唐古特大黄多糖组分1对电离辐射损伤小鼠造血系统的影响

中国临床药理学与治疗学 ›› 2012, Vol. 17 ›› Issue (10): 1087-1090.

唐古特大黄多糖组分1对电离辐射损伤小鼠造血系统的影响

刘琳娜¹, 郭志伟², 张琰¹, 张甜¹, 张文娟¹, 戚志华¹, 徐媛¹

¹第四军医大学唐都医院药剂科,西安 710038,陕西;
²新疆布尔津县人民医院,布尔津 836600,新疆维吾尔自治区

收稿日期:2012-04-21 修回日期:2012-07-19 发布日期:2012-10-19
通讯作者: 刘琳娜,通信作者,女,博士,副主任药师,研究方向:中药药理与分子药理。Tel: 029-84777631 E-mail: liulinna@fmmu.edu.cn
基金资助:
国家自然科学基金资助项目(81073135)

Protective effects of purified fraction 1 of polysaccharide extracted from rheum tanguticum on radiation-induced hematopoietic damage in mice

LIU Lin-na¹, GUO Zhi-wei², ZHANG Yan¹, ZHANG Tian¹, ZHANG Wen-juan¹, Qi Zhi-hua¹, XU Yuan¹

¹Department of Pharmacy, Tangdu Hospital, Fourth Military Medical University, Xi'an 710038, Shaanxi, China;
²Buerjin County People's Hospital, Buerjin 836600, Xinjiang Uyghur Autonomous Region, China

Received:2012-04-21 Revised:2012-07-19 Published:2012-10-19

摘要/Abstract

摘要： 目的: 探讨唐古特大黄多糖组分1(RTP1)对电离辐射损伤小鼠造血功能的影响。方法: 采用雄性6周龄昆明种小鼠,随机分为5组:正常对照组(Normal Control,NC)、辐射对照组(Irradiation Control,IC)以及RTP1低剂量组(200 mg/kg)、中剂量组(400 mg/kg)和高剂量组(800 mg/kg),采用灌胃给药方式,连续 14 d,NC组和IC组则给予等量的生理盐水,第 14 d 除NC组外,各组小鼠均接受 6.5 Gy/只⁶⁰Coγ射线照射1次,照射后继续灌胃给药,观察照射后 30 d 小鼠生存情况,并对小鼠骨髓有核细胞数(bone marrow cell, BMC)、骨髓DNA含量、内源性脾集落形成单位(spleen colony forming unit, CFU-S)及外周血中白细胞(white blood cell, WBC)计数进行检测。结果: 与IC组比较,RTP1可提高小鼠 30 d 存活率,并剂量依赖性地升高外周血中WBC计数、骨髓有核细胞数、DNA含量以及脾结节数。结论: RTP1对辐射小鼠的造血系统起到一定保护作用。

关键词: 唐古特大黄多糖, 辐射损伤, ⁶⁰Coγ射线;, 造血系统

Abstract: AIM: To study the protective effects of purified fraction 1 of polysaccharide extracted from rheum tanguticum (RTP1) on radiation-induced hematopoietic damage in mice. METHODS: Male Kunming mice which were 6 week old were randomly divided into five groups: normal group (NC), irradiation control group (IC), RTP1 low dose group (200 mg/kg), middle dose group (400 mg/kg) and high dose group (800 mg/kg).The mice in RTP1 group were poured through gastic route by RTP1 for 14 d, the mice in NC and IC group were given by 0.9% sodium chloride solution through the same way. The mice in all groups except NC group were irradiated with 6.5 Gy ⁶⁰Co γ-ray on the fourteenth day. The 30 d survival rate of mice was observed. The number of white blood cell (WBC) and bone marrow cell (BMC), as well as the count of endo-genous splenic colony-forming-unit (CFU-S) were detected. DNA content of marrow was also measured.RESULTS: Compared with that of the IC group, 30 d survival rate, the number of WBC and BMC, the count of CFU-S and the DNA content of marrow were significantly higher with a dose- dependent manner. CONCLUSION: RTP1 could improve the hematopoietic recovery after irradiation.

Key words: Polysaccharide of rheum tanguticum, radiation damage, ⁶⁰Coγ ray;, Hematopoietic system

中图分类号:

R147
R965.2

刘琳娜, 郭志伟, 张琰, 张甜, 张文娟, 戚志华, 徐媛. 唐古特大黄多糖组分1对电离辐射损伤小鼠造血系统的影响[J]. 中国临床药理学与治疗学, 2012, 17(10): 1087-1090.

LIU Lin-na, GUO Zhi-wei, ZHANG Yan, ZHANG Tian, ZHANG Wen-juan, Qi Zhi-hua, XU Yuan. Protective effects of purified fraction 1 of polysaccharide extracted from rheum tanguticum on radiation-induced hematopoietic damage in mice[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2012, 17(10): 1087-1090.

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