大蒜素对不同年龄组小鼠血清神经递质5-羟色胺和β-内啡肽的影响

中国临床药理学与治疗学 ›› 2012, Vol. 17 ›› Issue (8): 884-887.

大蒜素对不同年龄组小鼠血清神经递质5-羟色胺和β-内啡肽的影响

吕亚囡, 董群

皖南医学院微生物学与免疫学教研室,芜湖 241002,安徽

收稿日期:2012-03-14 修回日期:2012-06-21 出版日期:2012-08-26 发布日期:2012-08-14
通讯作者: 董群,女,教授,硕士生导师,研究方向:天然产物的调节作用。Tel: 13955370176
作者简介:吕亚囡,女,2009级病原生物学专业硕士研究生,研究方向为抗感染免疫。Tel: 15212243202 E-mail: 258187879@qq.com
基金资助:
安徽省教育厅重点项目(kj2010A322)

Effects of garlicin on 5-HT, β-EP of mice at the different age groups

LV Ya-nan, DONG Qun

Department of Microbiology and Immunology, Wannan Medical College, Wuhu 241002, Anhui, China

Received:2012-03-14 Revised:2012-06-21 Online:2012-08-26 Published:2012-08-14

摘要/Abstract

摘要： 目的: 探讨大蒜素对不同年龄组小鼠神经递质5-羟色胺(5-HT)和β-内啡肽(β-EP)的影响。方法: 健康ICR小鼠60只,随机分为青年组和老年组;各组又分设对照组和实验组。老年组鼠是用D-半乳糖制备衰老模型。实验组喂食大蒜素 14 d,对照组灌服同体积生理盐水。采用ELISA法测各组小鼠血清5-HT和β-EP含量。结果: 老年组鼠的β-EP明显低于青年组(P＜0.05),而老年组鼠和青年组鼠5-HT无统计学差异(P＞0.05)。青年实验组鼠较青年对照组鼠的5-HT,β-EP水平明显增加(P＜0.05);老年实验组较老年对照组的β-EP水平明显增加(P＜0.05),而5-HT仅有增加趋势,但差异无统计学意义(P＞0.05)。结论: 大蒜素对β-EP的表达有明显的上调作用,对5-HT的上调作用青年组较老年组明显。

关键词: 大蒜素, 衰老模型, 5-羟色胺, β-内啡肽, 神经内分泌免疫调节网络(NEI)

Abstract: AIM: To explore the effects of garlicin on 5-HT, β-EP of mice at the different age groups.METHODS: The mice were randomly divided into young group and senium group, and each group consists of treatment group and control group. Aging models of mice were with D-galactose. The mice in treatment group were administered orally with garlicin for consecutive 14 days and two other groups with equal amount of normal saline. The content of 5-HT and β-EP were detected by enzyme-linked immunosorbent assay (ELISA).RESULTS: The β-EP content were lower in the senium groups those that in young groups (P＜0.05). The difference of 5-HT was not signifi cant between senium group and young group(P＞0.05). Garlicin improved the levels of 5-HT and β-EP in the young treatment groups(P＜0.05). Compared with senium control group, the level of β-EP was higher in senium treatment group(P＜0.05), but the difference of 5-HT was not signifi cant between the two groups(P＞0.05).CONCLUSION: Garlicin can improve the level of β-EP of mice, and it can improve the level of 5-HT of young mice.

Key words: Garlicin, Aging model, 5-HT, β-EP, Neuro-endocrine-immunoregulatory network(NEI)

中图分类号:

R965.2

吕亚囡, 董群. 大蒜素对不同年龄组小鼠血清神经递质5-羟色胺和β-内啡肽的影响[J]. 中国临床药理学与治疗学, 2012, 17(8): 884-887.

LV Ya-nan, DONG Qun. Effects of garlicin on 5-HT, β-EP of mice at the different age groups[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2012, 17(8): 884-887.

参考文献

[1] 徐小江, 肖文军, 陈庆, 等. 大蒜素抗菌作用及其机制研究进展[J]. 医药导报, 2010, 29(8): 1048- 1051.
[2] 陈晓月, 赵承辉, 刘爽, 等. 大蒜素体外抗菌活性研究[J]. 沈阳农业大学学报, 2008, 39(1): 108- 110.
[3] 邵力钧, 单文卫. 大蒜素的临床应用进展[J]. 中国现代医生, 2009, 47(3): 45-47.
[4] 胡国伟, 董群. 大蒜素对白色念珠菌感染的免疫抑制小鼠IL-4和IgG产生水平的影响[J]. 皖南医学院学报, 2011, 30(3): 185-187.
[5] 熊延靖, 董群. 大蒜素抗白色念珠菌感染的免疫学机制[J]. 中国临床药理学与治疗学, 2010, 15(8): 866-870.
[6] 胡国伟, 董群. 大蒜素对白色念珠菌感染的免疫抑制小鼠巨噬细胞功能的影响[J]. 中国临床药理学与治疗学, 2011, 16(3): 270-273.
[7] 姚建平, 金国琴. 衰老神经内分泌免疫网络的中医药研究进展[J]. 中国中医药信息杂志, 2004, 12(11): 22-24.
[8] 朱亚珍, 朱虹光. D-半乳糖致衰老动物模型的建立及其检测方法[J]. 复旦学报:医学版, 2007, 34(4): 617-619.
[9] 徐叔云, 卞如濂, 陈修. 药理学实验方法[M]. 北京: 人民卫生出版社, 1991:178-179.
[10] Besedovsky HO, del Rey A. Introduction: immune-neuroendocrine network [J]. Front Horm Res, 2002, 29: 1-14.
[11] 张焱, 回世洋. 中医五脏调控系统和神经内分泌免疫调节网络的关系[J]. 中华中医药学刊, 2009, 27(1): 188-189.
[12] De la Fuente M, Hernanz A, Medina S, et al. Characterization of monoaminergic systems in brain regions of prematurely ageing mice[J]. Neurochem Int, 2003, 43(2): 165-172.
[13] Rehman HU, Masson EA. Neuroendocrinology of ageing [J]. Age Ageing, 2001, 30(4): 279-287.
[14] 何海蓉, 张石宁, 李萍, 等. 游泳延缓小鼠衰老的神经内分泌机制[J]. 基础医学与临床, 2001, 21(6): 563-565.
[15] 詹向红, 李伟, 赵君玫, 等. 慢性愤怒应激对大鼠衰老进程及其神经内分泌免疫机制的影响[J]. 中华中医药杂志, 2010, 25(1): 111-113.
[16] 孟宪丽, 李建亚, 张艺, 等. 淫羊藿多糖对老年大鼠神经内分泌免疫调节作用的研究[J]. 中药药理与临床, 1998, 14(3): 19-20.
[17] 李建生, 郭盛典, 赵君玫, 等. 老龄大鼠脑缺血再灌注前列腺素和β-内啡肽的变化[J]. 实用老年医学, 2000, 14(5): 239-241.
[18] 弗瑜, 李淑梅, 李树岩, 等. 高血压病患者血浆β-内啡肽、亮脑啡肽含量及白细胞介素-2水平的临床研究[J]. 白求恩医科大学学报, 1997, 23(5): 503-504.
[19] Baldi E, Conti P, Conti R, et al. Hypernociceptive syndromes and pharmacological inhibition of endogenous opioid degradation [J]. Int J Clin Pharmacol Res, 1986, 6 (3): 193-197.
[20] 何成, 潘小平, 王德岭, 等. β-内啡肽与精氨酸加压素在帕金森病患者脑脊液中的含量变化[J]. 中华神经精神科杂志, 1994, 27(1): 51.