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中国临床药理学与治疗学 ›› 2011, Vol. 16 ›› Issue (9): 992-997.

• 基础研究 • 上一篇    下一篇

葡萄籽原花青素对复发性结肠炎大鼠结肠组织中c-Jun、c-Fos表达的影响

王艳红1,2, 丁义兰3, 杨孝来2, 葛斌2, 路莉1, 张宝来1, 於洪建4, 刘丹4, 吴勇杰1   

  1. 1兰州大学基础医学院药理学研究所,兰州 730000,甘肃;
    2甘肃省人民医院 药剂科,兰州 730000,甘肃;
    3兰州市妇幼保健院,兰州 730030,甘肃;
    4天津市尖峰天然产物研究开发有限公司,天津 300000
  • 收稿日期:2011-08-30 修回日期:2011-09-07 出版日期:2011-09-26 发布日期:2011-10-11
  • 通讯作者: 吴勇杰,男,教授,博士研究生导师,研究方向:心血管药理及新药的研发。Tel: 13893445805 E-mail: wuyj@lzu.edu.cn
  • 作者简介:王艳红,女,硕士研究生,药师,研究方向:抗炎与免疫药理学。Tel: 13519319884 E-mail: wyh_0521@126.com
  • 基金资助:
    甘肃省自然科学基金资助项目(3ZS061-A25-083);国家自然基金资助项目(30672490)

Effect of proanthocyanidins from grape seeds on c-Jun and c-Fos expression in colon tissues of recurrent colitis rats

WANG Yan-hong1,2, DING Yi-lan3, YANG Xiao-lai2, GEI Bin2, LU Li1, ZHANG Bao-lai1, YU Hong-jian4, LIU Dan4, WU Yong-jie1   

  1. 1Department of Pharmacology, Lanzhou University, Lanzhou 730000, Gansu, China;
    2People's Hospital of Gansu Province, Lanzhou 730000, Gansu, China;
    3Lanzhou Women and Children's Hospital and Health Institute, Lanzhou 730030, Gansu, China;
    4Tianjin Jianfeng Natural Products Company, Tianjin 300000, China
  • Received:2011-08-30 Revised:2011-09-07 Online:2011-09-26 Published:2011-10-11

摘要: 目的: 观察葡萄籽原花青素(GSPE)对复发性结肠炎大鼠结肠组织中c-Jun、c-Fos表达的影响,探讨GSPE治疗复发性结肠炎的分子作用机制。方法: 雄性Wistar大鼠按体重随机分为正常对照组、模型对照组、柳氮磺吡啶(SASP)组和GSPE低(100 mg/kg)、中(200 mg/kg)、高(400 mg/kg)剂量组。用TNBS-50%乙醇二次致炎法建立大鼠溃疡性结肠炎(UC)复发的模型,于第2次致炎 24 h 后灌胃对其进行治疗,连续给药 7 d 后处死大鼠。取结肠标本进行大体与组织病理学损伤评分;用邻联茴香胺法检测结肠组织中髓过氧化物酶(MPO)活力以考察中性粒细胞浸润程度;应用酶联免疫法检测大鼠结肠组织中c-Jun与c-Fos的表达。结果: 与正常对照组比较,模型对照组大鼠大体和组织病理学损伤评分及组织中MPO活力显著增加,结肠组织中c-Jun与c-Fos表达明显升高。给予不同剂量的GSPE治疗7 d后,明显减轻复发性结肠炎大鼠大体和组织病理学损伤评分,减轻肠黏膜中性粒细胞的浸润程度,抑制结肠组织c-Jun与c-Fos的高表达。而柳氮磺吡啶组大鼠肠组织中c-Jun与c-Fos含量与模型对照组比较无明显差异(P>0.05)。结论: GSPE可以抑制复发性结肠炎大鼠结肠组织中c-Jun与c-Fos蛋白的高表达。

关键词: 葡萄籽原花青素, 三硝基苯磺酸(TNBS), 结肠炎, 复发, c-Jun, c-Fos

Abstract: AIM: To investigate the effect of proanthocyanidins from grape seeds (GSPE) on c-Jun and c-Fos expression in colon tissues of recurrent colitis rats. METHODS: Wistar male rats were randomized divided into six groups: normal control group, model control group, Sulfasalazine (SASP) group, and the 3 GSPE treatment groups. Rats were intragastrically administered different doses of GSPE (100, 200, and 400 mg/kg) per day for 7 days after recurrent colitis was twice-induced by TNBS-50% ethanol. Rats were killed after GSPE treatment 7 days. The colonic injury and inflammation were assessed by macroscopic and microscopic damage scores and myeloperoxidase (MPO) activity. The expression levels of c-Jun and c-Fos in the colon tissues were measured by enzyme-linked immunosorbent assay methods. RESULTS: Compared with the model group, the macroscopic and microscopic damage scores and the activity of MPO were significantly decreased in GSPE dosage group. Moreover, the expression levels of c-Jun and c-Fos in the colon tissues were significantly decreased. CONCLUSION: GSPE can inhibit the expression of c-Jun and c-Fos in the colon tissues of the recurrent colitis rats.

Key words: Proanthocyanidins from grape seeds, 2,4,6-trinitrobenzenesulfonic acid, Colitis, Re- current, c-Jun, c-Fos

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