枳壳提取物抗抑郁作用及其机制探讨

中国临床药理学与治疗学 ›› 2013, Vol. 18 ›› Issue (10): 1086-1092.

枳壳提取物抗抑郁作用及其机制探讨

徐颖¹, 冯劼¹, 郭建友²

¹杭州市红十字会医院,杭州 310003,浙江;
²中国科学院心理健康重点实验室,中国科学院心理研究所,北京 100101

收稿日期:2013-05-24 修回日期:2013-08-21 出版日期:2013-10-26 发布日期:2013-09-30
通讯作者: 郭建友,男,博士,研究员,博士生导师,研究方向:精神药理学。Tel: 010-64852787 E-mail: guojy@psych.ac.cn
作者简介:徐颖,女,本科,副主任中药师,研究方向:药理学。Tel: 0571-56108630 E-mail: xuying_t@sina.com
基金资助:
中国科学院知识创新方向性项目(KSCX2-YW-R-254)资助

Mechanism underlying the antidepressant effect of Fructus Aurantii

XU Ying¹, FENG Jie¹, GUO Jian-you²

¹The Hangzhou Red Cross Hospital, Hangzhou 310003,Zhejiang,China;
²Key Laboratory of Mental Health, Institute of Psychology, Chinese Academy of Sciences, Beijing 100101,China

Received:2013-05-24 Revised:2013-08-21 Online:2013-10-26 Published:2013-09-30

摘要/Abstract

摘要： 目的:探讨枳壳乙醇提取物对慢性轻度不可预见性的应激(CUMS)大鼠干预作用及其机制。方法:通过 21 d CUMS方法建立抑郁模型,造模的同时灌胃枳壳乙醇提取物(5、10和 20 g/kg)。采用糖水偏好和强迫游泳评价大鼠抑郁行为,以胃排空和肠推力评价大鼠胃动力变化,采集全血测皮质酮(COR),采用逆转录-聚合酶链反应(RT-PCR)法测定海马糖皮质激素受体(GR)、盐皮质受体(MR) mRNA、皮层和海马的脑源性神经营养因子(BDNF) mRNA表达。结果:与模型组比较,枳壳10、20 g/kg 显著提高抑郁大鼠的糖水偏好(P<0.05,P<0.01),显著延长强迫游泳测试中的不动时间(P<0.05,P<0.01)。枳壳 20 g/kg 显著提高大鼠胃动力(P<0.05)。枳壳10、20 g/kg 显著上调海马GR mRNA表达,降低血浆COR浓度,并上调皮层和海马的BDNF mRNA表达水平(P<0.05,P<0.01)。结论:枳壳乙醇提取物的抗抑郁作用可能与提高大鼠胃动力,上调海马GR mRNA、皮层和海马的BDNF mRNA表达有关。

关键词: 慢性应激, 枳壳, 糖皮质激素受体, 脑源性神经营养因子

Abstract: AIM: To explore the mechanism underlying antidepressant effect of Fructus Aurantii on chronic unpredictable mild stress (CUMS) model of depression in rats.METHODS: The depression animal model was induced by chronic unpredictable mild stress. Sucrose preference test and forced swimming test were measured to assess the depressive behaviors. Moreover, stomach emptying rate and intestines push movement were measured to test the gastric motility. In addition, the serum cortisol was measured by ELISA, mRNA expressions of glucocorticoid receptors (GR), alocorticoid receptors (MR) and brain derived neurotrophic factor (BDNF) in rat brain were tested by RT-PCR.RESULTS: Fructus Aurantii, at dosage of 10, 20 g/kg body weight, significantly increased sucrose preference, prolonged immobility time in forced swimming test, and improved gastric motility. In addition, Fructus Aurantii also increased the expression of GR mRNA expression in hippocampus, and BDNF mRNA expression in hippocampus and cortex of CUMS rats.CONCLUSION: Fructus Aurantii exhibits antidepressant effect via improving gastrointestinal function in rats, attenuating the stimulated function of hypothalamus-pituitary-adrenal axis and activating brain-derived neurotrophic factor in CUMS rat model of depression.

Key words: CUMS, Fructus Aurantii, Glucocorticoid receptor, Brain derived neurotrophic factor

中图分类号:

R965.2

徐颖, 冯劼, 郭建友. 枳壳提取物抗抑郁作用及其机制探讨[J]. 中国临床药理学与治疗学, 2013, 18(10): 1086-1092.

XU Ying, FENG Jie, GUO Jian-you. Mechanism underlying the antidepressant effect of Fructus Aurantii[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2013, 18(10): 1086-1092.

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