热休克蛋白70肽段抑制胶原诱导关节炎小鼠Th17细胞生成

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中国临床药理学与治疗学 ›› 2012, Vol. 17 ›› Issue (4): 392-397.

• 基础研究 • 上一篇下一篇

热休克蛋白70肽段抑制胶原诱导关节炎小鼠Th17细胞生成

郭鸿雁^1,2, 袁慧慧¹, 张须龙¹, 赵文明¹

¹首都医科大学基础医学院免疫学系,北京100069;
²首都医科大学附属北京佑安医院临检中心,北京 100069

收稿日期:2012-01-05 修回日期:2012-03-14 发布日期:2012-04-28
通讯作者: 赵文明,男,教授,博士生导师,研究方向:自身免疫病。Tel: 010-83911824 E-mail: zhao-wenming@163.com
作者简介:郭鸿雁,男,硕士研究生,研究方向:自身免疫病。Tel: 13693035095 E-mail: ghy0629@163.com
基金资助:
国家自然科学基金(30873185);973计划项目(2010CB529106)资助

Heat shock protein 70 peptide inhibits Th17 cells in collagen-induced arthritis mice

GUO Hong-yan^1,2, YUAN Hui-hui¹, ZHANG Xu-long¹, ZHAO Wen-ming¹

¹Department of Immunology, Capital Medical University, Beijing 100069, China;
²Clinical Laboratory of Beijing You'an Hospital, Capital Medical University, Beijing 100069, China

Received:2012-01-05 Revised:2012-03-14 Published:2012-04-28

摘要/Abstract

摘要： 目的: 研究HSP70肽段对CIA小鼠Th17细胞的影响,探讨其抑制CIA小鼠炎症损伤的机制。方法: 将DBA/1小鼠随机分为HSP70肽段实验组、阴性对照组、结核菌素(PPD)对照组、阳性对照组。除阴性对照组外,其它各组小鼠用牛Ⅱ型胶原蛋白诱导关节炎。通过关节评分和病理改变评价病变程度。取外周淋巴结用流式细胞仪检测Th17细胞的数量,用实时定量PCR检测Th17细胞相关调控因子。结果: 注射HSP70肽段的CIA小鼠其Th17细胞数量明显低于阳性对照组和PPD对照组,促进Th17细胞分化发育的相关因子IL-6、IL-23、TGF-β₁ 、RORγt的表达水平低于阳性对照组和PPD对照组(P<0.05)。结论: HSP70肽段可调节Th17细胞分化的调控因子,抑制Th17细胞的分化,进而减轻CIA小鼠的炎症程度。

关键词: HSP70肽段, Th17细胞, CIA小鼠

Abstract: AIM: The effect of heat shock protein 70 (HSP70) peptide on Th17 cells was studied in collagen-induced arthritis(CIA) mice model and the mechanism of its inhibitory effect on inflammation was also investigated. METHODS: DBA/1 mice were randomly divided into four groups including HSP70 peptide group, negative control group, PPD treated group and positive control group. Arthritis was induced by immunizing bovine typeⅡcollage, except negative control group. Pathology was evaluated by paw swelling and histological changes. The number of Th17 cells in peripheral lymph nodes was assayed by flow cytometry, and Th17 cell-associated regulatory factors were measured by real-time quantitative PCR. RESULTS: The number of Th17 cells in HSP70 peptide treated group was significantly lower than that of positive control group and PPD treated group. Expression levels of IL-6, IL-23, TGF-β₁ and orphan nuclear receptor(RORγt) in HSP70 peptide group were markedly reduced than those of positive group and PPD group. CONCLUSION: HSP70 peptide could play a protective role on CIA mice through decreasing Th17 cell regulating factors and inhibiting the differentiation of Th17 cells.

Key words: Heat shock protein 70, T helper 17 cells, Collagen-induced arthritis mice

中图分类号:

R392

郭鸿雁, 袁慧慧, 张须龙, 赵文明. 热休克蛋白70肽段抑制胶原诱导关节炎小鼠Th17细胞生成[J]. 中国临床药理学与治疗学, 2012, 17(4): 392-397.

GUO Hong-yan^1,2, YUAN Hui-hui¹, ZHANG Xu-long¹, ZHAO Wen-ming¹. Heat shock protein 70 peptide inhibits Th17 cells in collagen-induced arthritis mice[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2012, 17(4): 392-397.

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