淫羊藿苷对快速老化小鼠SAMP10脑组织单胺类及氨基酸类神经递质的影响

中国临床药理学与治疗学 ›› 2012, Vol. 17 ›› Issue (10): 1081-1086.

• 基础研究 • 下一篇

淫羊藿苷对快速老化小鼠SAMP10脑组织单胺类及氨基酸类神经递质的影响

高琳娜¹, 贺晓丽², 唐千淇³, 何国荣⁴, 毕明刚²

¹河北北方学院,张家口 075000,河北;
²中国医学科学院药用植物研究所,北京 100193;
³哈尔滨商业大学,哈尔滨 150076,黑龙江;
⁴中国医学科学院药物研究所,北京 100050

收稿日期:2012-03-13 修回日期:2012-05-03 发布日期:2012-10-19
通讯作者: 毕明刚,通信作者,男,博士, 研究员, 主要从事中药药理和免疫药理的研究。Tel: 010-57833504 E-mail: biminggang@yahoo.com.cn
作者简介:高琳娜,女,在读硕士研究生,主要从事神经药理的研究。Tel: 15313255828 E-mail: gao8167593@163.com
基金资助:
国家自然科学基金项目 (30772750); “重大新药创制”科技重大专项 (2009ZX09301-003)

Effects of icariin on monoamine and amino acid neurotransmitters in senescence accelerated mouse prone/10

GAO Lin-na¹, HE Xiao-li², TANG Qian-qi³, HE Guo-rong⁴, BI Ming-gang²

¹Hebei North University, Zhangjiakou 075000,Hebei,China;
²Institute of Materia Medica,Peking Union Medical College&Chinese Academy of Medical Sciences, Beijing 100193,China;
³Harbin University of Commerc, harbin 150076,Heilongjiang,China;
⁴Institute of Materia Medica, Chinese Academy of Medical Sciences, Beijing 100050,China

Received:2012-03-13 Revised:2012-05-03 Published:2012-10-19

摘要/Abstract

摘要： 目的: 探讨淫羊藿苷(ICA)对快速老化小鼠SAMP10脑组织单胺类及氨基酸类神经递质的影响。方法: 本实验采取8月龄快速老化小鼠SAMP10为实验对象,随机分为模型SAMP10组、阳性药多奈哌齐组(1 mg/kg)、ICA 3个剂量(50、100、200 mg/kg)组,每组12只,以12只同月龄抗快速老化小鼠SAMR1为正常对照。灌胃给药 30 d,一天一次,高效液相-电化学法检测快速老化小鼠SAMP10大脑皮层的去甲肾上腺素(NE)、5-羟色胺(5-HT)、多巴胺(DA)及其代谢产物3,4-二羟苯乙酸(DOPAC)和高香草酸(HVA)的含量来探讨ICA对SAMP10脑组织单胺类神经递质的影响,通过检测谷氨酸(Glu)、谷氨酰胺(Gln)、天冬氨酸(Asp)、γ-氨基丁酸(GABA)、牛磺酸(Tau)、甘氨酸(Gly)的含量来探讨ICA对SAMP10脑组织氨基酸类神经递质的影响。结果: ICA可显著降低SAMP10大脑皮层内Glu、Gln、GABA含量(P<0.01),升高NE、DA、DOPAC、5-HT、HVA、Asp以及Tau的含量(P<0.01 或P<0.05),但对SAMP10大脑皮层内Gly的含量并没有显著影响(P>0.05)。结论: ICA可能通过升高脑内单胺类神经递质的含量、调节兴奋性氨基酸类递质的代谢平衡以及调节抑制性氨基酸的代谢平衡达到改善SAMP10的学习记忆的作用。

关键词: 淫羊藿苷, 快速老化小鼠SAMP10, 单胺, 氨基酸

Abstract: AIM: To investigate the effects of icariin(ICA) on monoamine and amino acid neurotransmitters in senescence accelerated mouse prone/10(SAMP10). METHODS: The 8-month-old SAMP10 were randomly divided into model group, Donepezil Hydrochloride(DON)-treated SAMP10 group (1 mg/kg) and ICA-treated SAMP10 groups(50, 100, 200 mg/kg), with twelve mice in each group. Twelve senescence accelerated mouse/resistance1(SAMR1) with the same age were used for normal control. After 30 days of oral administration, HPLC with electrochemical detection (EC) was used to measure norepinephrine, 5-hydroxytryptamine, dopamine and its metabolites 3,4-phenyl acetic acid and homovanillic acid, and glutamic acid,glutamine,aspartic acid, γ-aminobutyric acid,taurine,glycine.RESULTS: ICA could significantly reduce the levels of glutamic acid, glutamine and γ-aminobutyric acid in the cerebral cortex of SAMP10 (P<0.01), and increase the levels of norepinephrine, dopamine, 3, 4 - phenyl acetic acid,5 - hydroxytryptamine, homovanillic acid, aspartic acid and taurine (P<0.01 or P<0.05), but glycine had no significant effect(P>0.05). CONCLUSION: The improvement of ICA on the ability of learning and memory in SAMP10 may be related to elevating levels of monoamine neurotransmitter, regulating the balance of excitatory amino acids and regulating the balance of inhibitory amino acids.

Key words: Icariin, Senescence accelerated mouse prone/10, Monoamine, Amino acid

中图分类号:

R965.2

高琳娜, 贺晓丽, 唐千淇, 何国荣, 毕明刚. 淫羊藿苷对快速老化小鼠SAMP10脑组织单胺类及氨基酸类神经递质的影响[J]. 中国临床药理学与治疗学, 2012, 17(10): 1081-1086.

GAO Lin-na, HE Xiao-li, TANG Qian-qi, HE Guo-rong, BI Ming-gang. Effects of icariin on monoamine and amino acid neurotransmitters in senescence accelerated mouse prone/10[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2012, 17(10): 1081-1086.

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淫羊藿苷对快速老化小鼠SAMP10脑组织单胺类及氨基酸类神经递质的影响

Effects of icariin on monoamine and amino acid neurotransmitters in senescence accelerated mouse prone/10

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