雷莫司琼降低幼鼠内脏痛觉敏感性

中国临床药理学与治疗学 ›› 2012, Vol. 17 ›› Issue (10): 1102-1107.

雷莫司琼降低幼鼠内脏痛觉敏感性

林希¹, 杨帆¹, 吴斌¹, 张晓燕¹, 林春²

¹福建医科大学附属第一医院儿科,福州 350004,福建;
²福建医科大学生理与病生学系,福州 350004,福建

收稿日期:2012-04-02 修回日期:2012-07-12 发布日期:2012-10-19
作者简介:林希,女,主治医师,研究方向:小儿神经系统疾病。E-mail: linxicc@yahoo.com.cn
基金资助:
福建医科大学苗圃科研基金项目(2010MP012)

Ramosetron decrease visceral pain hypersensitivity in young rats

LIN Xi, YANG Fang, WU Bin, ZHANG Xiaoyan, LIN Chun

Department of Pediatrics, the First Affiliated Hospital of Fujian Medical University, Fuzhou 350004, Fujian, China

Received:2012-04-02 Revised:2012-07-12 Published:2012-10-19

摘要/Abstract

摘要： 目的: 采用行为学和电生理学评价方法,探讨应用5-羟色胺3(5-HT₃)受体拮抗剂雷莫司琼对幼鼠内脏痛觉敏感性影响。方法: 新生期反复结直肠刺激(colorectal irritation,CI)建立幼鼠内脏痛觉高敏感性模型。32只SD新生大鼠按2×2析因设计分成4组,每组8只,A₁B₁组:新生期反复CI,15~21 d 腹腔注射雷莫司琼;A₁B₂组:新生期反复CI,15~21d 腹腔注射生理盐水;A₂B₁组:新生期未接受CI,15~21 d 腹腔注射雷莫司琼;A₂B₂组:新生期未接受CI,15~21 d 腹腔注射生理盐水。常规饲养到幼鼠期(6周龄),通过观察幼鼠在不同压力结直肠扩张(colorectal distension,CRD)刺激后的腹壁撤退反射(AWR)评分、内脏痛阈和腹外斜肌放电测量进行内脏痛觉敏感性评价。结果: 幼鼠痛阈受建立内脏痛敏化模型和早期雷莫司琼干预两因素影响。建立内脏痛敏化模型可使幼鼠痛阈降低 11.56 mm Hg,早期雷莫司琼干预则使幼鼠痛阈提高 9.06 mm Hg,两者存在交互作用。应用雷莫司琼使AWR评分及腹外斜肌放电幅值降低,对于AWR评分的主效应在各个CRD压力下有统计学意义;对腹外斜肌放电幅值的主效应在各不同CRD压力下亦有统计学意义。结论:应用5?HT3受体拮抗剂雷莫司琼能够降低幼鼠内脏痛觉的高敏感性。

关键词: 内脏痛高敏感, 5-羟色胺3受体拮抗剂, 雷莫司琼, 幼鼠

Abstract: AIM: To explore the effects of 5-HT3 receptor antagonist ramosetron on young rats of visceral hypersensitivity by the methods of behavior and electrophysiology research. METHODS: Colorectal irritation (CI) stimulation in neonatal period was used to establish the model of visceral hypersensitivity in young rats. 32 neonatal rats were divided into four groups by 2×2 factorial design with each 8. Group A₁B₁ was imposed on CI at neonatal period and imposed on ramosetron intraperitoneal injection in 15-21 day; Group A₁B₂ was imposed on CI at neonatal period and not imposed on ramosetron intraperitoneal injection; Group A₂B₁ was not imposed on CI at neonatal period and imposed on ramosetron intraperitoneal injection; Group A₂B₂ was not imposed on CI at neonatal period and not imposed on ramosetron intraperitoneal injection. Then, conventionally breeding till the young period (6-week age), abdonminal withdrawal reflex (AWR), pain thresholds and spike external oblique muscle of abdomen (EOMA) were used to evaluate visceral pain hypersensitivity. RESULTS: he pain thresholds in young rats were affected both by CI at neonatal period and using ramosetron in 15-21 days. In group A₁B₂ which imposed on CI at neonatal period, the pain thresholds decreased 11.56 mm Hg. In group A₁B₁ which imposed on CI at neonatal period and imposed on ramosetron in 15-21 day, the pain thresholds increased 9.06 mm Hg, while the AWR scores and EOMA were decreased. Future more, there are interaction effects between the two factors.There were a significant main effect for AWR scores at different pressures and a significant main effect for EOMA at different pressures. CONCLUSION: 5-HT₃ receptor antagonist ramosetron can decrease visceral hypersensitivity in young rats.

Key words: Visceral pain hypersensitivity, 5-HT₃ receptor antagonist, Ramosetron, Young rats

中图分类号:

R965.2

林希, 杨帆, 吴斌, 张晓燕, 林春. 雷莫司琼降低幼鼠内脏痛觉敏感性[J]. 中国临床药理学与治疗学, 2012, 17(10): 1102-1107.

LIN Xi, YANG Fang, WU Bin, ZHANG Xiaoyan, LIN Chun. Ramosetron decrease visceral pain hypersensitivity in young rats[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2012, 17(10): 1102-1107.

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