅱ and Ⅲ than those of group Ⅰ (P<0.05), while decrease less in group Ⅱ than group Ⅲ. CONCLUSION: Propofol can significantly decrease the concentrations of serum TNF-α and IL-1β and obviously improve the oxygenation index of rabbits with ALI, has a possible protective effect of ALI induced by oleic acid.

Key words: Propofol, Acute lung injury, Oleic acid, TNF-α, IL-1β, Oxygenation index

中图分类号:

R965.2

蒋柳明, 金建国, 祝卿, 吉伟, 林丽娜. 异丙酚对油酸型急性肺损伤兔血清TNF-α、IL-1β水平的影响[J]. 中国临床药理学与治疗学, 2011, 16(9): 1002-1005.

JIANG Liu-ming, JIN Jian-guo, ZHU Qing, JI Wei, LIN Li-na. Effect of propofol on serum TNF-α and IL-1β in rabbit with acute lung injury induced by oleic acid[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2011, 16(9): 1002-1005.

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