γ-氨基丁酸在围植入期小鼠子宫的含量及其意义

中国临床药理学与治疗学 ›› 2012, Vol. 17 ›› Issue (2): 154-158.

γ-氨基丁酸在围植入期小鼠子宫的含量及其意义

罗文萍, 赵海, 刘真真, 谭冬梅, 谭毅

重庆医科大学实验动物中心,重庆 400016

收稿日期:2011-12-30 修回日期:2012-02-15 出版日期:2012-02-26 发布日期:2012-03-12
通讯作者: 谭毅,男,博士,研究员,主要从事胚胎植入与肿瘤侵袭转移的比较研究。Tel: 023-68486187 E-mail: tanyee66@hotmail.com
作者简介:罗文萍,女,博士研究生,主要从事胚胎植入机理的基础研究。E-mail: lwplucky@163.com
基金资助:
国家自然科学基金面上项目 (30270510;31171436)

γ-Amino butyric Acid in peri-implantation mouse uterine contents and its significance

LUO Wen-ping, ZHAO Hai, LIU Zhen-zhen, TAN Dong-mei, TAN Yi

Laboratory Animal Center, Chongqing Medical University, Chongqing 400016,China

Received:2011-12-30 Revised:2012-02-15 Online:2012-02-26 Published:2012-03-12

摘要/Abstract

摘要： 目的: 通过检测γ-氨基丁酸(GABA)在围植入期第1日到第8日小鼠子宫的含量;并腹腔注射GABA,探讨其在围植入期小鼠子宫中的含量变化及对子宫蜕膜化的影响。方法: 比色法检测妊娠小鼠第1日到第8日子宫中GABA含量;并于妊娠第5日开始腹腔注射GABA,浓度分别为 0.05、0.5、5 g/kg,每日1次,至第8日时称重蜕膜鼓包及石蜡切片后行HE染色,测量蜕膜化区域面积。结果: 与第1日比较,第3日中GABA含量上调(P＜0.05),第4日时出现下降趋势,却在第5日植入位点处出现最大值(P＜0.01),且第6到8日的非植入位点处GABA含量显著高于植入位点;体内实验发现,0.05 g/kg GABA及 5 g/kg GABA可显著减少蜕膜鼓包数量,0.5 g/kg GABA可显著降低蜕膜鼓包重量。同时,0.05 g/kg GABA 及 0.5 g/kg GABA可显著减少蜕膜化区域面积。结论: γ-氨基丁酸含量在围植入期小鼠子宫中呈现动态变化,并可能参与抑制妊娠小鼠子宫蜕膜化。

关键词: γ-氨基丁酸, 比色法, 蜕膜

Abstract: AIM: To identify the content of GABA in peri-implantation mouse uterine and its effects on decidualization by intraperitoneal injection different concentration of GABA.METHODS: The contents of GABA in peri-implantation mouse uterine from day 1 to day 8 were measured by chromatometry. From day 5, 0.05 g/kg,0.5 g/kg,5 g/kg concentrations of GABA were injected by abdominal cavity until day 8. Decidual tissues were weighted and then made paraffin section followed by HE staining. Areas of decidual were measured by Image J software.RESULTS: The contents of GABA in the pregnancy of D3 utrerine upregulated followed decreased in D4 utrerine but the highest content of GABA was occurred in D5 implantion sites uterine compared with that of in D1 utrerine. The content of GABA from D6 to D8 utrerine in iner- implantion sites were higher than implantion sites. Both 0.05 g/kg GABA and 5 g/kg GABA could reduced the number of deciduum, while both 0.5 g/kg GABA could down-regulated the weight of deciduum. Both 0.05 g/kg GABA and 0.5 g/kg GABA could reduce the area of decidual region.CONCLUSION: The contents of GABA showed dynamic change in peri-implantation mouse uterine which may be invloved in inhibited decidualization.

Key words: γ-Amino butyric Acid, Chromatometry, Decidualization

中图分类号:

Q492
R915

罗文萍, 赵海, 刘真真, 谭冬梅, 谭毅. γ-氨基丁酸在围植入期小鼠子宫的含量及其意义[J]. 中国临床药理学与治疗学, 2012, 17(2): 154-158.

LUO Wen-ping, ZHAO Hai, LIU Zhen-zhen, TAN Dong-mei, TAN Yi. γ-Amino butyric Acid in peri-implantation mouse uterine contents and its significance[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2012, 17(2): 154-158.

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