不同品系小鼠对mCPP诱导焦虑样行为的差异研究

中国临床药理学与治疗学 ›› 2013, Vol. 18 ›› Issue (11): 1238-1243.

不同品系小鼠对mCPP诱导焦虑样行为的差异研究

闫丽萍, 张立权, 伊慧敏, 周雪君, 王珏, 张琦, 叶夷露

浙江医学高等专科学校药理教研室,杭州 310053,浙江

收稿日期:2013-04-03 修回日期:2013-10-29 出版日期:2013-11-26 发布日期:2013-11-22
通讯作者: 叶夷露,女,硕士,副教授,研究方向:神经精神药理学研究。Tel: 0571-87692678 E-mail: yeyilu1006@126.com
作者简介:闫丽萍,女,大专,研究方向:药学。Tel: 18768159289 E-mail: 1096366525@qq.com
基金资助:
浙江省卫生厅科研基金资助项目(2010KYA011);2012年浙江省大学生科技创新活动计划(新苗人才计划)(2012R434002);浙江医学高等专科学校2011年度科研基金项目(学生项目)(2011XX01)

Differences in anxiety-related behavior induced by mCPP and responses to diazepam in two strains of mice

YAN Li-ping, ZHANG Li-quan, YI Hui-min, ZHOU Xue-jun, WANG Jue, ZHANG Qi, YE Yi-lu

Department of Pharmacology, Zhejiang Medical College, Hangzhou 310053,Zhejiang,China

Received:2013-04-03 Revised:2013-10-29 Online:2013-11-26 Published:2013-11-22

摘要/Abstract

摘要： 目的: 评价BALB/c和ICR小鼠对1-(3-氯苯基)哌嗪单盐酸盐(mCPP)诱导焦虑样行为的差异。方法: 将两品系小鼠各分为四组:生理盐水对照组、mCPP焦虑组、地西泮对照组和地西泮+mCPP组。行为学检测前30 min腹腔注射地西泮、皮下注射mCPP,采用开场实验、明暗穿箱和高架十字迷宫比较两品系小鼠焦虑样行为的差异。结果: 地西泮使焦虑的BALB/c小鼠中央路程比值和中央时间比值增加,对ICR小鼠无作用;两品系小鼠运动总路程有差异。地西泮使焦虑小鼠进入暗箱潜伏期延长、明暗穿箱次数增加、暗箱滞留时间缩短,BALB/c小鼠对地西泮的反应比ICR小鼠更敏感。地西泮可使焦虑小鼠开臂进入次数百分比、开臂停留时间百分比和开臂探臂时间百分比增加,两品系小鼠仅在开臂停留时间和开臂探臂时间上有差异。结论: mCPP诱导焦虑小鼠比正常小鼠对地西泮的敏感性高;BALB/c小鼠焦虑水平较低,探究能力和风险评估能力较高,是抗焦虑药物研究中可供选择的较合适的动物品系。

关键词: 焦虑, 地西泮, 品系差异, 小鼠, 1-(3-氯苯基)哌嗪单盐酸盐

Abstract: AIM: To compare the differences in anxiety-related behavior induced by chlorophenylpiperazinehydrochloride (mCPP) and response to diazepam in BALB/c mice and ICR mice.METHODS: Each were divided into four groups: saline group, mCPP group, diazepam group and (diazepam+mCPP) group. Diazepam was administrated intraperitoneally and mCPP was administrated subcutaeously 30 min before behavioral test. Open-field test, light-dark transition test and elevated plus maze test were used.RESULTS: In open-field test, the central distance ratio and the central time ratio were increased in the (diazepam+mCPP) group of BALB/c mice. But there were no effects on ICR mice.Only was there significant difference on total distance between two mice strains.In light-dark transition test, latency into dark box and number of transitions were increased, and time ratio in dark box was decreased in (diazepam+mCPP) group of two strains mice. The response to diazepam was were more sensitive in BALB/c mice than ICR mice. Number of open-arm entries, time and exploring time ratio in open-arm in the elevated plus maze all increased in (diazepam+mCPP) group of two strains mice. There were differences of time and exploring time in open-arm between them.CONCLUSION: The response to diazepam is more sensitive in anxiety mice than normal mice.BALB/c mice have low level of anxiety, high baseline exploration and risk assessment of a new environment, which is a suitable mice strain to develop anxiolytic drugs.

Key words: Anxiety, Strain difference, Diazepam, Mice, Chlorophenylpiperazinehydrochloride (mCPP)

中图分类号:

R965.2

闫丽萍, 张立权, 伊慧敏, 周雪君, 王珏, 张琦, 叶夷露. 不同品系小鼠对mCPP诱导焦虑样行为的差异研究[J]. 中国临床药理学与治疗学, 2013, 18(11): 1238-1243.

YAN Li-ping, ZHANG Li-quan, YI Hui-min, ZHOU Xue-jun, WANG Jue, ZHANG Qi, YE Yi-lu. Differences in anxiety-related behavior induced by mCPP and responses to diazepam in two strains of mice[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2013, 18(11): 1238-1243.

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