匹格列酮抑制小鼠肝切除术后肝脏再生

中国临床药理学与治疗学 ›› 2012, Vol. 17 ›› Issue (8): 851-855.

匹格列酮抑制小鼠肝切除术后肝脏再生

程张军^1,2, 杨平华², 周家华¹, 沈锋²

¹东南大学附属中大医院普外科,南京 210009, 江苏;
²第二军医大学附属东方肝胆外科医院,上海 200438

收稿日期:2012-06-15 修回日期:2012-07-12 出版日期:2012-08-26 发布日期:2012-08-14
通讯作者: 沈锋,男,教授,博士生导师,研究方向:肝脏外科基础与临床。E-mail: shenfengdfgd@yahoo.com.cn
作者简介:程张军,男,医学博士,主治医师,研究方向:肝脏外科基础与临床。Tel: 025-83272195 E-mail: chengzhangjun2003@hotmail.com
基金资助:
国家自然科学基金面上项目(30700801)

Pioglitazone inhibits mouse liver regeneration induced by partial hepatectomy

CHENG Zhang-jun^1,2, YANG Ping-hua², ZHOU Jia-hua¹, SHEN Feng²

¹Department of General Surgery, Zhongda Hospital, Southeast University, Nanjing 210009, Jiangsu, China;
²Eastern Hepatobiliary Sugery Hospital, Second Military Medical University, Shanghai 200438, China

Received:2012-06-15 Revised:2012-07-12 Online:2012-08-26 Published:2012-08-14

摘要/Abstract

摘要： 目的: 探讨匹格列酮在小鼠肝切除术后肝脏再生中的作用。方法: 对C57BL/6J小鼠实施2/3肝切除,建立小鼠肝再生模型。实验组小鼠按体重给予匹格列酮 20 mg·kg^-1·d^-1口服,对照组给予安慰剂口服,在术后不同时间点收集小鼠残余肝脏和血清,计算肝脏体重比;监测术后肝功能和血糖变化;H&E染色观察肝脏形态学变化,免疫组化染色观察肝细胞增殖情况。结果: 匹格列酮 20 mg·kg^-1·d^-1 对小鼠术后肝功能和血糖无明显影响。与对照组相比,匹格列酮组小鼠术后肝脏生长缓慢,肝细胞增殖受到抑制(P＜0.05)。结论: 匹格列酮抑制小鼠肝切除术后肝脏再生。

关键词: 匹格列酮, 肝再生, 肝切除术

Abstract: AIM: To investigate the effects of pioglitazone on mouse liver regeneration induced by partial hepatectomy.METHODS: 2/3 partial hepatectomy was performed in C57BL/6J mice. The experimental group mice were treated with pioglitazone (20 mg·kg^-1·d^-1) by oral gavage. The control group mice were treated with vehicle. Mouse serum and liver tissue harvest were performed at serials time point post operation. The liver/body weight ratio were calculated; liver histology was assessed by H&E staining; serum transaminase and glucose were determined by biochemistry assay. Hepatocellular proliferation was eveluated by IHC staining of Ki67 and PH3.RESULTS: There were no hepatic toxic and side effects caused by pioglitazone under the given dosage. Pioglitazone didn't impair the metabolism of serum glucose. The liver growth and hepatocyte proliferation were delayed and inhibited in pioglitazone treated mice in comparison to that in control mice(P＜0.05).CONCLUSION: Pioglitazone inhibits liver regeneration following partial hepatectomy in mice.

Key words: Pioglitazone, Liver regeneration, Partial hapatectomy

中图分类号:

R965.2

程张军, 杨平华, 周家华, 沈锋. 匹格列酮抑制小鼠肝切除术后肝脏再生[J]. 中国临床药理学与治疗学, 2012, 17(8): 851-855.

CHENG Zhang-jun, YANG Ping-hua, ZHOU Jia-hua, SHEN Feng. Pioglitazone inhibits mouse liver regeneration induced by partial hepatectomy[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2012, 17(8): 851-855.

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