甘露醇对大鼠离体心脏心功能的影响

中国临床药理学与治疗学 ›› 2012, Vol. 17 ›› Issue (12): 1368-1372.

甘露醇对大鼠离体心脏心功能的影响

陈敏¹, 张明升¹, 王燕¹, 梁月琴²

¹山西医科大学药理学教研室, ²山西医科大学机能实验室, 太原 030001, 山西

收稿日期:2012-07-24 修回日期:2012-11-06 出版日期:2012-12-26 发布日期:2012-12-31
通讯作者: 张明升,通信作者,男,教授,博士生导师,研究方向:心血管药理。
作者简介:陈敏,女,博士,讲师,研究方向:心血管药理。Tel: 13453198013 E-mail: wscm2003screen@yahoo.com.cn
基金资助:
山西省自然科学基金项目[(2012)2012021033-5]

Effects of mannitol on the function in isolated hearts of rats

CHEN Min¹, ZHANG Ming-sheng¹, WANG Yan¹, LIANG Yue-qin²

¹Department of Pharmacology, ²Functional Laboratory, Shanxi Medical University, Taiyuan 030001, Shanxi, China

Received:2012-07-24 Revised:2012-11-06 Online:2012-12-26 Published:2012-12-31

摘要/Abstract

摘要： 目的: 研究甘露醇对离体大鼠心脏心功能的影响。方法: 应用Langendorff大鼠离体心脏灌流方法。32只雄性SD大鼠,随机分为4组:对照组,甘露醇 20 mmol/L 组,甘露醇 40 mmol/L 组,甘露醇 60 mmol/L 组,每组8只。连续记录用药后 60 min 冠状动脉流量、心脏收缩期左室主动收缩压(left ventricular developed pressure,LVDP)、左心室内压上升的最大变化速率(+dp/dt_max)、心率与左室主动收缩压的乘积(RPP)、舒张期左心室内压下降的最大变化速率(-dp/dt_max)及左心室舒张末压(left ventricular end-diastolic pressure,LVEDP)并进行统计分析。结果: 与用药前相比,甘露醇 20 mmol/L 组随着作用时间延长,可逐渐升高LVDP、+dp/dt_max、RPP,60 min 时达最高。甘露醇 40 mmol/L 升高LVDP,+dp/dt_max、RPP,30 min 左右达高峰后逐渐降低。甘露醇60 mmol/L升高LVDP,+dp/dt_max、RPP,10 min 左右达高峰,随后衰减。高浓度甘露醇升高LVEDP,降低-dp/dt_max。结论: 不同浓度甘露醇对左心室收缩功能的影响具有时间依赖性。高浓度甘露醇减弱左心室舒张功能。

关键词: 甘露醇, 离体心脏, 灌流

Abstract: AIM: To observe the effects of mannitol in isolated rat hearts. METHODS: The langendorff system was used to evaluate the function of rat hearts treated with mannitol (20 mmol/L, 40 mmol/L and 60 mmol/L respectively). Following a stabilization period of 30 min, the isolated hearts were subjected to continuous perfusion for a further 60 min with vehicle or increasing concentration of mannitol. Heart functional indexes including left ventricular develop pressure (LVDP), rate-pressure product (RPP), maximal rate of pressure increased (+dpp/dt_max), maximal rate of pressure decreased (-dp/dt_max), left ventricular end diastolic pressure (LVEDP) and coronary flow were recorded.RESULTS: LVDP,+dp/dt_max and RPP were increased significantly in hearts perfused with 20 mmol/L mannitol in a time dependent manner. However, when the hearts perfused with 40 mmol/L or 60 mmol/L mannitol, time used for reaching the maximum LVDP,+dp/dt_max and RPP by 40 mmol/L and 60 mmol/L mannitol was shorter in hearts treated with 20 mmol/L mannitol. More, the results demonstrated that heart treated with 60 mmol/L mannitol could significantly increase the LVDEP and decrease the -dp/dt_max. CONCLUSION: Mannitol could affect left ventricular contraction function in a time and concentration dependent manner. Ventricular diastolic function was weakened by perfusing the high concentration of mannitol.

Key words: Mannitol, Isolated heart, Perfusion

中图分类号:

R965.2

陈敏, 张明升, 王燕, 梁月琴. 甘露醇对大鼠离体心脏心功能的影响[J]. 中国临床药理学与治疗学, 2012, 17(12): 1368-1372.

CHEN Min, ZHANG Ming-sheng, WANG Yan, LIANG Yue-qin. Effects of mannitol on the function in isolated hearts of rats[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2012, 17(12): 1368-1372.

参考文献

[1] Paterson IS, Klausner JM, Goldman G, et al.Pulmonary edema after aneurysm surgery is modified by mannitol[J]. Ann Surg, 1989, 210(6): 796-801.
[2] Toung TJK, Chang Y, Lin J, et al.Increase in lung and brain water following experimental stroke: effect of mannitol and hypertonic saline[J]. Crit Care Med, 2005, 33(1): 203-208.
[3] 刘树峰, 尹兵. 甘露醇在心脑血管疾病的临床应用[J]. 医药导报, 1998, 17(3): 190-191.
[4] 陶静,胡杰,高琴,等. 大豆异黄酮预防离体大鼠心肌缺血再灌注损伤[J]. 中国临床药理学与治疗学, 2011, 16(10):1090-1095.
[5] 李俊峡,王风岚,郭加旺,等. 甘露醇治疗冠心病顽固性心绞痛临床观察[J]. 临床荟萃,1997, 12(2):81-82.
[6] 弓玉冰,张玉梅. 甘露醇治疗顽固性心绞痛16例报告[J]. 临床荟萃,1995,10(11): 503.
[7] Krishnamurty VS, Ross G.Influence of hyperosmolarity on induced contractions of arterial smooth muscle in vitro[J]. Arch Int Pharmacodyn Ther, 1984, 270(1): 38-49.
[8] Krishnamurty VS, Adams HR, Smitherman TC, et al.Influence of mannitol on contractile responses of isolated perfused arteries[J]. Am J Physiol, 1977, 232(1): H59-66.
[9] Toda N, Ayajiki K, Toda H, et al.Mechanism underlying mannitol-induced relaxation in isolated monkey cerebral arteries[J]. Am J Physiol, 1992, 262(3): H897-H902.
[10] Crystal GJ, Gurevicius J, Kim SJ, et al.Effects of hypertonic saline solutions in the coronary circulation[J]. Circ Shock, 1994, 42(1): 27-38.
[11] Li J, Wang MH, Wang L, et al.Role of transient receptor potential vanilloid 4 in the effect of osmoticpressure on myocardial contractility in rat[J]. Sheng Li Xue Bao, 2008, 60(2): 181-188.
[12] Willerson JT, Powell WJ, Guiney TE, et al.Improvement in myocardial function and coronary blood flow in ischemic myocardium after mannitol[J]. J Clin Invest, 1972, 51(12): 2989-2998.
[13] Fixler DE, Buja LM, Wheeler JM, et al.Influence of mannitol on maintaining coronary flows and salvaging myocardium during ventriculotomy and during prolonged coronary artery ligation[J]. Circulation, 1977, 56(3): 340-346.
[14] Willerson JT, Watson JT, Platt MR.Effect of hypertonic mannitol and intraaortic counterpulsation on regional myocardial blood flow and ventricular performance in dogs during myocardial ischemia[J]. Am J Cardiol, 1976, 37(4): 514-519.