外源性血管内皮衍生超极化因子对脑缺血再灌注损伤的影响

中国临床药理学与治疗学 ›› 2012, Vol. 17 ›› Issue (6): 648-653.

外源性血管内皮衍生超极化因子对脑缺血再灌注损伤的影响

焦传安^1,2, 宋标^2,3, 陈志武²

¹巢湖市第一人民医院精神科,巢湖,238000 安徽;
²安徽医科大学药理学教研室,合肥 230032 安徽;
³合肥市第三人民医院药学部,合肥 230022 安徽

收稿日期:2011-12-22 修回日期:2012-05-11 出版日期:2012-06-26 发布日期:2012-06-25
通讯作者: 陈志武,男,博士,教授,博士生导师,研究方向:药理学。Tel: 0551-5161133 E-mail: wzcxiong@mail.hf.ah.cn
作者简介:焦传安,男,学士,副主任医师,研究方向:神经病学和神经药理学。Tel: 0565-3655423 E-mail: jiaochuanan@163.com
基金资助:
国家自然科学基金项目(81173596)和安徽省自然科学基金项目(11040606M196)资助

Effects of exogenous endothelium-dependent hyperpolarizing factor on cerebral ischemia reperfusion injury

JIAO Chuan-an^1,2, SONG Biao^1,3, CHEN Zhi-wu¹

¹Department of Psychiatry, the First People's Hospital of Chaohu, Chaohu, Hefei 238000, Anhui, China;
²Department of Pharmacology, Anhui Medical University, Hefei 230032, Anhui, China;
³Department of Pharmacy, the Third People's Hospital of Hefei, Hefei 230022, Anhui, China

Received:2011-12-22 Revised:2012-05-11 Online:2012-06-26 Published:2012-06-25

摘要/Abstract

摘要： 目的: 硫化氢(hydrogen sulfide, H₂S)为一种假定的血管内皮衍生超极化因子(endothelium- derived hyperpolarizing factor, EDHF),本研究探讨外源性H₂S,即外源性假定的EDHF对脑缺血再灌注损伤的影响。方法: 采用线栓法复制大鼠局灶性脑缺血(MCAO)再灌注损伤模型,测定动物行为功能、脑组织梗死体积、脑组织含水量、血清乳酸脱氢酶(LDH)活性及丙二醛(MDA)含量,用HE染色法观察脑组织学改变。结果: H₂S供体硫氢化钠(NaHS, i.v.) 0.195、0.390、0.780 mg/kg 能明显改善神经功能状态,降低缺血再灌注后脑梗死体积百分比,降低脑含水量,显著地抑制局灶性脑缺血再灌注损伤大鼠血清MDA含量和LDH活性,并不同程度地改善大鼠脑病理组织学的变化。结论: NaHS可明显改善大鼠脑缺血再灌注性损伤,提示外源性EDHF (H₂S)有抗脑缺血再灌损伤作用。

关键词: 内皮依赖性超极化因子, 硫化氢, 硫氢化钠, 脑缺血再灌注损伤

Abstract: AIM: Hydrogen sulfide (H₂S) is one of the supposed endothelium-dependent hyperpolarizing factor (EDHF), the protective effects of exogenous H₂S, namely exogenous supposed EDHF on cerebral ischemia reperfusion (I/R) injury was observed in present study. METHODS: Sodium hydrosulfide (NaHS) was taken as a donor of H₂S. Focal cerebral I/R injury was caused by occluding the right middle cerebral artery (MCAO) in rats. The numerical data, which were from infarct volume, neurological deficit, brain water content, malondialdehyde (MDA) level and lactate dehydrogenase (LDH) activities in serum, were evaluated. HE staining was used to observe brain pathologic changes. RESULTS: 0.195, 0.390, 0.780 mg/kg NaHS markedly ameliorated the neurological deficit, decreased the percentage of infarct volume, reduced the brain water content, and dramatically inhibited the cerebral I/R injury-induced increases of LDH activity and MDA content in serum, improved brain pathologic changes in different degrees. CONCLUSION: NaHS significantly improved the cerebral I/R injury in rats, this suggests exogenous supposed EDHF (H₂S) has a significant protective effect against cerebral I/R injury.

Key words: Endothelium-dependent hyperpolarizing factor, Hydrogen sulfide, Sodium hydrosulfide, Cerebral ischemia reperfusion injury

中图分类号:

R965.2

焦传安, 宋标, 陈志武. 外源性血管内皮衍生超极化因子对脑缺血再灌注损伤的影响[J]. 中国临床药理学与治疗学, 2012, 17(6): 648-653.

JIAO Chuan-an, SONG Biao, CHEN Zhi-wu. Effects of exogenous endothelium-dependent hyperpolarizing factor on cerebral ischemia reperfusion injury[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2012, 17(6): 648-653.

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