非免疫性过敏反应细胞模型的建立

中国临床药理学与治疗学 ›› 2012, Vol. 17 ›› Issue (9): 1007-1009.

非免疫性过敏反应细胞模型的建立

赵吟, 李钦, 郑晓亮, 张信岳

浙江省医学科学院药物研究所,杭州 310013,浙江

收稿日期:2011-12-06 修回日期:2012-02-28 发布日期:2012-09-25
通讯作者: 张信岳,通信作者,男,研究员,硕士生导师,主要研究方向:新药临床前药理学评价及中药注射剂不良反应机制研究。Tel: 0571-88215600 E-mail: zhangxy2003@yahoo.cn
作者简介:赵吟,女,硕士研究生,研究方向:中药药理学和中药注射剂不良反应机制研究。Tel: 0571-88215600 E-mail:emilyin0922@hotmail.com
基金资助:
浙江省科技厅面上项目资助(2009C33033); 浙江省自然科学基金(C11007D)

Anaphylactoid reaction based on RBL -2H3 cell model in vitro

ZHAO Yin, LI Qin, ZHENG Xiao-liang, ZHANG Xin-yue

Institute of Materia Medica, Zhejiang Academy of Medical Sciences, Hangzhou 310013, Zhejiang,China

Received:2011-12-06 Revised:2012-02-28 Published:2012-09-25

摘要/Abstract

摘要： 目的通过非免疫性刺激物C48/80诱导大鼠嗜碱性粒细胞白血病细胞株RBL-2H3细胞脱颗粒反应正交试验,优化非免疫性过敏反应细胞模型建立条件。方法在不同的孵育时间下,C48/80与不同浓度的RBL-2H3细胞共孵育,通过测定β-氨基己糖苷酶的释放率确定建立非免疫型过敏反应细胞模型的最优实验条件,并且分析不同检测方法间的差异。结果不同浓度、不同孵育时间下的RBL-2H3细胞均可受C48/80诱导发生典型的脱颗粒反应,但不同条件下的脱颗粒程度和β-氨基己糖苷酶释放率都有显著差异。结论当RBL-2H3细胞浓度为2×10⁵ mL、孵育时间 60 min 时,细胞的感受性较好,其脱颗粒程度与药物浓度呈正相关。

关键词: 类过敏反应, RBL-2H3细胞, 脱颗粒, 正交试验

Abstract: AIM: To observe the effects of C48/80 on RBL-2H3 cell degranulation orthogonal test, optimize the experimental conditions and discuss the possible detection methods of anaphylactoid reaction . METHODS: The β-hexosaminidase assay of RBL-2H3 cells incubated with C48/80 were tested by orthogonal test considering two laboratorial factors time and cell concentration.According the results the optimized cell model in vitro were set up and disscus the superior and the inferior aspects between different detection means.RESULTS: Different concentrations and time could make significance difference on RBL-2H3 cell model with degranulation.CONCLUSION: Anaphylactoid reaction based on RBL -2H3 cell model in vitro could be built up on the cell concentration of 2×10⁵ under 60 minutes incubation time , which may be appropriate conditions in order to get stable cell model because the degranualtion was significantly increased and dose-dependent under this condition.

Key words: Anaphylactoid reaction, RBL-2H3 cell, Degranulation, Orthogonal test

中图分类号:

R965.2

赵吟, 李钦, 郑晓亮, 张信岳. 非免疫性过敏反应细胞模型的建立[J]. 中国临床药理学与治疗学, 2012, 17(9): 1007-1009.

ZHAO Yin, LI Qin, ZHENG Xiao-liang, ZHANG Xin-yue. Anaphylactoid reaction based on RBL -2H3 cell model in vitro[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2012, 17(9): 1007-1009.

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