异丙酚预处理对缺氧/复氧后培养海马神经元金属硫蛋白-3蛋白表达的影响

中国临床药理学与治疗学 ›› 2012, Vol. 17 ›› Issue (5): 517-520.

异丙酚预处理对缺氧/复氧后培养海马神经元金属硫蛋白-3蛋白表达的影响

何建国, 黄长顺, 姜娟, 胡烨, 曹刚, 刘振伟

宁波市第一医院麻醉科,宁波 315010,浙江

收稿日期:2012-02-13 修回日期:2012-04-25 出版日期:2012-05-26 发布日期:2012-05-28
通讯作者: 黄长顺,男,本科,主任医师,研究方向:血液保护与脑保护。Tel: 13957882779 E-mail: nbhcs@yahoo.com.cn
作者简介:何建国,男,硕士,主治医生,研究方向:麻醉药与脑保护。Tel: 13567845064 E-mail: gyhjg2005@126.com

The impact of propofol pretreatment on hypoxia/reoxygenation in cultured hippocampal neurons metallothionein-3 protein expression

HE Jian-guo, HUANG Chang-shun, JIANG Juan, HU Ye, CAO Gang, LIU Zhen-wei

Department of Anesthesiology, Ningbo First Hospital, Ningbo 315000, Zhejiang, China

Received:2012-02-13 Revised:2012-04-25 Online:2012-05-26 Published:2012-05-28

摘要/Abstract

摘要： 目的:本研究观察异丙酚对培养海马神经元缺氧复氧后损伤反应的影响及金属硫蛋白-3(metallothionein-3,MT-3)在其中的作用。方法: 培养7~10 d 海马神经元,以不同浓度的异丙酚(50,150,250 μmol/L)预处理后 24 h 后,制备缺氧 4 h 后复氧 24 h 模型(H/R),Western blot法检测MT-3蛋白表达水平;以MT-3特异性siRNA沉默MT-3表达后,采用MTT法观察异丙酚对H/R海马神经元存活率的影响。结果: 在H/R条件下,异丙酚可浓度依赖性促进MT-3的蛋白表达。以MT-3 siRNA抑制MT-3蛋白表达后,异丙酚提高神经元存活率的作用消失。结论:异丙酚具有神经元保护效应,这一作用可能与其上调MT-3的蛋白表达有关。

关键词: 异丙酚, 金属硫蛋白-3, 海马神经元, 缺氧/复氧

Abstract: AIM: To observe the effects of propofol on caltured hippocampal neurons hypoxia and reoxygenation damage response and metallothionein protein-3(metllothionein-3,MT-3) in which the role.METHODS: Hippocampal neuron cells were cultured for 7-10 days for use. Hypoxic conditions were attained 4 h and reoxygenation was achieved with normoxia conditions for another 24 h. The expression level of metallothionein-3 (MT-3) was detected by Western blot; By using MT-3 specific siRNA, MT-3 was silenced and the effect of propofol on cell survival rate was evaluated by MTT method .RESULTS:In cultured hippocampal neuron cells, pretreatment with different concentrations of propofol (50, 150, 250 mol/L) for 24 h enhanced MT-3 expression level after hypoxia/reoxygenation. When MT-3 expression was silenced by specific siRNA, propofol failed to improve hippocampal neuron cell survival rate.CONCLUSION: MT-3 exerts hippocampal neuron protective effect, which may possibly be mediated by the upreguation of MT-3 expression.

Key words: Propofol, Metallothionein-3, Hippocampal neuron cells, Hypoxia/reoxygenation

中图分类号:

R965.2

何建国, 黄长顺, 姜娟, 胡烨, 曹刚, 刘振伟. 异丙酚预处理对缺氧/复氧后培养海马神经元金属硫蛋白-3蛋白表达的影响[J]. 中国临床药理学与治疗学, 2012, 17(5): 517-520.

HE Jian-guo, HUANG Chang-shun, JIANG Juan, HU Ye, CAO Gang, LIU Zhen-wei. The impact of propofol pretreatment on hypoxia/reoxygenation in cultured hippocampal neurons metallothionein-3 protein expression[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2012, 17(5): 517-520.

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