明胶海绵复合重组人骨形态发生蛋白-7植入物的体内异位成骨实验

中国临床药理学与治疗学 ›› 2012, Vol. 17 ›› Issue (12): 1349-1354.

明胶海绵复合重组人骨形态发生蛋白-7植入物的体内异位成骨实验

王国栋¹, 李萍¹, 韩金祥², 王世立², 刘丽娜³

¹皖南医学院药学系,芜湖 241002,安徽;
²山东省医药生物技术研究中心国家卫生部生物技术药物重点实验室,济南 250062,山东;
³皖南医学院弋矶山医院,芜湖 241001,安徽

收稿日期:2012-06-07 修回日期:2012-08-22 发布日期:2012-12-31
通讯作者: 王世立,通信作者,男,研究员,硕士生导师,研究方向:生化药理学。Tel: 0531-82919608 E-mail: shili2005@sina.com
作者简介:王国栋,男,硕士,讲师,研究方向:生物技术药物。Tel: 0553-3932414 　E-mail: guodong201@163.com
基金资助:
国家高技术研究发展计划(863)项目(2003AA2Z3532); 安徽省高校省级自然科学研究项目(KJ2009B154); 皖南医学院校中青年科研基金项目(WK201203)

Experimental study on ectopic osteogenesis by implants of gelatin sponge combined with recombinant human bone morphogenetic protein-7

WANG Guo-dong¹, LI Ping¹, HAN Jin-xiang², WANG Shi-li², LIU Li-na³

¹Department of Pharmacy, Wannan Medical College, Wuhu 241002, Anhui, China;
²Shandong Medicinal Biotechnology Center, the Key Laboratory for Biotech-Drugs of Ministry of Health, Jinan 250062, Shandong, China;
³Yijishan Hospital of Wannan Medical College, Wuhu 241001, Anhui, China

Received:2012-06-07 Revised:2012-08-22 Published:2012-12-31

摘要/Abstract

摘要： 目的: 考察以明胶海绵为载体的重组人骨形态发生蛋白-7(rhBMP-7)在小鼠体内异位成骨能力,评价大肠杆菌表达的rhBMP-7生物学活性。方法: 昆明种小鼠随机分为两组,将复合rhBMP-7的明胶海绵植入小鼠肌间隙作为实验组,对照组植入明胶海绵,分别于术后1、 2、3、4周取出埋植材料,HE染色进行组织学观察,测定蛋白含量、钙含量与碱性磷酸酶(ALP)活性。结果: 复合rhBMP-7的明胶海绵组在术后2、3、4周有骨细胞类似细胞和钙化灶的形成,术后2、3、4周蛋白含量、ALP活性和钙含量均明显高于明胶海绵对照组。结论: 明胶海绵复合 rhBMP-7植入小鼠体内有较强的异位成骨能力,是良好的生物载体,可用于 rhBMP-7 体内活性检测。

关键词: 明胶海绵, 重组人骨形态发生蛋白-7, 异位成骨, 小鼠

Abstract: AIM: To investigate the ectopic osteogenetic capacity of gelatin sponge contained with recombinant human bone morphogenetic protein-7 (rhBMP-7) by animal experiment and evaluate the biologic activity of rhBMP-7 expressed in Escherichia coli. METHODS: KM mice were divided into two groups randomly. Gelatin sponge contained with rhBMP-7 were implanted into intramuscular spatium of mice as experimental group, while gelatin sponge were implanted as control group. The mice were sacrificed and the tissue samples were harvested at 1st, 2nd, 3rd and 4th week after implantation. Tissue response was observed by HE staining. The contents of proteins and calcium were measured and the activities of alkaline phosphatase (ALP) were detected. RESULTS: HE staining showed that some cells similar to osteocytes and calcific tissues were formed in the group of gelatin sponge contained with rhBMP-7 after implantation at 2nd, 3rd and 4th week. The contents of proteins and calcium and the activities of ALP in experimental group were significantly higher than those in the control group which implanted with gelatin sponge at 2nd, 3rd and 4th week.CONCLUSION: The gelatin sponge combined with rhBMP-7 could induce ectopic osteogenesis more effectively and it is a good biomaterial which could be used to detect the biological activity of rhBMP-7.

Key words: Gelatin sponge, Recombinant human bone morphogenetic protein-7, Ectopic osteogenesis, Mice

中图分类号:

王国栋, 李萍, 韩金祥, 王世立, 刘丽娜. 明胶海绵复合重组人骨形态发生蛋白-7植入物的体内异位成骨实验[J]. 中国临床药理学与治疗学, 2012, 17(12): 1349-1354.

WANG Guo-dong, LI Ping, HAN Jin-xiang, WANG Shi-li, LIU Li-na. Experimental study on ectopic osteogenesis by implants of gelatin sponge combined with recombinant human bone morphogenetic protein-7[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2012, 17(12): 1349-1354.

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